LOCUS       BBa_I50042              2037 bp    DNA     linear       30-Apr-2024
DEFINITION  pSC101 origin of replication
ACCESSION   BBa_I50042
VERSION     BBa_I50042.1  
FEATURES             Location/Qualifiers
     stem_loop       46..75
                     /label=Terminator
     misc_feature    137..504
                     /label=par region
     misc_feature    139..152
                     /label=inverted repeat
     misc_feature    158..162
                     /label=Tn3 integration site
     misc_feature    165..181
                     /label=inverted repeat
     misc_feature    192..215
                     /label=inverted repeat
     misc_feature    221..238
                     /label=inverted repeat
     misc_feature    635..717
                     /label=AT rich region
     misc_feature    716..735
                     /label=direct repeat
     misc_feature    737..756
                     /label=direct repeat
     misc_feature    769..788
                     /label=direct repeat
     misc_feature    814..819
                     /label=mutated SpeI site
     promoter        828..833
                     /label=-35
     misc_feature    838..859
                     /label=inverted repeat
     promoter        846..865
                     /label=promoter region
     promoter        850..855
                     /label=-10
     misc_feature    862..884
                     /label=inverted repeat
     RBS             870..882
                     /label=RBS
     RBS             879..884
                     /label=RBS
     CDS             886..1836
                     /label=repA
     misc_feature    1655..1655
                     /label=G to A difference with canonical pSC101 vector 
                     sequence
     misc_feature    1750..1750
                     /label=C to T difference with canonical pSC101 vector 
                     sequence
     misc_feature    1847..1861
                     /label=inverted repeat
     misc_feature    1912..1912
                     /label=G to C difference with canonical pSC101 vector 
                     sequence
     polyA_site      1947..1962
                     /label=putative polyA
     misc_feature    1963..1979
                     /label=inverted repeat
     misc_feature    2002..2002
                     /label=T to C difference with canonical pSC101 vector 
                     sequence
ORIGIN
        1 ctgtcagacc aagtttacga gctcgcttgg actcctgttg atagatccag taatgacctc
       61 agaactccat ctggatttgt tcagaacgct cggttgccgc cgggcgtttt ttattggtga
      121 gaatccaagc actagggaca gtaagacggg taagcctgtt gatgataccg ctgccttact
      181 gggtgcatta gccagtctga atgacctgtc acgggataat ccgaagtggt cagactggaa
      241 aatcagaggg caggaactgc tgaacagcaa aaagtcagat agcaccacat agcagacccg
      301 ccataaaacg ccctgagaag cccgtgacgg gcttttcttg tattatgggt agtttccttg
      361 catgaatcca taaaaggcgc ctgtagtgcc atttaccccc attcactgcc agagccgtga
      421 gcgcagcgaa ctgaatgtca cgaaaaagac agcgactcag gtgcctgatg gtcggagaca
      481 aaaggaatat tcagcgattt gcccgagctt gcgagggtgc tacttaagcc tttagggttt
      541 taaggtctgt tttgtagagg agcaaacagc gtttgcgaca tccttttgta atactgcgga
      601 actgactaaa gtagtgagtt atacacaggg ctgggatcta ttctttttat ctttttttat
      661 tctttcttta ttctataaat tataaccact tgaatataaa caaaaaaaac acacaaaggt
      721 ctagcggaat ttacagaggg tctagcagaa tttacaagtt ttccagcaaa ggtctagcag
      781 aatttacaga tacccacaac tcaaaggaaa aggacatgta attatcattg actagcccat
      841 ctcaattggt atagtgatta aaatcaccta gaccaattga gatgtatgtc tgaattagtt
      901 gttttcaaag caaatgaact agcgattagt cgctatgact taacggagca tgaaaccaag
      961 ctaattttat gctgtgtggc actactcaac cccacgattg aaaaccctac aaggaaagaa
     1021 cggacggtat cgttcactta taaccaatac gctcagatga tgaacatcag tagggaaaat
     1081 gcttatggtg tattagctaa agcaaccaga gagctgatga cgagaactgt ggaaatcagg
     1141 aatcctttgg ttaaaggctt tgagattttc cagtggacaa actatgccaa gttctcaagc
     1201 gaaaaattag aattagtttt tagtgaagag atattgcctt atcttttcca gttaaaaaaa
     1261 ttcataaaat ataatctgga acatgttaag tcttttgaaa acaaatactc tatgaggatt
     1321 tatgagtggt tattaaaaga actaacacaa aagaaaactc acaaggcaaa tatagagatt
     1381 agccttgatg aatttaagtt catgttaatg cttgaaaata actaccatga gtttaaaagg
     1441 cttaaccaat gggttttgaa accaataagt aaagatttaa acacttacag caatatgaaa
     1501 ttggtggttg ataagcgagg ccgcccgact gatacgttga ttttccaagt tgaactagat
     1561 agacaaatgg atctcgtaac cgaacttgag aacaaccaga taaaaatgaa tggtgacaaa
     1621 ataccaacaa ccattacatc agattcctac ctacataacg gactaagaaa aacactacac
     1681 gatgctttaa ctgcaaaaat tcagctcacc agttttgagg caaaattttt gagtgacatg
     1741 caaagtaagt atgatctcaa tggttcgttc tcatggctca cgcaaaaaca acgaaccaca
     1801 ctagagaaca tactggctaa atacggaagg atctgaggtt cttatggctc ttgtatctat
     1861 cagtgaagca tcaagactaa caaacaaaag tagaacaact gttcaccgtt acatatcaaa
     1921 gggaaaactg tccatatgca cagatgaaaa cggtgtaaaa aagatagata catcagagct
     1981 tttacgagtt tttggtgcat tcaaagctgt tcaccatgaa cagatcgaca atgtaac
//