BBa_I50042 1 pSC101 pSC101 origin of replication 2007-04-10T11:00:00Z 2015-08-31T04:07:42Z This part was amplified from pSB4A3-P1010. pSC101 origin of replication which is supposed to have low copy number. This was the origin used in Michael Elowitz's repressilator and in Bujard's pZS4Int-1 vector with mutations introduced to eliminate a SpeI site. It is also the origin of replication in pSB4* series plasmids. false false _41_ 0 126 70 Not in stock false The replication origin boundaries were chosen quite liberally (i.e. they might include more sequence than is strictly necessary) because a different version of this origin (BBa_I50040) was shown to be non-functional. false Reshma Shetty annotation2071324 1 -10 range2071324 1 850 855 annotation2071309 1 Terminator range2071309 1 46 75 annotation2071332 1 G to C difference with canonical pSC101 vector sequence range2071332 1 1912 1912 annotation2071328 1 repA range2071328 1 886 1836 annotation2071334 1 inverted repeat range2071334 1 1963 1979 annotation2071312 1 Tn3 integration site range2071312 1 158 162 annotation2071318 1 direct repeat range2071318 1 737 756 annotation2071323 1 promoter region range2071323 1 846 865 annotation2071322 1 inverted repeat range2071322 1 838 859 annotation2071335 1 T to C difference with canonical pSC101 vector sequence range2071335 1 2002 2002 annotation2071325 1 inverted repeat range2071325 1 862 884 annotation2071331 1 inverted repeat range2071331 1 1847 1861 annotation2071311 1 inverted repeat range2071311 1 139 152 annotation2071321 1 -35 range2071321 1 828 833 annotation2071317 1 direct repeat range2071317 1 716 735 annotation2071315 1 inverted repeat range2071315 1 221 238 annotation2071313 1 inverted repeat range2071313 1 165 181 annotation2071327 1 RBS range2071327 1 879 884 annotation2071319 1 direct repeat range2071319 1 769 788 annotation2071320 1 mutated SpeI site range2071320 1 814 819 annotation2071316 1 AT rich region range2071316 1 635 717 annotation2071326 1 RBS range2071326 1 870 882 annotation2071329 1 G to A difference with canonical pSC101 vector sequence range2071329 1 1655 1655 annotation2071314 1 inverted repeat range2071314 1 192 215 annotation2071330 1 C to T difference with canonical pSC101 vector sequence range2071330 1 1750 1750 annotation2071310 1 par region range2071310 1 137 504 annotation2071333 1 putative polyA range2071333 1 1947 1962 BBa_I50042_sequence 1 ctgtcagaccaagtttacgagctcgcttggactcctgttgatagatccagtaatgacctcagaactccatctggatttgttcagaacgctcggttgccgccgggcgttttttattggtgagaatccaagcactagggacagtaagacgggtaagcctgttgatgataccgctgccttactgggtgcattagccagtctgaatgacctgtcacgggataatccgaagtggtcagactggaaaatcagagggcaggaactgctgaacagcaaaaagtcagatagcaccacatagcagacccgccataaaacgccctgagaagcccgtgacgggcttttcttgtattatgggtagtttccttgcatgaatccataaaaggcgcctgtagtgccatttacccccattcactgccagagccgtgagcgcagcgaactgaatgtcacgaaaaagacagcgactcaggtgcctgatggtcggagacaaaaggaatattcagcgatttgcccgagcttgcgagggtgctacttaagcctttagggttttaaggtctgttttgtagaggagcaaacagcgtttgcgacatccttttgtaatactgcggaactgactaaagtagtgagttatacacagggctgggatctattctttttatctttttttattctttctttattctataaattataaccacttgaatataaacaaaaaaaacacacaaaggtctagcggaatttacagagggtctagcagaatttacaagttttccagcaaaggtctagcagaatttacagatacccacaactcaaaggaaaaggacatgtaattatcattgactagcccatctcaattggtatagtgattaaaatcacctagaccaattgagatgtatgtctgaattagttgttttcaaagcaaatgaactagcgattagtcgctatgacttaacggagcatgaaaccaagctaattttatgctgtgtggcactactcaaccccacgattgaaaaccctacaaggaaagaacggacggtatcgttcacttataaccaatacgctcagatgatgaacatcagtagggaaaatgcttatggtgtattagctaaagcaaccagagagctgatgacgagaactgtggaaatcaggaatcctttggttaaaggctttgagattttccagtggacaaactatgccaagttctcaagcgaaaaattagaattagtttttagtgaagagatattgccttatcttttccagttaaaaaaattcataaaatataatctggaacatgttaagtcttttgaaaacaaatactctatgaggatttatgagtggttattaaaagaactaacacaaaagaaaactcacaaggcaaatatagagattagccttgatgaatttaagttcatgttaatgcttgaaaataactaccatgagtttaaaaggcttaaccaatgggttttgaaaccaataagtaaagatttaaacacttacagcaatatgaaattggtggttgataagcgaggccgcccgactgatacgttgattttccaagttgaactagatagacaaatggatctcgtaaccgaacttgagaacaaccagataaaaatgaatggtgacaaaataccaacaaccattacatcagattcctacctacataacggactaagaaaaacactacacgatgctttaactgcaaaaattcagctcaccagttttgaggcaaaatttttgagtgacatgcaaagtaagtatgatctcaatggttcgttctcatggctcacgcaaaaacaacgaaccacactagagaacatactggctaaatacggaaggatctgaggttcttatggctcttgtatctatcagtgaagcatcaagactaacaaacaaaagtagaacaactgttcaccgttacatatcaaagggaaaactgtccatatgcacagatgaaaacggtgtaaaaaagatagatacatcagagcttttacgagtttttggtgcattcaaagctgttcaccatgaacagatcgacaatgtaac igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z