LOCUS BBa_I3600 4067 bp DNA linear 11-May-2024 DEFINITION Test: Chemotaxis response to secreted aspartate, sender cells (I3100.I3200.E0422) ACCESSION BBa_I3600 VERSION BBa_I3600.1 FEATURES Location/Qualifiers promoter 35..40 /label=-10 promoter 15..20 /label=-35 promoter 1..49 /label=BBa_R1075 misc_feature 62..65 /label=conserved RBS 58..69 /label=BBa_B0034 misc_feature 1165..1203 /label=LVA CDS 76..1203 /label=lacI-LVA misc_feature 76..1203 /label=BBa_C0012 misc_feature 1204..1228 /label=Help:Barcodes CDS 76..1203 /label=BBa_C0012 stem_loop 1248..1291 /label=stem_loop misc_feature 1237..1316 /label=BBa_B0010 terminator 1237..1316 /label=BBa_B0010 stem_loop 1332..1351 /label=T7 TE misc_feature 1358..1358 /label=stop polyA_site 1352..1365 /label=polya misc_feature 1325..1365 /label=BBa_B0012 terminator 1325..1365 /label=BBa_B0012 CDS 1374..1461 /label=end of LacI coding region (inactive) misc_feature 1374..1573 /label=BBa_R0010 misc_feature 1462..1499 /label=CAP binding site promoter 1510..1515 /label=-35 promoter 1534..1539 /label=-10 misc_feature 1539..1573 /label=LacI binding site misc_feature 1546..1546 /label=start promoter 1374..1573 /label=BBa_R0010 misc_feature 1586..1589 /label=conserved RBS 1582..1593 /label=BBa_B0034 CDS 1600..3078 /label=AspA misc_feature 1600..1600 /label=G misc_feature 2187..2187 /label=C misc_feature 2223..2223 /label=G misc_feature 2256..2256 /label=C misc_feature 2598..2598 /label=G misc_feature 2724..2724 /label=G misc_feature 3079..3111 /label=LVA misc_feature 3118..3142 /label=Help:Barcodes CDS 1600..3117 /label=BBa_C0083 misc_feature 3155..3158 /label=conserved RBS 3151..3162 /label=BBa_B0034 CDS 3169..3930 /label=CFP (LVA) misc_feature 3887..3924 /label=SsrA misc_feature 3925..3930 /label=2 misc_feature 3237..3237 /label=A misc_feature 3169..3930 /label=BBa_E0022 misc_feature 3169..3930 /label=BBa_E0022 stem_loop 3950..3993 /label=stem_loop misc_feature 3939..4018 /label=BBa_B0010 terminator 3939..4018 /label=BBa_B0010 stem_loop 4034..4053 /label=T7 TE misc_feature 4060..4060 /label=stop polyA_site 4054..4067 /label=polya misc_feature 4027..4067 /label=BBa_B0012 terminator 4027..4067 /label=BBa_B0012 ORIGIN 1 ttaaatttcc tcttttcagg ccggaataac tccctataat gcgccaccat actagagaaa 61 gaggagaaat actagatggt gaatgtgaaa ccagtaacgt tatacgatgt cgcagagtat 121 gccggtgtct cttatcagac cgtttcccgc gtggtgaacc aggccagcca cgtttctgcg 181 aaaacgcggg aaaaagtgga agcggcgatg gcggagctga attacattcc caaccgcgtg 241 gcacaacaac tggcgggcaa acagtcgttg ctgattggcg ttgccacctc cagtctggcc 301 ctgcacgcgc cgtcgcaaat tgtcgcggcg attaaatctc gcgccgatca actgggtgcc 361 agcgtggtgg tgtcgatggt agaacgaagc ggcgtcgaag cctgtaaagc ggcggtgcac 421 aatcttctcg cgcaacgcgt cagtgggctg atcattaact atccgctgga tgaccaggat 481 gccattgctg tggaagctgc ctgcactaat gttccggcgt tatttcttga tgtctctgac 541 cagacaccca tcaacagtat tattttctcc catgaagacg gtacgcgact gggcgtggag 601 catctggtcg cattgggtca ccagcaaatc gcgctgttag cgggcccatt aagttctgtc 661 tcggcgcgtc tgcgtctggc tggctggcat aaatatctca ctcgcaatca aattcagccg 721 atagcggaac gggaaggcga ctggagtgcc atgtccggtt ttcaacaaac catgcaaatg 781 ctgaatgagg gcatcgttcc cactgcgatg ctggttgcca acgatcagat ggcgctgggc 841 gcaatgcgcg ccattaccga gtccgggctg cgcgttggtg cggatatctc ggtagtggga 901 tacgacgata ccgaagacag ctcatgttat atcccgccgt taaccaccat caaacaggat 961 tttcgcctgc tggggcaaac cagcgtggac cgcttgctgc aactctctca gggccaggcg 1021 gtgaagggca atcagctgtt gcccgtctca ctggtgaaaa gaaaaaccac cctggcgccc 1081 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag 1141 gtttcccgac tggaaagcgg gcaggctgca aacgacgaaa actacgcttt agtagcttaa 1201 taactctgat agtgctagtg tagatctcta ctagagccag gcatcaaata aaacgaaagg 1261 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact 1321 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagcaatacg 1381 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc 1441 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc 1501 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata 1561 acaatttcac acatactaga gaaagaggag aaatactaga tgtgtttaaa gcaaatcatt 1621 ggcagcttga aaaagaaggt tcacatgtca aacaacattc gtatcgaaga agatctgttg 1681 ggtaccaggg aagttccagc tgatgcctac tatggtgttc acactctgag agcgattgaa 1741 aacttctata tcagcaacaa caaaatcagt gatattcctg aatttgttcg cggtatggta 1801 atggttaaaa aagccgcagc tatggcaaac aaagagctgc aaaccattcc taaaagtgta 1861 gcgaatgcca tcattgccgc atgtgatgaa gtcctgaaca acggaaaatg catggatcag 1921 ttcccggtag acgtctacca gggcggcgca ggtacttccg taaacatgaa caccaacgaa 1981 gtgctggcca atatcggtct ggaactgatg ggtcaccaaa aaggtgaata tcagtacctg 2041 aacccgaacg accatgttaa caaatgtcag tccactaacg acgcctaccc gaccggtttc 2101 cgtatcgcag tttactcttc cctgattaag ctggtagatg cgattaacca actgcgtgaa 2161 ggctttgaac gtaaagctgt cgaatttcag gacatcctga aaatgggtcg tacccagctg 2221 caagacgcag taccgatgac cctcggtcag gaatttcgcg ctttcagcat cctgctgaaa 2281 gaagaagtga aaaacatcca acgtaccgct gaactgctgc tggaagttaa ccttggtgca 2341 acagcaatcg gtactggtct gaacacgccg aaagagtact ctccgctggc agtgaaaaaa 2401 ctggctgaag ttactggctt cccatgcgta ccggctgaag acctgatcga agcgacctct 2461 gactgcggcg cttatgttat ggttcacggc gcgctgaaac gcctggctgt gaagatgtcc 2521 aaaatctgta acgacctgcg cttgctctct tcaggcccac gtgccggcct gaacgagatc 2581 aacctgccgg aactgcaagc gggctcttcc atcatgccag ctaaagtaaa cccggttgtt 2641 ccggaagtgg ttaaccaggt atgcttcaaa gtcatcggta acgacaccac tgttaccatg 2701 gcagcagaag caggtcagct gcaattgaac gttatggagc cggtcattgg ccaggccatg 2761 ttcgaatccg ttcacattct gaccaacgct tgctacaacc tgctggaaaa atgcattaac 2821 ggcatcactg ctaacaaaga agtgtgcgaa ggttacgttt acaactctat cggtatcgtt 2881 acttacctga acccgttcat cggtcaccac aacggtgaca tcgtgggtaa aatctgtgcc 2941 gaaaccggta agagtgtacg tgaagtcgtt ctggaacgcg gtctgttgac tgaagcggaa 3001 cttgacgata ttttctccgt acagaatctg atgcacccgg cttacaaagc aaaacgctat 3061 actgatgaaa gcgaacaggc tgcaaacgac gaaaactacg ctttagtagc ttaataactc 3121 tgatagtgct agtgtagatc tctactagag aaagaggaga aatactagat ggtgagcaag 3181 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtgaac 3241 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc 3301 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc 3361 ctgacctggg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc 3421 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac 3481 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc 3541 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac 3601 aactacatca gccacaacgt ctatatcacc gccgacaagc agaagaacgg catcaaggcc 3661 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag 3721 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc 3781 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc 3841 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagaggcc tgctgcaaac 3901 gacgaaaact acgctttagt agcttaataa tactagagcc aggcatcaaa taaaacgaaa 3961 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta 4021 ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata //