LOCUS BBa_I4103 2952 bp DNA linear 04-May-2024 DEFINITION Prakash: Segment 3 ACCESSION BBa_I4103 VERSION BBa_I4103.1 FEATURES Location/Qualifiers RBS 1..15 /label=RBS-1\Strong RBS 8..12 /label=RBS misc_feature 1..15 /label=BBa_B0030 RBS 1..15 /label=BBa_B0030 misc_feature 22..24 /label=start misc_feature 805..810 /label=2 misc_feature 772..804 /label=LVA CDS 22..771 /label=aiiA misc_feature 22..810 /label=BBa_C0060 misc_feature 811..835 /label=Help:Barcodes CDS 22..810 /label=BBa_C0060 stem_loop 855..898 /label=stem_loop misc_feature 844..923 /label=BBa_B0010 terminator 844..923 /label=BBa_B0010 stem_loop 939..958 /label=T7 TE misc_feature 965..965 /label=stop polyA_site 959..972 /label=polya misc_feature 932..972 /label=BBa_B0012 terminator 932..972 /label=BBa_B0012 misc_feature 981..999 /label=TetR 1 misc_feature 981..1034 /label=BBa_R0040 promoter 1000..1005 /label=-35 misc_feature 1006..1024 /label=TetR 2 promoter 1023..1028 /label=-10 promoter 981..1034 /label=BBa_R0040 RBS 1043..1057 /label=RBS-1\Strong RBS 1050..1054 /label=RBS misc_feature 1043..1057 /label=BBa_B0030 RBS 1043..1057 /label=BBa_B0030 CDS 1064..1825 /label=CFP (LVA) misc_feature 1782..1819 /label=SsrA misc_feature 1820..1825 /label=2 misc_feature 1132..1132 /label=A misc_feature 1064..1825 /label=BBa_E0022 misc_feature 1064..1825 /label=BBa_E0022 stem_loop 1845..1888 /label=stem_loop misc_feature 1834..1913 /label=BBa_B0010 terminator 1834..1913 /label=BBa_B0010 stem_loop 1929..1948 /label=T7 TE misc_feature 1955..1955 /label=stop polyA_site 1949..1962 /label=polya misc_feature 1922..1962 /label=BBa_B0012 terminator 1922..1962 /label=BBa_B0012 misc_feature 1971..1989 /label=TetR 1 misc_feature 1971..2024 /label=BBa_R0040 promoter 1990..1995 /label=-35 misc_feature 1996..2014 /label=TetR 2 promoter 2013..2018 /label=-10 promoter 1971..2024 /label=BBa_R0040 RBS 2033..2047 /label=RBS-1\Strong RBS 2040..2044 /label=RBS misc_feature 2033..2047 /label=BBa_B0030 RBS 2033..2047 /label=BBa_B0030 CDS 2054..2815 /label=YFP (LVA) misc_feature 2810..2815 /label=2 misc_feature 2771..2809 /label=SsrA misc_feature 2295..2295 /label=A (Q->L) misc_feature 2054..2815 /label=BBa_E0032 misc_feature 2054..2815 /label=BBa_E0032 stem_loop 2835..2878 /label=stem_loop misc_feature 2824..2903 /label=BBa_B0010 terminator 2824..2903 /label=BBa_B0010 stem_loop 2919..2938 /label=T7 TE misc_feature 2945..2945 /label=stop polyA_site 2939..2952 /label=polya misc_feature 2912..2952 /label=BBa_B0012 terminator 2912..2952 /label=BBa_B0012 ORIGIN 1 attaaagagg agaaatacta gatgacagta aagaagcttt atttcgtccc agcaggtcgt 61 tgtatgttgg atcattcgtc tgttaatagt acattaacac caggagaatt attagactta 121 ccggtttggt gttatctttt ggagactgaa gaaggaccta ttttagtaga tacaggtatg 181 ccagaaagtg cagttaataa tgaaggtctt tttaacggta catttgtcga agggcaggtt 241 ttaccgaaaa tgactgaaga agatagaatc gtgaatattt taaaacgggt tggttatgag 301 ccggaagacc ttctttatat tattagttct cacttgcatt ttgatcatgc aggaggaaat 361 ggcgctttta taaatacacc aatcattgta cagcgtgctg aatatgaggc ggcgcagcat 421 agcgaagaat atttgaaaga atgtatattg ccgaatttaa actacaaaat cattgaaggt 481 gattatgaag tcgtaccagg agttcaatta ttgcatacac caggccatac tccagggcat 541 caatcgctat taattgagac agaaaaatcc ggtcctgtat tattaacgat tgatgcatcg 601 tatacgaaag agaattttga aaatgaagtg ccatttgcgg gatttgattc agaattagct 661 ttatcttcaa ttaaacgttt aaaagaagtg gtgatgaaag agaagccgat tgttttcttt 721 ggacatgata tagagcagga aaggggatgt aaagtgttcc ctgaatatat agctgcaaac 781 gacgaaaact acgctttagt agcttaataa cgctgatagt gctagtgtag atcgctacta 841 gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct 901 gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt 961 tctgcgttta tatactagag tccctatcag tgatagagat tgacatccct atcagtgata 1021 gagatactga gcactactag agattaaaga ggagaaatac tagatggtga gcaagggcga 1081 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg tgaacggcca 1141 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa 1201 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac 1261 ctggggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa 1321 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa 1381 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct 1441 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta 1501 catcagccac aacgtctata tcaccgccga caagcagaag aacggcatca aggccaactt 1561 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa 1621 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc 1681 cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac 1741 cgccgccggg atcactctcg gcatggacga gctgtacaag aggcctgctg caaacgacga 1801 aaactacgct ttagtagctt aataatacta gagccaggca tcaaataaaa cgaaaggctc 1861 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga 1921 gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta tatactagag tccctatcag 1981 tgatagagat tgacatccct atcagtgata gagatactga gcactactag agattaaaga 2041 ggagaaatac tagatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct 2101 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg 2161 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt 2221 gccctggccc accctcgtga ccaccttcgg ctacggcctg caatgcttcg cccgctaccc 2281 cgaccacatg aagctgcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga 2341 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga 2401 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa 2461 catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga 2521 caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag 2581 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct 2641 gcccgacaac cactacctga gctaccagtc cgccctgagc aaagacccca acgagaagcg 2701 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga 2761 gctgtacaag aggcctgctg caaacgacga aaactacgct ttagtagctt aataatacta 2821 gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct 2881 gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt 2941 tctgcgttta ta //