LOCUS       BBa_I2052               1973 bp    DNA     linear       10-May-2024
DEFINITION  mCherry and GFP expression system
ACCESSION   BBa_I2052
VERSION     BBa_I2052.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     stem_loop       64..126
                     /label=hp1
     misc_feature    127..137
                     /label=ss1
     stem_loop       138..166
                     /label=hp2
     misc_feature    167..178
                     /label=ss2
     misc_feature    64..178
                     /label=BBa_B0100
     misc_feature    187..192
                     /label=AvrII/XbaI mixed site
     misc_feature    187..192
                     /label=BBa_B0104
     RBS             201..220
                     /label=BBa_B2022
     CDS             227..937
                     /label=mCherry
     misc_feature    578..578
                     /label=C->T (removing PstI site)
     misc_feature    227..940
                     /label=BBa_J06504
     stem_loop       954..1006
                     /label=stem_loop
     misc_feature    949..1006
                     /label=BBa_B0101
     stem_loop       1026..1069
                     /label=stem_loop
     misc_feature    1015..1094
                     /label=BBa_B0010
     terminator      1015..1094
                     /label=BBa_B0010
     stem_loop       1110..1129
                     /label=T7 TE
     misc_feature    1136..1136
                     /label=stop
     polyA_site      1130..1143
                     /label=polya
     misc_feature    1103..1143
                     /label=BBa_B0012
     terminator      1103..1143
                     /label=BBa_B0012
     promoter        1152..1174
                     /label=BBa_R0183
     RBS             1183..1191
                     /label=BBa_B0073
     CDS             1198..1917
                     /label=GFP protein
     CDS             1198..1917
                     /label=BBa_E0040
     terminator      1926..1973
                     /label=BBa_B0016
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gaggccaggg gtgctcggca taagccgaag atatcggtag agttaatatt gagcagatcc
      121 cccggtgaag gatttaaccg tgttatctcg ttggagatat tcatggcgta ttttggatta
      181 ctagagccta gatactagag aaccctcagg aggtaaacca tactagatgg tgagcaaggg
      241 cgaggaggat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg
      301 ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg
      361 cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat
      421 cctgtcccct cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc
      481 cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga
      541 ggacggcggc gtggtgaccg tgacccagga ctcctccttg caggacggcg agttcatcta
      601 caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac
      661 catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga
      721 gatcaagcag aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac
      781 ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga
      841 catcacctcc cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg
      901 ccactccacc ggcggcatgg acgagctgta caagtaataa tactagagga tcgcctgatc
      961 ccggtgcacc cgggcagctg catagtctgg gtgcaccggg atcaggtact agagccaggc
     1021 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
     1081 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt
     1141 atatactaga gtcatacgac tcactatagg gagatactag agtcacacca ctactagatg
     1201 cgtaaaggag aagaactttt cactggagtt gtcccaattc ttgttgaatt agatggtgat
     1261 gttaatgggc acaaattttc tgtcagtgga gagggtgaag gtgatgcaac atacggaaaa
     1321 cttaccctta aatttatttg cactactgga aaactacctg ttccatggcc aacacttgtc
     1381 actactttcg gttatggtgt tcaatgcttt gcgagatacc cagatcatat gaaacagcat
     1441 gactttttca agagtgccat gcccgaaggt tatgtacagg aaagaactat atttttcaaa
     1501 gatgacggga actacaagac acgtgctgaa gtcaagtttg aaggtgatac ccttgttaat
     1561 agaatcgagt taaaaggtat tgattttaaa gaagatggaa acattcttgg acacaaattg
     1621 gaatacaact ataactcaca caatgtatac atcatggcag acaaacaaaa gaatggaatc
     1681 aaagttaact tcaaaattag acacaacatt gaagatggaa gcgttcaact agcagaccat
     1741 tatcaacaaa atactccaat tggcgatggc cctgtccttt taccagacaa ccattacctg
     1801 tccacacaat ctgccctttc gaaagatccc aacgaaaaga gagaccacat ggtccttctt
     1861 gagtttgtaa cagctgctgg gattacacat ggcatggatg aactatacaa ataataatac
     1921 tagagctagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttg
//