LOCUS       BBa_I52000              1098 bp    DNA     linear       29-Apr-2024
DEFINITION  ccdB and minimal pUC19 derived high copy origin
ACCESSION   BBa_I52000
VERSION     BBa_I52000.1  
FEATURES             Location/Qualifiers
     promoter        41..46
                     /label=-35
     promoter        64..69
                     /label=-10
     CDS             108..416
                     /label=ccdB
     misc_feature    1..416
                     /label=BBa_P1011
     misc_feature    440..1054
                     /label=rep (pMB1)
     misc_feature    1039..1039
                     /label=ORI
     misc_feature    451..1039
                     /label=origin of replication
     misc_feature    487..1039
                     /label=RNAII transcript
     promoter        628..633
                     /label=RNAI transcript promoter -35
     promoter        606..611
                     /label=RNAI transcript promoter -10
     misc_feature    490..597
                     /label=RNAI transcript
     promoter        472..477
                     /label=RNAII transcript promoter -10 sequence
     promoter        451..456
                     /label=RNAII transcript promoter -35 sequence
     misc_feature    685..685
                     /label=G to A, nonfunctional mutation
     misc_feature    689..689
                     /label=G to A, nonfunctional mutation
     misc_feature    698..698
                     /label=G to A, nonfunctional mutation
     misc_feature    727..727
                     /label=C to T, nonfunctional mutation
     misc_feature    891..891
                     /label=C to T, nonfunctional mutation
     misc_feature    425..1098
                     /label=BBa_I50020
ORIGIN
        1 ggcttactaa aagccagata acagtatgcg tatttgcgcg ctgatttttg cggtataaga
       61 atatatactg atatgtatac ccgaagtatg tcaaaaagag gtatgctatg cagtttaagg
      121 tttacaccta taaacgtgag agccgttatc gtctgtttgt ggacgtacag agtgatatta
      181 ttgacacgcc tgggcgtcgc atggtgattc ccctggccag cgcacgtctg ctttcagata
      241 aagtctcccg tgaactttac ccggtggtgc atatcgggga cgaaagctgg cgcatgatga
      301 ccaccgatat ggccagcgtg ccggtttccg tgatcgggga agaagtggct gatctcagcc
      361 accgcgaaaa tgacatcaaa aacgccatta acctgatgtt ctggggaata taataatact
      421 agagcccgta gaaaagatca aaagatcttc ttgagatcct ttttttctgc gcgtaatctg
      481 ctacttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
      541 accaactctt tttccgaagg taactggctt tagcagagcg cagataccaa atactgtcct
      601 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
      661 cgctctgcta atcctgttac cagtagctac tgccagtagc gataagtcgt gtcttaccgg
      721 gttggattca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
      781 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
      841 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc tggtaagcgg
      901 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
      961 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     1021 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     1081 ctggcctttt gctcacat
//