LOCUS BBa_I51019 4876 bp DNA linear 09-May-2024 DEFINITION BioBrick base vector, as synthesized by Codon Devices ACCESSION BBa_I51019 VERSION BBa_I51019.1 FEATURES Location/Qualifiers misc_feature 1..21 /label=Suffix misc_feature 2..7 /label=SpeI misc_feature 16..21 /label=PstI misc_feature 9..16 /label=NotI misc_feature 1..21 /label=BBa_G00001 misc_feature 22..26 /label=Nt.BstNB I recognition site misc_feature 27..29 /label=Arbitrary sequence (could be any bases) misc_feature 30..34 /label=Topoisomerase I recognition site misc_feature 30..30 /label=Generated T overhang misc_feature 22..34 /label=BBa_B0044 misc_feature 36..38 /label=stop codon (+2 frame) misc_feature 35..37 /label=stop codon (-1 frame) misc_feature 40..42 /label=stop codon (+3 frame) misc_feature 44..46 /label=stop codon (+1 frame) misc_feature 43..45 /label=stop codon (-2 frame) misc_feature 39..41 /label=stop codon (-3 frame) misc_feature 35..46 /label=BBa_B0042 stem_loop 57..88 /label=stem loop misc_feature 50..89 /label=identical to E. coli MG1655 between tonB and yciA terminator 47..115 /label=BBa_B0054 primer_bind 116..135 /label=VR misc_feature 116..135 /label=BBa_G00102 stem_loop 149..168 /label=stem_loop terminator 136..176 /label=BBa_B0062 misc_feature 177..182 /label=NheI site misc_feature 177..182 /label=BBa_B0045 CDS 183..1046 /label=ampicillin resistance RBS 1051..1055 /label=ampR RBS promoter 1064..1069 /label=ampR -10 promoter 1090..1095 /label=ampR -35 misc_feature 183..1125 /label=BBa_P1006 misc_feature 1126..1131 /label=NheI site misc_feature 1126..1131 /label=BBa_B0045 stem_loop 1140..1174 /label=stem_loop terminator 1132..1203 /label=BBa_B0053 primer_bind 1204..1223 /label=VF2 primer 1204..1223 /label=BBa_G00100 stem_loop 1259..1280 /label=stem loop from bacteriophage T3 misc_feature 1251..1289 /label=identical to bacteriophage T3 terminator 1224..1301 /label=BBa_B0055 misc_feature 1303..1305 /label=stop codon (+2 frame) misc_feature 1302..1304 /label=stop codon (-1 frame) misc_feature 1307..1309 /label=stop codon (+3 frame) misc_feature 1311..1313 /label=stop codon (+1 frame) misc_feature 1310..1312 /label=stop codon (-2 frame) misc_feature 1306..1308 /label=stop codon (-3 frame) misc_feature 1302..1313 /label=BBa_B0042 misc_feature 1314..1318 /label=Topoisomerase I recognition site misc_feature 1322..1326 /label=Nt.BstNB I recognition site misc_feature 1319..1321 /label=Arbitrary sequence (could be any bases) misc_feature 1318..1318 /label=Generated T overhang misc_feature 1314..1326 /label=BBa_B0043 misc_feature 1327..1332 /label=EcoRI site misc_feature 1342..1347 /label=XbaI site misc_feature 1327..1348 /label=Prefix misc_feature 1333..1340 /label=NotI site misc_feature 1327..1348 /label=BBa_G00000 promoter 1389..1394 /label=-35 promoter 1412..1417 /label=-10 CDS 1456..1764 /label=ccdB misc_feature 1349..1764 /label=BBa_P1016 misc_feature 1780..2394 /label=rep (pMB1) misc_feature 2379..2379 /label=ORI misc_feature 1791..2379 /label=origin of replication misc_feature 1827..2379 /label=RNAII transcript promoter 1968..1973 /label=RNAI transcript promoter -35 promoter 1946..1951 /label=RNAI transcript promoter -10 misc_feature 1830..1937 /label=RNAI transcript promoter 1812..1817 /label=RNAII transcript promoter -10 sequence promoter 1791..1796 /label=RNAII transcript promoter -35 sequence misc_feature 2025..2025 /label=G to A, nonfunctional mutation misc_feature 2029..2029 /label=G to A, nonfunctional mutation misc_feature 2038..2038 /label=G to A, nonfunctional mutation misc_feature 2067..2067 /label=C to T, nonfunctional mutation misc_feature 2231..2231 /label=C to T, nonfunctional mutation misc_feature 1765..2438 /label=BBa_I50020 misc_feature 2439..2459 /label=Suffix misc_feature 2440..2445 /label=SpeI misc_feature 2454..2459 /label=PstI misc_feature 2447..2454 /label=NotI misc_feature 2439..2459 /label=BBa_G00001 misc_feature 2460..2464 /label=Nt.BstNB I recognition site misc_feature 2465..2467 /label=Arbitrary sequence (could be any bases) misc_feature 2468..2472 /label=Topoisomerase I recognition site misc_feature 2468..2468 /label=Generated T overhang misc_feature 2460..2472 /label=BBa_B0044 misc_feature 2474..2476 /label=stop codon (+2 frame) misc_feature 2473..2475 /label=stop codon (-1 frame) misc_feature 2478..2480 /label=stop codon (+3 frame) misc_feature 2482..2484 /label=stop codon (+1 frame) misc_feature 2481..2483 /label=stop codon (-2 frame) misc_feature 2477..2479 /label=stop codon (-3 frame) misc_feature 2473..2484 /label=BBa_B0042 stem_loop 2495..2526 /label=stem loop misc_feature 2488..2527 /label=identical to E. coli MG1655 between tonB and yciA terminator 2485..2553 /label=BBa_B0054 primer_bind 2554..2573 /label=VR misc_feature 2554..2573 /label=BBa_G00102 stem_loop 2587..2606 /label=stem_loop terminator 2574..2614 /label=BBa_B0062 misc_feature 2615..2620 /label=NheI site misc_feature 2615..2620 /label=BBa_B0045 CDS 2621..3484 /label=ampicillin resistance RBS 3489..3493 /label=ampR RBS promoter 3502..3507 /label=ampR -10 promoter 3528..3533 /label=ampR -35 misc_feature 2621..3563 /label=BBa_P1006 misc_feature 3564..3569 /label=NheI site misc_feature 3564..3569 /label=BBa_B0045 stem_loop 3578..3612 /label=stem_loop terminator 3570..3641 /label=BBa_B0053 primer_bind 3642..3661 /label=VF2 primer 3642..3661 /label=BBa_G00100 stem_loop 3697..3718 /label=stem loop from bacteriophage T3 misc_feature 3689..3727 /label=identical to bacteriophage T3 terminator 3662..3739 /label=BBa_B0055 misc_feature 3741..3743 /label=stop codon (+2 frame) misc_feature 3740..3742 /label=stop codon (-1 frame) misc_feature 3745..3747 /label=stop codon (+3 frame) misc_feature 3749..3751 /label=stop codon (+1 frame) misc_feature 3748..3750 /label=stop codon (-2 frame) misc_feature 3744..3746 /label=stop codon (-3 frame) misc_feature 3740..3751 /label=BBa_B0042 misc_feature 3752..3756 /label=Topoisomerase I recognition site misc_feature 3760..3764 /label=Nt.BstNB I recognition site misc_feature 3757..3759 /label=Arbitrary sequence (could be any bases) misc_feature 3756..3756 /label=Generated T overhang misc_feature 3752..3764 /label=BBa_B0043 misc_feature 3765..3770 /label=EcoRI site misc_feature 3780..3785 /label=XbaI site misc_feature 3765..3786 /label=Prefix misc_feature 3771..3778 /label=NotI site misc_feature 3765..3786 /label=BBa_G00000 promoter 3827..3832 /label=-35 promoter 3850..3855 /label=-10 CDS 3894..4202 /label=ccdB misc_feature 3787..4202 /label=BBa_P1016 misc_feature 4218..4832 /label=rep (pMB1) promoter 4229..4234 /label=RNAII transcript promoter -35 sequence misc_feature 4229..4817 /label=origin of replication promoter 4250..4255 /label=RNAII transcript promoter -10 sequence misc_feature 4265..4817 /label=RNAII transcript misc_feature 4268..4375 /label=RNAI transcript promoter 4384..4389 /label=RNAI transcript promoter -10 promoter 4406..4411 /label=RNAI transcript promoter -35 misc_feature 4817..4817 /label=ORI misc_feature 4203..4876 /label=BBa_I50022 ORIGIN 1 tactagtagc ggccgctgca ggagtcacta agggttagtt agttagatta gcagaaagtc 61 aaaagcctcc gaccggaggc ttttgactaa aacttccctt ggggttatca ttggggctca 121 ctcaaaggcg gtaatcagat aaaaaaaatc cttagctttc gctaaggatg atttctgcta 181 gcttattacc aatgcttaat cagggaggca cctatctcag cgatctggcg attacgttcg 241 tccatagttg cctggctccc cgtcgtgtag ataactacga tacgggaggg cttaccatct 301 ggccccaggg ctgcaataat accgcgggac ccacgctcac cggctccaga tttatcagca 361 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc 421 atccagtcta ttaattgttg ccgggaagcg agagtaagaa gttcgccagt taagagtttg 481 cgcaacgttg ttgccatagc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct 541 tcattcagct ccggttccca acgatcaagg cgagttacat gatcacccat gttgtgcaaa 601 aaagcggtaa gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgccgtgtta 661 tcactcatgg ttatggcagc gctgcataat tcgcgtactg tcatgccgtc cgtaagatgc 721 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg 781 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacagagcag aactttaaaa 841 gtgctcatca ttgggaaacg ttcttcgggg cgaaaactct caagaatctt accgctgttg 901 aggtccagtt cgatgtaacc cacacgtgca cccaactgat cttcggcatc ttttactttc 961 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg 1021 gcgacacgga aatgttgaat actcatattc ttcctttttc aatattattg aagcatttat 1081 cagggttatt gtctcatgag cggatagata tttgaatgta tttaggctag ctccggcaaa 1141 aaaacgggca aggtgtcacc accctgccct ttttctttaa aaccgaaaag attacttcgc 1201 gtttgccacc tgacgtctaa gaaaaggaat attcagcaat ttgcccgtgc cgaagaaagg 1261 cccacccgtg aaggtgagcc agtgagttga ttgctacgta attagttagt tagcccttag 1321 tgactcgaat tcgcggccgc ttctagaggg cttactaaaa gccagataac agtatgcgta 1381 tttgcgcgct gatttttgcg gtataagaat atatactgat atgtataccc gaagtatgtc 1441 aaaaagaggt atgctatgca gtttaaggtt tacacctata aaagagagag ccgttatcgt 1501 ctgtttgtgg atgtacagag tgatattatt gacacgcccg ggcgacggat ggtgatcccc 1561 ctggccagtg cacgtctgct gtcagataaa gtctcccgtg aactttaccc ggtggtgcat 1621 atcggggatg aaagctggcg catgatgacc accgatatgg ccagtgtgcc ggtctccgtt 1681 atcggggaag aagtggctga tctcagccac cgcgaaaatg acatcaaaaa cgccattaac 1741 ctgatgttct ggggaatata ataacccgta gaaaagatca aaagatcttc ttgagatcct 1801 ttttttctgc gcgtaatctg ctacttgcaa acaaaaaaac caccgctacc agcggtggtt 1861 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt tagcagagcg 1921 cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct 1981 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtagctac tgccagtagc 2041 gataagtcgt gtcttaccgg gttggattca agacgatagt taccggataa ggcgcagcgg 2101 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa 2161 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg 2221 gacaggtatc tggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg 2281 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga 2341 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt 2401 ttacggttcc tggccttttg ctggcctttt gctcacatta ctagtagcgg ccgctgcagg 2461 agtcactaag ggttagttag ttagattagc agaaagtcaa aagcctccga ccggaggctt 2521 ttgactaaaa cttcccttgg ggttatcatt ggggctcact caaaggcggt aatcagataa 2581 aaaaaatcct tagctttcgc taaggatgat ttctgctagc ttattaccaa tgcttaatca 2641 gggaggcacc tatctcagcg atctggcgat tacgttcgtc catagttgcc tggctccccg 2701 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagggct gcaataatac 2761 cgcgggaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg 2821 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc 2881 gggaagcgag agtaagaagt tcgccagtta agagtttgcg caacgttgtt gccatagcta 2941 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac 3001 gatcaaggcg agttacatga tcacccatgt tgtgcaaaaa agcggtaagc tccttcggtc 3061 ctccgatcgt tgtcagaagt aagttggccg ccgtgttatc actcatggtt atggcagcgc 3121 tgcataattc gcgtactgtc atgccgtccg taagatgctt ttctgtgact ggtgagtact 3181 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa 3241 tacgggataa taccgcgcca cagagcagaa ctttaaaagt gctcatcatt gggaaacgtt 3301 cttcggggcg aaaactctca agaatcttac cgctgttgag gtccagttcg atgtaaccca 3361 cacgtgcacc caactgatct tcggcatctt ttactttcac cagcgtttct gggtgagcaa 3421 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac 3481 tcatattctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg 3541 gatagatatt tgaatgtatt taggctagct ccggcaaaaa aacgggcaag gtgtcaccac 3601 cctgcccttt ttctttaaaa ccgaaaagat tacttcgcgt ttgccacctg acgtctaaga 3661 aaaggaatat tcagcaattt gcccgtgccg aagaaaggcc cacccgtgaa ggtgagccag 3721 tgagttgatt gctacgtaat tagttagtta gcccttagtg actcgaattc gcggccgctt 3781 ctagagggct tactaaaagc cagataacag tatgcgtatt tgcgcgctga tttttgcggt 3841 ataagaatat atactgatat gtatacccga agtatgtcaa aaagaggtat gctatgcagt 3901 ttaaggttta cacctataaa agagagagcc gttatcgtct gtttgtggat gtacagagtg 3961 atattattga cacgcccggg cgacggatgg tgatccccct ggccagtgca cgtctgctgt 4021 cagataaagt ctcccgtgaa ctttacccgg tggtgcatat cggggatgaa agctggcgca 4081 tgatgaccac cgatatggcc agtgtgccgg tctccgttat cggggaagaa gtggctgatc 4141 tcagccaccg cgaaaatgac atcaaaaacg ccattaacct gatgttctgg ggaatataat 4201 aacccgtaga aaagatcaaa agatcttctt gagatccttt ttttctgcgc gtaatctgct 4261 acttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac 4321 caactctttt tccgaaggta actggcttta gcagagcgca gataccaaat actgtccttc 4381 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg 4441 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt 4501 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt 4561 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc 4621 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca 4681 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata 4741 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg 4801 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct 4861 ggccttttgc tcacat //