LOCUS       BBa_I2042               2792 bp    DNA     linear       09-May-2024
DEFINITION  T7 RNAP expression system
ACCESSION   BBa_I2042
VERSION     BBa_I2042.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     RBS             63..75
                     /label=RBS-3\Weak
     RBS             69..72
                     /label=RBS
     misc_feature    63..75
                     /label=BBa_B0032
     RBS             63..75
                     /label=BBa_B0032
     misc_feature    1..75
                     /label=BBa_R4032
     promoter        2579..2606
                     /label=putative E. coli promoter
     CDS             82..2736
                     /label=BBa_I2032
     terminator      2745..2792
                     /label=BBa_B0016
     misc_feature    82..2792
                     /label=BBa_S03693
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agtcacacag gaaagtacta gatgaacacg attaacatcg ctaagaacga cttctctgac
      121 atcgaactgg ctgctatccc gttcaacact ctggctgacc attacggtga gcgtttagct
      181 cgcgaacagt tggcccttga gcatgagtct tacgagatgg gtgaagcacg cttccgcaag
      241 atgtttgagc gtcaacttaa agctggtgag gttgcggata acgctgccgc caagcctctc
      301 atcactaccc tactccctaa gatgattgca cgcatcaacg actggtttga ggaagtgaaa
      361 gctaagcgcg gcaagcgccc gacagccttc cagttcctgc aagaaatcaa gccggaagcc
      421 gtagcgtaca tcaccattaa gaccactctg gcttgcctaa ccagtgctga caatacaacc
      481 gttcaggctg tagcaagcgc aatcggtcgg gccattgagg acgaggctcg cttcggtcgt
      541 atccgtgacc ttgaagctaa gcacttcaag aaaaacgttg aggaacaact caacaagcgc
      601 gtagggcacg tctacaagaa agcatttatg caagttgtcg aggctgacat gctctctaag
      661 ggtctactcg gtggcgaggc gtggtcttcg tggcataagg aagactctat tcatgtagga
      721 gtacgctgca tcgagatgct cattgagtca accggaatgg ttagcttaca ccgccaaaat
      781 gctggcgtag taggtcaaga ctctgagact atcgaactcg cacctgaata cgctgaggct
      841 atcgcaaccc gtgcaggtgc gctggctggc atctctccga tgttccaacc ttgcgtagtt
      901 cctcctaagc cgtggactgg cattactggt ggtggctatt gggctaacgg tcgtcgtcct
      961 ctggcgctgg tgcgtactca cagtaagaaa gcactgatgc gctacgaaga cgtttacatg
     1021 cctgaggtgt acaaagcgat taacattgcg caaaacaccg catggaaaat caacaagaaa
     1081 gtcctagcgg tcgccaacgt aatcaccaag tggaagcatt gtccggtcga ggacatccct
     1141 gcgattgagc gtgaagaact cccgatgaaa ccggaagaca tcgacatgaa tcctgaggct
     1201 ctcaccgcgt ggaaacgtgc tgccgctgct gtgtaccgca aggacaaggc tcgcaagtct
     1261 cgccgtatca gccttgagtt catgcttgag caagccaata agtttgctaa ccataaggcc
     1321 atctggttcc cttacaacat ggactggcgc ggtcgtgttt acgctgtgtc aatgttcaac
     1381 ccgcaaggta acgatatgac caaaggactg cttacgctgg cgaaaggtaa accaatcggt
     1441 aaggaaggtt actactggct gaaaatccac ggtgcaaact gtgcgggtgt cgataaggtt
     1501 ccgttccctg agcgcatcaa gttcattgag gaaaaccacg agaacatcat ggcttgcgct
     1561 aagtctccac tggagaacac ttggtgggct gagcaagatt ctccgttctg cttccttgcg
     1621 ttctgctttg agtacgctgg ggtacagcac cacggcctga gctataactg ctcccttccg
     1681 ctggcgtttg acgggtcttg ctctggcatc cagcacttct ccgcgatgct ccgagatgag
     1741 gtaggtggtc gcgcggttaa cttgcttcct agtgaaaccg ttcaggacat ctacgggatt
     1801 gttgctaaga aagtcaacga gattctacaa gcagacgcaa tcaatgggac cgataacgaa
     1861 gtagttaccg tgaccgatga gaacactggt gaaatctctg agaaagtcaa gctgggcact
     1921 aaggcactgg ctggtcaatg gctggcttac ggtgttactc gcagtgtgac taagcgttca
     1981 gtcatgacgc tggcttacgg gtccaaagag ttcggcttcc gtcaacaagt gctggaagat
     2041 accattcagc cagctattga ttccggcaag ggtctgatgt tcactcagcc gaatcaggct
     2101 gctggataca tggctaagct gatttgggaa tctgtgagcg tgacggtggt agctgcggtt
     2161 gaagcaatga actggcttaa gtctgctgct aagctgctgg ctgctgaggt caaagataag
     2221 aagactggag agattcttcg caagcgttgc gctgtgcatt gggtaactcc tgatggtttc
     2281 cctgtgtggc aggaatacaa gaagcctatt cagacgcgct tgaacctgat gttcctcggt
     2341 cagttccgct tacagcctac cattaacacc aacaaagata gcgagattga tgcacacaaa
     2401 caggagtctg gtatcgctcc taactttgta cacagccaag acggtagcca ccttcgtaag
     2461 actgtagtgt gggcacacga gaagtacgga atcgaatctt ttgcactgat tcacgactcc
     2521 ttcggtacca ttccggctga cgctgcgaac ctgttcaaag cagtgcgcga aactatggtt
     2581 gacacatatg agtcttgtga tgtactggct gatttctacg accagttcgc tgaccagttg
     2641 cacgagtctc aattggacaa aatgccagca cttccggcta aaggtaactt gaacctccgt
     2701 gacatcttag agtcggactt cgcgttcgcg taataatact agagctagca taaccccttg
     2761 gggcctctaa acgggtcttg aggggttttt tg
//