LOCUS BBa_I4101 4473 bp DNA linear 06-May-2024 DEFINITION Prakash: Segment 1 ACCESSION BBa_I4101 VERSION BBa_I4101.1 FEATURES Location/Qualifiers RBS 1..13 /label=RBS-3\Weak RBS 7..10 /label=RBS misc_feature 1..13 /label=BBa_B0032 RBS 1..13 /label=BBa_B0032 CDS 23..639 /label=tetR misc_feature 640..673 /label=SsrA misc_feature 680..704 /label=Help:Barcodes CDS 20..679 /label=BBa_C0040 stem_loop 724..767 /label=stem_loop misc_feature 713..792 /label=BBa_B0010 terminator 713..792 /label=BBa_B0010 stem_loop 808..827 /label=T7 TE misc_feature 834..834 /label=stop polyA_site 828..841 /label=polya misc_feature 801..841 /label=BBa_B0012 terminator 801..841 /label=BBa_B0012 misc_feature 850..868 /label=TetR 1 misc_feature 850..903 /label=BBa_R0040 promoter 869..874 /label=-35 misc_feature 875..893 /label=TetR 2 promoter 892..897 /label=-10 promoter 850..903 /label=BBa_R0040 RBS 912..924 /label=RBS-3\Weak RBS 918..921 /label=RBS misc_feature 912..924 /label=BBa_B0032 RBS 912..924 /label=BBa_B0032 misc_feature 2020..2058 /label=LVA CDS 931..2058 /label=lacI-LVA misc_feature 931..2058 /label=BBa_C0012 misc_feature 2059..2083 /label=Help:Barcodes CDS 931..2058 /label=BBa_C0012 stem_loop 2103..2146 /label=stem_loop misc_feature 2092..2171 /label=BBa_B0010 terminator 2092..2171 /label=BBa_B0010 stem_loop 2187..2206 /label=T7 TE misc_feature 2213..2213 /label=stop polyA_site 2207..2220 /label=polya misc_feature 2180..2220 /label=BBa_B0012 terminator 2180..2220 /label=BBa_B0012 CDS 2229..2316 /label=end of LacI coding region (inactive) misc_feature 2229..2428 /label=BBa_R0010 misc_feature 2317..2354 /label=CAP binding site promoter 2365..2370 /label=-35 promoter 2389..2394 /label=-10 misc_feature 2394..2428 /label=LacI binding site misc_feature 2401..2401 /label=start promoter 2229..2428 /label=BBa_R0010 RBS 2437..2449 /label=RBS-3\Weak RBS 2443..2446 /label=RBS misc_feature 2437..2449 /label=BBa_B0032 RBS 2437..2449 /label=BBa_B0032 CDS 2459..3166 /label=cI lambda misc_feature 3167..3199 /label=LVA misc_feature 3206..3230 /label=Help:Barcodes CDS 2456..3205 /label=BBa_C0051 stem_loop 3250..3293 /label=stem_loop misc_feature 3239..3318 /label=BBa_B0010 terminator 3239..3318 /label=BBa_B0010 stem_loop 3334..3353 /label=T7 TE misc_feature 3360..3360 /label=stop polyA_site 3354..3367 /label=polya misc_feature 3327..3367 /label=BBa_B0012 terminator 3327..3367 /label=BBa_B0012 misc_feature 3376..3395 /label=LuxR/HSL promoter 3490..3497 /label=-10 promoter 3464..3469 /label=-35 misc_feature 3503..3503 /label=start misc_feature 3505..3524 /label=Putative LuxR/HSL misc_feature 3376..3526 /label=BBa_R0063 promoter 3376..3526 /label=BBa_R0063 RBS 3535..3549 /label=RBS-1\Strong RBS 3542..3546 /label=RBS misc_feature 3535..3549 /label=BBa_B0030 RBS 3535..3549 /label=BBa_B0030 misc_feature 3556..3557 /label=prefix misc_feature 3729..3729 /label=T misc_feature 4047..4047 /label=A CDS 3556..4305 /label=luxR misc_feature 3556..4311 /label=BBa_C0062 misc_feature 4312..4336 /label=Help:Barcodes CDS 3556..4311 /label=BBa_C0062 stem_loop 4356..4399 /label=stem_loop misc_feature 4345..4424 /label=BBa_B0010 terminator 4345..4424 /label=BBa_B0010 stem_loop 4440..4459 /label=T7 TE misc_feature 4466..4466 /label=stop polyA_site 4460..4473 /label=polya misc_feature 4433..4473 /label=BBa_B0012 terminator 4433..4473 /label=BBa_B0012 ORIGIN 1 tcacacagga aagtactaga tgtccagatt agataaaagt aaagtgatta acagcgcatt 61 agagctgctt aatgaggtcg gaatcgaagg tttaacaacc cgtaaactcg cccagaagct 121 aggtgtagag cagcctacat tgtattggca tgtaaaaaat aagcgggctt tgctcgacgc 181 cttagccatt gagatgttag ataggcacca tactcacttt tgccctttag aaggggaaag 241 ctggcaagat tttttacgta ataacgctaa aagttttaga tgtgctttac taagtcatcg 301 cgatggagca aaagtacatt taggtacacg gcctacagaa aaacagtatg aaactctcga 361 aaatcaatta gcctttttat gccaacaagg tttttcacta gagaatgcat tatatgcact 421 cagcgctgtg gggcatttta ctttaggttg cgtattggaa gatcaagagc atcaagtcgc 481 taaagaagaa agggaaacac ctactactga tagtatgccg ccattattac gacaagctat 541 cgaattattt gatcaccaag gtgcagagcc agccttctta ttcggccttg aattgatcat 601 atgcggatta gaaaaacaac ttaaatgtga aagtgggtcc gctgcaaacg acgaaaacta 661 cgctttagta gcttaataac actgatagtg ctagtgtaga tcactactag agccaggcat 721 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg 781 gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat 841 atactagagt ccctatcagt gatagagatt gacatcccta tcagtgatag agatactgag 901 cactactaga gtcacacagg aaagtactag atggtgaatg tgaaaccagt aacgttatac 961 gatgtcgcag agtatgccgg tgtctcttat cagaccgttt cccgcgtggt gaaccaggcc 1021 agccacgttt ctgcgaaaac gcgggaaaaa gtggaagcgg cgatggcgga gctgaattac 1081 attcccaacc gcgtggcaca acaactggcg ggcaaacagt cgttgctgat tggcgttgcc 1141 acctccagtc tggccctgca cgcgccgtcg caaattgtcg cggcgattaa atctcgcgcc 1201 gatcaactgg gtgccagcgt ggtggtgtcg atggtagaac gaagcggcgt cgaagcctgt 1261 aaagcggcgg tgcacaatct tctcgcgcaa cgcgtcagtg ggctgatcat taactatccg 1321 ctggatgacc aggatgccat tgctgtggaa gctgcctgca ctaatgttcc ggcgttattt 1381 cttgatgtct ctgaccagac acccatcaac agtattattt tctcccatga agacggtacg 1441 cgactgggcg tggagcatct ggtcgcattg ggtcaccagc aaatcgcgct gttagcgggc 1501 ccattaagtt ctgtctcggc gcgtctgcgt ctggctggct ggcataaata tctcactcgc 1561 aatcaaattc agccgatagc ggaacgggaa ggcgactgga gtgccatgtc cggttttcaa 1621 caaaccatgc aaatgctgaa tgagggcatc gttcccactg cgatgctggt tgccaacgat 1681 cagatggcgc tgggcgcaat gcgcgccatt accgagtccg ggctgcgcgt tggtgcggat 1741 atctcggtag tgggatacga cgataccgaa gacagctcat gttatatccc gccgttaacc 1801 accatcaaac aggattttcg cctgctgggg caaaccagcg tggaccgctt gctgcaactc 1861 tctcagggcc aggcggtgaa gggcaatcag ctgttgcccg tctcactggt gaaaagaaaa 1921 accaccctgg cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg 1981 cagctggcac gacaggtttc ccgactggaa agcgggcagg ctgcaaacga cgaaaactac 2041 gctttagtag cttaataact ctgatagtgc tagtgtagat ctctactaga gccaggcatc 2101 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg 2161 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata 2221 tactagagca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg 2281 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta 2341 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg 2401 aattgtgagc ggataacaat ttcacacata ctagagtcac acaggaaagt actagatgag 2461 cacaaaaaag aaaccattaa cacaagagca gcttgaggac gcacgtcgcc ttaaagcaat 2521 ttatgaaaaa aagaaaaatg aacttggctt atcccaggaa tctgtcgcag acaagatggg 2581 gatggggcag tcaggcgttg gtgctttatt taatggcatc aatgcattaa atgcttataa 2641 cgccgcattg cttgcaaaaa ttctcaaagt tagcgttgaa gaatttagcc cttcaatcgc 2701 cagagaaatc tacgagatgt atgaagcggt tagtatgcag ccgtcactta gaagtgagta 2761 tgagtaccct gttttttctc atgttcaggc agggatgttc tcacctgagc ttagaacctt 2821 taccaaaggt gatgcggaga gatgggtaag cacaaccaaa aaagccagtg attctgcatt 2881 ctggcttgag gttgaaggta attccatgac cgcaccaaca ggctccaagc caagctttcc 2941 tgacggaatg ttaattctcg ttgaccctga gcaggctgtt gagccaggtg atttctgcat 3001 agccagactt gggggtgatg agtttacctt caagaaactg atcagggata gcggtcaggt 3061 gtttttacaa ccactaaacc cacagtaccc aatgatccca tgcaatgaga gttgttccgt 3121 tgtggggaaa gttatcgcta gtcagtggcc tgaagagacg tttggcgctg caaacgacga 3181 aaactacgct ttagtagctt aataacgctg atagtgctag tgtagatcgc tactagagcc 3241 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt 3301 ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc 3361 gtttatatac tagagacctg tacgatccta caggtgctta tgttaagtaa ttgtattccc 3421 agcgatacaa tagtgtgaca aaaatccaat ttattagaat caaatgtcaa tccattaccg 3481 ttttaatgat atataacacg caaaacttgc gacaaacaat aggtaatact agagattaaa 3541 gaggagaaat actagatgaa aaacataaat gccgacgaca catacagaat aattaataaa 3601 attaaagctt gtagaagcaa taatgatatt aatcaatgct tatctgatat gactaaaatg 3661 gtacattgtg aatattattt actcgcgatc atttatcctc attctatggt taaatctgat 3721 atttcaatcc tagataatta ccctaaaaaa tggaggcaat attatgatga cgctaattta 3781 ataaaatatg atcctatagt agattattct aactccaatc attcaccaat taattggaat 3841 atatttgaaa acaatgctgt aaataaaaaa tctccaaatg taattaaaga agcgaaaaca 3901 tcaggtctta tcactgggtt tagtttccct attcatacgg ctaacaatgg cttcggaatg 3961 cttagttttg cacattcaga aaaagacaac tatatagata gtttattttt acatgcgtgt 4021 atgaacatac cattaattgt tccttctcta gttgataatt atcgaaaaat aaatatagca 4081 aataataaat caaacaacga tttaaccaaa agagaaaaag aatgtttagc gtgggcatgc 4141 gaaggaaaaa gctcttggga tatttcaaaa atattaggtt gcagtgagcg tactgtcact 4201 ttccatttaa ccaatgcgca aatgaaactc aatacaacaa accgctgcca aagtatttct 4261 aaagcaattt taacaggagc aattgattgc ccatacttta aaaattaata acactgatag 4321 tgctagtgta gatcactact agagccaggc atcaaataaa acgaaaggct cagtcgaaag 4381 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg 4441 gctcaccttc gggtgggcct ttctgcgttt ata //