LOCUS       BBa_I2057                862 bp    DNA     linear       27-May-2024
DEFINITION  Promoter Tester with RFP
ACCESSION   BBa_I2057
VERSION     BBa_I2057.1  
FEATURES             Location/Qualifiers
     RBS             1..13
                     /label=RBS-3\Weak
     RBS             7..10
                     /label=RBS
     misc_feature    1..13
                     /label=BBa_B0032
     RBS             1..13
                     /label=BBa_B0032
     CDS             20..694
                     /label=mrfp1
     misc_feature    701..725
                     /label=Help:Barcodes
     CDS             20..700
                     /label=BBa_E1010
     stem_loop       745..788
                     /label=stem_loop
     misc_feature    734..813
                     /label=BBa_B0010
     terminator      734..813
                     /label=BBa_B0010
     stem_loop       829..848
                     /label=T7 TE
     misc_feature    855..855
                     /label=stop
     polyA_site      849..862
                     /label=polya
     misc_feature    822..862
                     /label=BBa_B0012
     terminator      822..862
                     /label=BBa_B0012
     misc_feature    20..862
                     /label=BBa_J04650
ORIGIN
        1 tcacacagga aagtactaga tggcttcctc cgaagacgtt atcaaagagt tcatgcgttt
       61 caaagttcgt atggaaggtt ccgttaacgg tcacgagttc gaaatcgaag gtgaaggtga
      121 aggtcgtccg tacgaaggta cccagaccgc taaactgaaa gttaccaaag gtggtccgct
      181 gccgttcgct tgggacatcc tgtccccgca gttccagtac ggttccaaag cttacgttaa
      241 acacccggct gacatcccgg actacctgaa actgtccttc ccggaaggtt tcaaatggga
      301 acgtgttatg aacttcgaag acggtggtgt tgttaccgtt acccaggact cctccctgca
      361 agacggtgag ttcatctaca aagttaaact gcgtggtacc aacttcccgt ccgacggtcc
      421 ggttatgcag aaaaaaacca tgggttggga agcttccacc gaacgtatgt acccggaaga
      481 cggtgctctg aaaggtgaaa tcaaaatgcg tctgaaactg aaagacggtg gtcactacga
      541 cgctgaagtt aaaaccacct acatggctaa aaaaccggtt cagctgccgg gtgcttacaa
      601 aaccgacatc aaactggaca tcacctccca caacgaagac tacaccatcg ttgaacagta
      661 cgaacgtgct gaaggtcgtc actccaccgg tgcttaataa cgctgatagt gctagtgtag
      721 atcgctacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt
      781 cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg
      841 ggtgggcctt tctgcgttta ta
//