pUC19 ori

BBa_I50022 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_I50022
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Reshma Shetty
Date created: 2008-01-05 12:00:00
Date modified: 2015-08-31 04:07:42

Minimal pUC19-derived high copy replication origin



Types
DnaRegion

Roles
sequence_feature

DNA

Sequences BBa_I50022_sequence (Version 1)

Description

High copy origin of replication derived from pSB1A3. Designed to omit antibiotic resistance markers and primer binding sites. Lacks mutations that render BBa_I50020 nonfunctional as a replication origin.

Notes

We had to leave several restriction sites in because point mutations that eliminated these sites were found to render the origin nonfunctional.

Source

pSB1A3

Sequence Annotation Location Component / Role(s)
rep (pMB1)
RNAII transcript promoter -35 sequence
origin of replication
RNAII transcript promoter -10 sequence
RNAII transcript
RNAI transcript
RNAI transcript promoter -10
RNAI transcript promoter -35
ORI
16,630
27,32
27,615
48,53
63,615
66,173
182,187
204,209
615,615
sequence_feature feature/misc
feature/promoter promoter
feature/misc sequence_feature
feature/promoter promoter
feature/misc sequence_feature
sequence_feature feature/misc
promoter feature/promoter
promoter feature/promoter
sequence_feature feature/misc
igem#experience
Works
 
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_I50022/1