BBa_C0013
1
lacI
Repressor, lacI
2004-01-27T12:00:00Z
2015-08-31T04:07:23Z
www.ncbi.nlm.nih.gov
LacI repressor protein, without SsrA degradation tag. No Barcode.
false
false
_1_
0
24
7
Not in stock
false
false
Yong-Su Jin (Fighting Darwins)
annotation2214005
1
Help:Barcodes
range2214005
1
1087
1111
BBa_B0016
1
BBa_B0016
Terminator (T7 RNAP specific, T_Phi)
2003-12-04T12:00:00Z
2015-08-31T04:07:20Z
Sri Kosuri
Released HQ 2013
T7 gene 1 specific transcriptional terminator
false
true
_1_
0
24
7
In stock
false
false
Sri Kosuri
BBa_B0032
1
BBa_B0032
RBS.3 (medium) -- derivative of BBa_0030
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_41_44_48_46_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). <P>
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation7027
1
BBa_B0032
range7027
1
1
13
annotation1709
1
RBS-3\Weak
range1709
1
1
13
annotation1710
1
RBS
range1710
1
7
10
BBa_I2040
1
BBa_I2040
lacI repressor expression device
2007-05-22T11:00:00Z
2015-08-31T04:07:40Z
n/a
lacI expression is controlled by the engineered, tet repressible promoter, R0040 and a medium strength RBS. A T7 transcription terminator is used downstream which is not effective for E. coli RNAP. This terminator is being used simply because the B0032.C0013.B0016 protein generator will be used in several other constructs relating to VM2.0
false
false
_11_
0
135
84
Not in stock
false
I'm using a T7 terminator here rather than an E. coli terminator. This is something of an accident but mainly because I want to use and understand the protein generator.
false
Bartholomew Canton
component2248677
1
BBa_R4032
component2248681
1
BBa_S03691
annotation2248681
1
BBa_S03691
range2248681
1
82
1248
annotation2248677
1
BBa_R4032
range2248677
1
1
75
BBa_R4032
1
BBa_R4032
PoPS/RiPS Generator. Subparts: R0040. B0032
2006-01-17T12:00:00Z
2015-05-08T01:14:16Z
false
true
_11_1_
0
60
1
Not in stock
false
false
Caitlin Conboy
component1787135
1
BBa_R0040
component1787146
1
BBa_B0032
annotation1787146
1
BBa_B0032
range1787146
1
63
75
annotation1787135
1
BBa_R0040
range1787135
1
1
54
BBa_S03691
1
BBa_S03691
C0013:B0016
2007-05-12T11:00:00Z
2015-05-08T01:14:28Z
false
false
_84_
0
135
84
Not in stock
false
false
Bartholomew Canton
component2243895
1
BBa_B0016
component2243894
1
BBa_C0013
annotation2243895
1
BBa_B0016
range2243895
1
1120
1167
annotation2243894
1
BBa_C0013
range2243894
1
1
1111
BBa_R0040
1
p(tetR)
TetR repressible promoter
2003-01-31T12:00:00Z
2015-05-08T01:14:14Z
Lutz, R., Bujard, H., <em>Nucleic Acids Research</em> (1997) 25, 1203-1210.
Released HQ 2013
Sequence for pTet inverting regulator driven by the TetR protein.</P>
false
true
_1_
0
24
7
In stock
false
<P> <P>BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.<P>
true
June Rhee, Connie Tao, Ty Thomson, Louis Waldman
annotation1986785
1
-35
range1986785
1
20
25
annotation1986787
1
-10
range1986787
1
43
48
annotation1986783
1
TetR 1
range1986783
1
1
19
annotation1986784
1
BBa_R0040
range1986784
1
1
54
annotation1986786
1
TetR 2
range1986786
1
26
44
BBa_R4032_sequence
1
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagtcacacaggaaag
BBa_R0040_sequence
1
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
BBa_C0013_sequence
1
atgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtaataacactgatagtgctagtgtagatcac
BBa_I2040_sequence
1
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagtcacacaggaaagtactagatgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtaataacactgatagtgctagtgtagatcactactagagctagcataaccccttggggcctctaaacgggtcttgaggggttttttg
BBa_S03691_sequence
1
atgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtaataacactgatagtgctagtgtagatcactactagagctagcataaccccttggggcctctaaacgggtcttgaggggttttttg
BBa_B0032_sequence
1
tcacacaggaaag
BBa_B0016_sequence
1
ctagcataaccccttggggcctctaaacgggtcttgaggggttttttg
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z