LOCUS       BBa_I5020                878 bp    DNA     linear       03-May-2024
DEFINITION  Olmecs: Inducible expression of 434 cI repressor
ACCESSION   BBa_I5020
VERSION     BBa_I5020.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     CDS             82..750
                     /label=cI 434
     misc_feature    712..750
                     /label=LVA
     misc_feature    82..750
                     /label=BBa_C0052
     misc_feature    751..775
                     /label=Help:Barcodes
     CDS             82..750
                     /label=BBa_C0052
     stem_loop       791..810
                     /label=T7 TE
     misc_feature    817..817
                     /label=stop
     polyA_site      811..824
                     /label=polya
     misc_feature    784..824
                     /label=BBa_B0012
     terminator      784..824
                     /label=BBa_B0012
     stem_loop       845..867
                     /label=stem_loop
     misc_feature    833..878
                     /label=BBa_B0011
     terminator      833..878
                     /label=BBa_B0011
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagaaagagg agaaatacta gatgagtatt tcttccaggg taaaaagcaa aagaatccag
      121 cttggactta accaggctga acttgctcaa aaggtgggga ctacccagca gtctatagag
      181 cagctcgaaa acggtaaaac taagcgacca cgctttttac cagaacttgc gtcagctctt
      241 ggcgtaagtg ttgactggct gctcaatggc acctctgatt cgaatgttag atttgttggg
      301 cacgttgagc ccaaagggaa atatccattg attagcatgg ttagagctgg ttcgtggtgt
      361 gaagcttgtg aaccctacga tatcaaggac attgatgaat ggtatgacag tgacgttaac
      421 ttattaggca atggattctg gctgaaggtt gaaggtgatt ccatgacctc acctgtaggt
      481 caaagcatcc ctgaaggtca tatggtgtta gtagatactg gacgggagcc agtgaatgga
      541 agccttgttg tagccaaact gactgacgcg aacgaagcaa cattcaagaa actggtcata
      601 gatggcggtc agaagtacct gaaaggcctg aatccttcat ggcctatgac tcctatcaac
      661 ggaaactgca agattatcgg tgttgtcgtg gaagcgaggg taaaattcgt agctgcaaac
      721 gacgaaaact acgctttagt agcttaataa ctctgatagt gctagtgtag atctctacta
      781 gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tatatactag agagagaata
      841 taaaaagcca gattattaat ccggcttttt tattattt
//