Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Reshma Shetty
Date created: 2006-02-03 12:00:00
Date modified: 2015-08-31 04:07:42
nonfunctional pSC101 origin of replication
| Types | DnaRegion |
| Roles | sequence_feature DNA |
| Sequences | BBa_I50040_sequence (Version 1) |
Description
pSC101 origin of replication which is supposed to have low copy number. This was the origin used in Michael Elowitz's repressilator and in Bujard's pZS4Int-1 vector with mutations introduced to eliminate a SpeI site. It is also the origin of replication in pSB4* series plasmids.| Sequence Annotation | Location | Component / Role(s) |
| inverted repeat par region inverted repeat 11bp sequence repeated 4 times in oriC AT rich region direct repeat direct repeat direct repeat inverted repeat promoter region potential hairpin -10 signal functional promoter RBS inverted repeat -10 signal functional promoter repA potential hairpin potential hairpin A->T mutation to remove MfeI site T->A mutation to remove MfeI site | 180,189 1,368 191,201 482,492 503,581 578,601 603,620 631,654 702,723 710,729 683,761 723,727 734,746 726,749 948,953 750,1700 1608,1682 2063,2088 708,708 742,742 | sequence_feature feature/misc feature/misc sequence_feature feature/misc sequence_feature sequence_feature feature/misc sequence_feature feature/misc feature/misc sequence_feature sequence_feature feature/misc sequence_feature feature/misc sequence_feature feature/misc feature/promoter promoter feature/stem_loop stem_loop promoter feature/promoter ribosome_entry_site feature/rbs feature/misc sequence_feature feature/promoter promoter feature/cds CDS stem_loop feature/stem_loop feature/stem_loop stem_loop feature/mutation sequence_alteration feature/mutation sequence_alteration |