LOCUS       BBa_I23000              1792 bp    DNA     linear       18-May-2024
DEFINITION  RBS Scaffold with mCherry reporter
ACCESSION   BBa_I23000
VERSION     BBa_I23000.1  
FEATURES             Location/Qualifiers
     misc_feature    1..54
                     /label=BBa_R0011
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     stem_loop       56..118
                     /label=hp1
     misc_feature    119..129
                     /label=ss1
     stem_loop       130..158
                     /label=hp2
     misc_feature    159..170
                     /label=ss2
     misc_feature    56..170
                     /label=BBa_B0100
     misc_feature    171..176
                     /label=AvrII site
     misc_feature    171..176
                     /label=BBa_B0048
     misc_feature    1..176
                     /label=BBa_S00159
     CDS             213..518
                     /label=ccdB (rev)
     CDS             520..740
                     /label=ccdA- (rev)
     misc_feature    651..651
                     /label=T to C mutation
     misc_feature    779..784
                     /label=-10 (rev)
     misc_feature    802..807
                     /label=-35 (rev)
     misc_feature    185..859
                     /label=BBa_P1010
     misc_feature    868..874
                     /label=SapI recognition
     misc_feature    868..875
                     /label=BBa_B0102
     CDS             876..1586
                     /label=mCherry
     misc_feature    1227..1227
                     /label=C->T (removing PstI site)
     misc_feature    876..1589
                     /label=BBa_J06504
     misc_feature    868..1589
                     /label=BBa_E0042
     stem_loop       1603..1655
                     /label=stem_loop
     misc_feature    1598..1655
                     /label=BBa_B0101
     stem_loop       1675..1718
                     /label=stem_loop
     misc_feature    1664..1743
                     /label=BBa_B0010
     terminator      1664..1743
                     /label=BBa_B0010
     stem_loop       1759..1778
                     /label=T7 TE
     misc_feature    1785..1785
                     /label=stop
     polyA_site      1779..1792
                     /label=polya
     misc_feature    1752..1792
                     /label=BBa_B0012
     terminator      1752..1792
                     /label=BBa_B0012
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacagccag
       61 gggtgctcgg cataagccga agatatcggt agagttaata ttgagcagat cccccggtga
      121 aggatttaac cgtgttatct cgttggagat attcatggcg tattttggat cctaggtact
      181 agagactggc tgtgtataag ggagcctgac atttatattc cccagaacat caggttaatg
      241 gcgtttttga tgtcattttc gcggtggctg agatcagcca cttcttcccc gataacggag
      301 accggcacac tggccatatc ggtggtcatc atgcgccagc tttcatcccc gatatgcacc
      361 accgggtaaa gttcacggga gactttatct gacagcagac gtgcactggc cagggggatc
      421 accatccgtc gcccgggcgt gtcaataata tcactctgta catccacaaa cagacgataa
      481 cggctctctc ttttataggt gtaaacctta aactgcattt caccagcccc tgttctcgtc
      541 agcaaaagag ccgttcattt caataaaccg ggcgacctca gccatccctt cctgattttc
      601 cgctttccag cgttcggcac gcagacgacg ggcttcattc tgcatggttg tgcttaccag
      661 accggagata ttgacatcat atatgccttg agcaactgat agctgtcgct gtcaactgtc
      721 actgtaatac gctgcttcat agcatacctc tttttgacat acttcgggta tacatatcag
      781 tatatattct tataccgcaa aaatcagcgc gcaaatacgc atactgttat ctggctttta
      841 gtaagccgga tccacgcgtt actagaggct cttctatggt gagcaagggc gaggaggata
      901 acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg
      961 gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg
     1021 ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc
     1081 agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga
     1141 agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg
     1201 tggtgaccgt gacccaggac tcctccttgc aggacggcga gttcatctac aaggtgaagc
     1261 tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg
     1321 aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagcaga
     1381 ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc tacaaggcca
     1441 agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac atcacctccc
     1501 acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg
     1561 gcggcatgga cgagctgtac aagtaataat actagaggat cgcctgatcc cggtgcaccc
     1621 gggcagctgc atagtctggg tgcaccggga tcaggtacta gagccaggca tcaaataaaa
     1681 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct
     1741 ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta ta
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