LOCUS BBa_I53423 3012 bp DNA linear 29-May-2024 DEFINITION pSC101.Kan ACCESSION BBa_I53423 VERSION BBa_I53423.1 FEATURES Location/Qualifiers stem_loop 46..75 /label=Terminator misc_feature 137..504 /label=par region misc_feature 139..152 /label=inverted repeat misc_feature 158..162 /label=Tn3 integration site misc_feature 165..181 /label=inverted repeat misc_feature 192..215 /label=inverted repeat misc_feature 221..238 /label=inverted repeat misc_feature 635..717 /label=AT rich region misc_feature 716..735 /label=direct repeat misc_feature 737..756 /label=direct repeat misc_feature 769..788 /label=direct repeat misc_feature 814..819 /label=mutated SpeI site promoter 828..833 /label=-35 misc_feature 838..859 /label=inverted repeat promoter 846..865 /label=promoter region promoter 850..855 /label=-10 misc_feature 862..884 /label=inverted repeat RBS 870..882 /label=RBS RBS 879..884 /label=RBS CDS 886..1836 /label=repA misc_feature 1655..1655 /label=G to A difference with canonical pSC101 vector sequence misc_feature 1750..1750 /label=C to T difference with canonical pSC101 vector sequence misc_feature 1847..1861 /label=inverted repeat misc_feature 1912..1912 /label=G to C difference with canonical pSC101 vector sequence polyA_site 1947..1962 /label=putative polyA misc_feature 1963..1979 /label=inverted repeat misc_feature 2002..2002 /label=T to C difference with canonical pSC101 vector sequence misc_feature 1..2037 /label=BBa_I50042 CDS 2194..3012 /label=kanamycin resistance misc_feature 2046..3012 /label=BBa_P1003 ORIGIN 1 ctgtcagacc aagtttacga gctcgcttgg actcctgttg atagatccag taatgacctc 61 agaactccat ctggatttgt tcagaacgct cggttgccgc cgggcgtttt ttattggtga 121 gaatccaagc actagggaca gtaagacggg taagcctgtt gatgataccg ctgccttact 181 gggtgcatta gccagtctga atgacctgtc acgggataat ccgaagtggt cagactggaa 241 aatcagaggg caggaactgc tgaacagcaa aaagtcagat agcaccacat agcagacccg 301 ccataaaacg ccctgagaag cccgtgacgg gcttttcttg tattatgggt agtttccttg 361 catgaatcca taaaaggcgc ctgtagtgcc atttaccccc attcactgcc agagccgtga 421 gcgcagcgaa ctgaatgtca cgaaaaagac agcgactcag gtgcctgatg gtcggagaca 481 aaaggaatat tcagcgattt gcccgagctt gcgagggtgc tacttaagcc tttagggttt 541 taaggtctgt tttgtagagg agcaaacagc gtttgcgaca tccttttgta atactgcgga 601 actgactaaa gtagtgagtt atacacaggg ctgggatcta ttctttttat ctttttttat 661 tctttcttta ttctataaat tataaccact tgaatataaa caaaaaaaac acacaaaggt 721 ctagcggaat ttacagaggg tctagcagaa tttacaagtt ttccagcaaa ggtctagcag 781 aatttacaga tacccacaac tcaaaggaaa aggacatgta attatcattg actagcccat 841 ctcaattggt atagtgatta aaatcaccta gaccaattga gatgtatgtc tgaattagtt 901 gttttcaaag caaatgaact agcgattagt cgctatgact taacggagca tgaaaccaag 961 ctaattttat gctgtgtggc actactcaac cccacgattg aaaaccctac aaggaaagaa 1021 cggacggtat cgttcactta taaccaatac gctcagatga tgaacatcag tagggaaaat 1081 gcttatggtg tattagctaa agcaaccaga gagctgatga cgagaactgt ggaaatcagg 1141 aatcctttgg ttaaaggctt tgagattttc cagtggacaa actatgccaa gttctcaagc 1201 gaaaaattag aattagtttt tagtgaagag atattgcctt atcttttcca gttaaaaaaa 1261 ttcataaaat ataatctgga acatgttaag tcttttgaaa acaaatactc tatgaggatt 1321 tatgagtggt tattaaaaga actaacacaa aagaaaactc acaaggcaaa tatagagatt 1381 agccttgatg aatttaagtt catgttaatg cttgaaaata actaccatga gtttaaaagg 1441 cttaaccaat gggttttgaa accaataagt aaagatttaa acacttacag caatatgaaa 1501 ttggtggttg ataagcgagg ccgcccgact gatacgttga ttttccaagt tgaactagat 1561 agacaaatgg atctcgtaac cgaacttgag aacaaccaga taaaaatgaa tggtgacaaa 1621 ataccaacaa ccattacatc agattcctac ctacataacg gactaagaaa aacactacac 1681 gatgctttaa ctgcaaaaat tcagctcacc agttttgagg caaaattttt gagtgacatg 1741 caaagtaagt atgatctcaa tggttcgttc tcatggctca cgcaaaaaca acgaaccaca 1801 ctagagaaca tactggctaa atacggaagg atctgaggtt cttatggctc ttgtatctat 1861 cagtgaagca tcaagactaa caaacaaaag tagaacaact gttcaccgtt acatatcaaa 1921 gggaaaactg tccatatgca cagatgaaaa cggtgtaaaa aagatagata catcagagct 1981 tttacgagtt tttggtgcat tcaaagctgt tcaccatgaa cagatcgaca atgtaactac 2041 tagagctgat ccttcaactc agcaaaagtt cgatttattc aacaaagcca cgttgtgtct 2101 caaaatctct gatgttacat tgcacaagat aaaaatatat catcatgaac aataaaactg 2161 tctgcttaca taaacagtaa tacaaggggt gttatgagcc atattcaacg ggaaacgtct 2221 tgctcccgtc cgcgcttaaa ctccaacatg gacgctgatt tatatgggta taaatgggct 2281 cgcgataatg tcgggcaatc aggtgcgaca atctatcgct tgtatgggaa gcccgatgcg 2341 ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca atgatgttac agatgagatg 2401 gtccgtctca actggctgac ggagtttatg cctctcccga ccatcaagca ttttatccgt 2461 actcctgatg atgcgtggtt actcaccacc gcgattcctg ggaaaacagc cttccaggta 2521 ttagaagaat atcctgattc aggtgaaaat attgttgatg cgctggccgt gttcctgcgc 2581 cggttacatt cgattcctgt ttgtaattgt ccttttaaca gcgatcgtgt atttcgtctt 2641 gctcaggcgc aatcacgcat gaataacggt ttggttgatg cgagtgattt tgatgacgag 2701 cgtaatggct ggcctgttga acaagtctgg aaagaaatgc acaagctctt gccattctca 2761 ccggattcag tcgtcactca tggtgatttc tcacttgata accttatttt tgacgagggg 2821 aaattaatag gttgtattga tgttggacgg gtcggaatcg cagaccgtta ccaggacctt 2881 gccattcttt ggaactgcct cggtgagttt tctccttcat tacagaaacg gctttttcaa 2941 aaatatggta ttgataatcc tgatatgaat aaattgcagt ttcatttgat gctcgatgag 3001 tttttctaat aa //