LOCUS       BBa_I50020               674 bp    DNA     linear       02-May-2024
DEFINITION  Minimal pUC19-derived high copy replication origin
ACCESSION   BBa_I50020
VERSION     BBa_I50020.1  
FEATURES             Location/Qualifiers
     misc_feature    16..630
                     /label=rep (pMB1)
     misc_feature    615..615
                     /label=ORI
     misc_feature    27..615
                     /label=origin of replication
     misc_feature    63..615
                     /label=RNAII transcript
     promoter        204..209
                     /label=RNAI transcript promoter -35
     promoter        182..187
                     /label=RNAI transcript promoter -10
     misc_feature    66..173
                     /label=RNAI transcript
     promoter        48..53
                     /label=RNAII transcript promoter -10 sequence
     promoter        27..32
                     /label=RNAII transcript promoter -35 sequence
     misc_feature    261..261
                     /label=G to A, nonfunctional mutation
     misc_feature    265..265
                     /label=G to A, nonfunctional mutation
     misc_feature    274..274
                     /label=G to A, nonfunctional mutation
     misc_feature    303..303
                     /label=C to T, nonfunctional mutation
     misc_feature    467..467
                     /label=C to T, nonfunctional mutation
ORIGIN
        1 cccgtagaaa agatcaaaag atcttcttga gatccttttt ttctgcgcgt aatctgctac
       61 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
      121 actctttttc cgaaggtaac tggctttagc agagcgcaga taccaaatac tgtccttcta
      181 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
      241 ctgctaatcc tgttaccagt agctactgcc agtagcgata agtcgtgtct taccgggttg
      301 gattcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
      361 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
      421 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatctggt aagcggcagg
      481 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
      541 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
      601 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
      661 ccttttgctc acat
//