LOCUS       BBa_I3101                959 bp    DNA     linear       17-May-2024
DEFINITION  TetR trigger device (R1075.B0034.C0040.B0015.R0040)
ACCESSION   BBa_I3101
VERSION     BBa_I3101.1  
FEATURES             Location/Qualifiers
     promoter        35..40
                     /label=-10
     promoter        15..20
                     /label=-35
     promoter        1..49
                     /label=BBa_R1075
     misc_feature    62..65
                     /label=conserved
     RBS             58..69
                     /label=BBa_B0034
     CDS             79..695
                     /label=tetR
     misc_feature    696..729
                     /label=SsrA
     misc_feature    736..760
                     /label=Help:Barcodes
     CDS             76..735
                     /label=BBa_C0040
     stem_loop       780..823
                     /label=stem_loop
     misc_feature    769..848
                     /label=BBa_B0010
     terminator      769..848
                     /label=BBa_B0010
     stem_loop       864..883
                     /label=T7 TE
     misc_feature    890..890
                     /label=stop
     polyA_site      884..897
                     /label=polya
     misc_feature    857..897
                     /label=BBa_B0012
     terminator      857..897
                     /label=BBa_B0012
     misc_feature    906..924
                     /label=TetR 1
     misc_feature    906..959
                     /label=BBa_R0040
     promoter        925..930
                     /label=-35
     misc_feature    931..949
                     /label=TetR 2
     promoter        948..953
                     /label=-10
     promoter        906..959
                     /label=BBa_R0040
ORIGIN
        1 ttaaatttcc tcttttcagg ccggaataac tccctataat gcgccaccat actagagaaa
       61 gaggagaaat actagatgtc cagattagat aaaagtaaag tgattaacag cgcattagag
      121 ctgcttaatg aggtcggaat cgaaggttta acaacccgta aactcgccca gaagctaggt
      181 gtagagcagc ctacattgta ttggcatgta aaaaataagc gggctttgct cgacgcctta
      241 gccattgaga tgttagatag gcaccatact cacttttgcc ctttagaagg ggaaagctgg
      301 caagattttt tacgtaataa cgctaaaagt tttagatgtg ctttactaag tcatcgcgat
      361 ggagcaaaag tacatttagg tacacggcct acagaaaaac agtatgaaac tctcgaaaat
      421 caattagcct ttttatgcca acaaggtttt tcactagaga atgcattata tgcactcagc
      481 gctgtggggc attttacttt aggttgcgta ttggaagatc aagagcatca agtcgctaaa
      541 gaagaaaggg aaacacctac tactgatagt atgccgccat tattacgaca agctatcgaa
      601 ttatttgatc accaaggtgc agagccagcc ttcttattcg gccttgaatt gatcatatgc
      661 ggattagaaa aacaacttaa atgtgaaagt gggtccgctg caaacgacga aaactacgct
      721 ttagtagctt aataacactg atagtgctag tgtagatcac tactagagcc aggcatcaaa
      781 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga
      841 acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttatatac
      901 tagagtccct atcagtgata gagattgaca tccctatcag tgatagagat actgagcac
//