LOCUS BBa_I50001 4640 bp DNA linear 03-May-2024 DEFINITION F plasmid backbone with BioBrick sites removed, reverse ACCESSION BBa_I50001 VERSION BBa_I50001.1 FEATURES Location/Qualifiers misc_feature 1..703 /label=sopC (IncD) repeat region misc_feature 930..930 /label=A to T mutation (eliminates SpeI site) CDS 777..1748 /label=parB misc_feature 2088..2088 /label=C to T mutation (eliminates PstI site) CDS 1748..2923 /label=parA misc_feature 1..2921 /label=sequence derived from F plasmid in GenBank Accession Number AP001918 misc_feature 3251..4640 /label=sequence derived from F plasmid in GenBank Accession Number AP001918 misc_feature 3628..3628 /label=C to T mutation (eliminates PstI site) CDS 3502..4257 /label=repE misc_feature 4354..4373 /label=iteron (repeat region) misc_feature 4376..4395 /label=iteron (repeat region) misc_feature 4409..4428 /label=iteron (repeat region) misc_feature 4430..4449 /label=iteron (repeat region) misc_feature 4458..4458 /label=C to G mutation (eliminates XbaI site, noncoding region) misc_feature 4351..4640 /label=ori2 (oriS) single copy replication origin polyA_site 4494..4540 /label=A/T region misc_feature 4544..4552 /label=DnaA box misc_feature 4553..4560 /label=DnaA box ORIGIN 1 acttgcatcg gatgcagccc ggtgaacgtg ccggcacggc ctgggtaacc aggtattttg 61 tccacataac cgtgcgcaaa atgttgtgga taagcaggac acagcagcaa tccacagcag 121 gcatacaacc gcacaccgag gttactccgt tctacaggtt acgacgacat gtcaatactt 181 gcccttgaca ggcattgatg gaatcgtagt ctcacgctga tagtctgatc gacaatacaa 241 gtgggaccgt ggtcccagac cgataatcag accgacaaca cgagtgggat cgtggtccca 301 gactaataat cagaccgacg atacgagtgg gaccgtggtc ccagactaat aatcagaccg 361 acgatacgag tgggaccgtg gttccagact aataatcaga ccgacgatac gagtgggacc 421 gtggtcccag actaataatc agaccgacga tacgagtggg accatggtcc cagactaata 481 atcagaccga cgatacgagt gggaccgtgg tcccagtctg attatcagac cgacgatacg 541 agtgggaccg tggtcccaga ctaataatca gaccgacgat acgagtggga ccgtggtccc 601 agactaataa tcagaccgac gatacgagtg ggaccgtggt cccagtctga ttatcagacc 661 gacgatacaa gtggaacagt gggcccagag agaatattca ggccagttat gctttctggc 721 ctgtaacaaa ggacattaag taaagacaga taaacgtaga ctaaaacgtg gtcgcatcag 781 ggtgctggct tttcaagttc cttaagaatg gcctcaattt tctctataca ctcagttgga 841 acacgagacc tgtccaggtt aagcaccatt ttatcgccct tatacaatac tgtcgctcca 901 ggagcaaact gatgtcgtga gcttaaactt gttcttgatg cagatgacgt tttaagcaca 961 gaagttaaaa gagtgataac ttcttcagct tcaaatatca ccccagcttt tttctgctca 1021 tgaaggttag atgcctgctg cttaagtaat tcctctttat ctgtaaaggc tttttgaagt 1081 gcatcacctg accgggcaga tagttcaccg gggtgagaaa aaagagcaac aactgattta 1141 ggcaatttgg cggtgttgat acagcgggta ataatcttac gtgaaatatt ttccgcatca 1201 gccagcgcag aaatatttcc agcaaattca ttctgcaatc ggcttgcata acgctgacca 1261 cgttcataag cacttgttgg gcgataatcg ttacccaatc tggataatgc agccatctgc 1321 tcatcatcca gctcgccaac cagaacacga taatcacttt cggtaagtgc agcagcttta 1381 cgacggcgac tcccatcggc aatttctatg acaccagata ctcttcgacc gaacgccggt 1441 gtctgttgac cagtcagtag aaaagaaggg atgagatcat ccagtgcgtc ctcagtaagc 1501 agctcctggt cacgttcatt acctgaccat acccgagagg tcttctcaac actatcaccc 1561 cggagcactt caagagtaaa cttcacatcc cgaccacata caggcaaagt aatggcatta 1621 ccgcgagcca ttactcctac gcgcgcaatt aacgaatcca ccatcggggc agctggtgtc 1681 gataacgaag tatcttcaac cggttgagta ttgagcgtat gttttggaat aacaggcgca 1741 cgcttcatta tctaatctcc cagcgtggtt taatcagacg atcgaaaatt tcattgcaga 1801 caggttccca aatagaaaga gcatttctcc aggcaccagt tgaagagcgt tgatcaatgg 1861 cctgttcaaa aacagttctc atccggatct gacctttacc aacttcatcc gtttcacgta 1921 caacattttt tagaaccatg cttccccagg catcccgaat ttgctcctcc atccacgggg 1981 actgagagcc attactattg ctgtatttgg taagcaaaat acgtacatca ggctcgaacc 2041 ctttaagatc aacgttcttg agcagatcac gaagcatatc gaaaaattgc agtgcggagg 2101 tgtagtcaaa caactcagca ggcgtgggaa caatcagcac atcagcagca catacgacat 2161 taatcgtgcc gatacccagg ttaggcgcgc tgtcaataac tatgacatca tagtcatgag 2221 caacagtttc aatggccagt cggagcatca ggtgtggatc ggtgggcagt ttaccttcat 2281 caaatttgcc cattaactca gtttcaatac ggtgcagagc cagacaggaa ggaataatgt 2341 caagccccgg ccagcaagtg ggctttattg cataagtgac atcgtccttt tccccaagat 2401 agaaaggcag gagagtgtct tctgcatgaa tatgaagatc tggtacccat ccgtgataca 2461 ttgaggctgt tccctggggg tcgttacctt ccacgagcaa aacacgtagc cccttcagag 2521 ccagatcctg agcaagatga acagaaactg aggttttgta aacgccacct ttatgggcag 2581 caaccccgat caccggtgga aatacgtctt cagcacgtcg caatcgcgta ccaaacacat 2641 cacgcatatg attaatttgt tcaattgtat aaccaacacg ttgctcaacc cgtcctcgaa 2701 tttccatatc cgggtgcggt agtcgccctg ctttctcggc atctctgata gcctgagaag 2761 aaaccccaac taaatccgct gcttcaccta ttctccagcg ccgggttatt ttcctcgctt 2821 ccgggctgtc atcattaaac tgtgcaatgg cgatagcctt cgtcatttca tgaccagcgt 2881 ttatgcactg gttaagtgtt tccatgagtt tcattctgaa catcctttaa tcattgcttt 2941 gcgttttttt attaaatctt gcaatttact gcaaagcaac aacaaaatcg caaagtcatc 3001 aaaaaaccgc aaagttgttt aaaataagag caacactaca aaaggagata agaagagcac 3061 atacctcagt cacttattat cactagcgct cgccgcagcc gtgtaaccga gcatagcgag 3121 cgaactggcg aggaagcaaa gaagaactgt tctgtcagat agctcttacg ctcagcgcaa 3181 gaagaaatat ccaccgtggg aaaaactcca ggtagaggta cacacgcgga tagccaattc 3241 agagtaataa actgtgataa tcaaccctca tcaatgatga cgaactaacc cccgatatca 3301 agtcacatga cgaagggaaa gagaaggaaa tcaactgtga caaactgccc tcaaatttgg 3361 cttccttaaa aattacagtt caaaaagtat gagaaaatcc atgcaggctg aaggaaacag 3421 caaaactgtg acaaattacc ctcagtaggt cagaacaaat gtgacgaacc accctcaaat 3481 ctgtgacaga taaccctcag actatcctgt cgtcatggaa gtgatatcgc ggaaggaaaa 3541 tacgatatga gtcgtctggc ggcctttctt tttctcaatg tatgagaggc gcattggagt 3601 tctgctgttg atctcattaa cacagacttg caggaagcgg cggcggaagt caggcatacg 3661 ctggtaactt tgaggcagct ggtaacgctc tatgatccag tcgattttca gagagacgat 3721 gcctgagcca tccggcttac gatactgaca cagggattcg tataaacgca tggcatacgg 3781 attggtgatt tcttttgttt cactaagccg aaactgcgta aaccggttct gtaacccgat 3841 aaagaaggga atgagatatg ggttgatatg tacactgtaa agccctctgg atggactgtg 3901 cgcacgtttg ataaaccaag gaaaagattc atagcctttt tcatcgccgg catcctcttc 3961 agggcgataa aaaaccactt ccttccccgc gaaactcttc aatgcctgcc gtatatcctt 4021 actggcttcc gcagaggtca atccgaatat ttcagcatat ttagcaacat ggatctcgca 4081 gataccgtca tgttcctgta gggtgccatc agattttctg atctggtcaa cgaacagata 4141 cagcatacgt ttttgatccc gggagagact atatgccgcc tcagtgaggt cgtttgactg 4201 gacgattcgc gggctatttt tacgtttctt gtgattgata accgctgttt ccgccatgac 4261 agatccatgt gaagtgtgac aagtttttag attgtcacac taaataaaaa agagtcaata 4321 agcagggata actttgtgaa aaaacagctt cttctgaggg caatttgtca cagggttaag 4381 ggcaatttgt cacagacagg actgtcattt gagggtgatt tgtcacactg aaagggcaat 4441 ttgtcacaac accttctgta gaaccagcat ggataaaggc ctacaaggcg ctctaaaaaa 4501 gaagatctaa aaactataaa aaaaataatt ataaaaatat ccccgtggat aagtggataa 4561 ccccaaggga agttttttca ggcatcgtgt gtaagcagaa tatataagtg ctgttccctg 4621 gtgcttcctc gctcactcga //