LOCUS BBa_I53425 3328 bp DNA linear 19-May-2024 DEFINITION pSC101.Tet ACCESSION BBa_I53425 VERSION BBa_I53425.1 FEATURES Location/Qualifiers stem_loop 46..75 /label=Terminator misc_feature 137..504 /label=par region misc_feature 139..152 /label=inverted repeat misc_feature 158..162 /label=Tn3 integration site misc_feature 165..181 /label=inverted repeat misc_feature 192..215 /label=inverted repeat misc_feature 221..238 /label=inverted repeat misc_feature 635..717 /label=AT rich region misc_feature 716..735 /label=direct repeat misc_feature 737..756 /label=direct repeat misc_feature 769..788 /label=direct repeat misc_feature 814..819 /label=mutated SpeI site promoter 828..833 /label=-35 misc_feature 838..859 /label=inverted repeat promoter 846..865 /label=promoter region promoter 850..855 /label=-10 misc_feature 862..884 /label=inverted repeat RBS 870..882 /label=RBS RBS 879..884 /label=RBS CDS 886..1836 /label=repA misc_feature 1655..1655 /label=G to A difference with canonical pSC101 vector sequence misc_feature 1750..1750 /label=C to T difference with canonical pSC101 vector sequence misc_feature 1847..1861 /label=inverted repeat misc_feature 1912..1912 /label=G to C difference with canonical pSC101 vector sequence polyA_site 1947..1962 /label=putative polyA misc_feature 1963..1979 /label=inverted repeat misc_feature 2002..2002 /label=T to C difference with canonical pSC101 vector sequence misc_feature 1..2037 /label=BBa_I50042 promoter 2046..2134 /label=tetR promoter CDS 2135..3328 /label=tetA misc_feature 2046..3328 /label=BBa_P1005 ORIGIN 1 ctgtcagacc aagtttacga gctcgcttgg actcctgttg atagatccag taatgacctc 61 agaactccat ctggatttgt tcagaacgct cggttgccgc cgggcgtttt ttattggtga 121 gaatccaagc actagggaca gtaagacggg taagcctgtt gatgataccg ctgccttact 181 gggtgcatta gccagtctga atgacctgtc acgggataat ccgaagtggt cagactggaa 241 aatcagaggg caggaactgc tgaacagcaa aaagtcagat agcaccacat agcagacccg 301 ccataaaacg ccctgagaag cccgtgacgg gcttttcttg tattatgggt agtttccttg 361 catgaatcca taaaaggcgc ctgtagtgcc atttaccccc attcactgcc agagccgtga 421 gcgcagcgaa ctgaatgtca cgaaaaagac agcgactcag gtgcctgatg gtcggagaca 481 aaaggaatat tcagcgattt gcccgagctt gcgagggtgc tacttaagcc tttagggttt 541 taaggtctgt tttgtagagg agcaaacagc gtttgcgaca tccttttgta atactgcgga 601 actgactaaa gtagtgagtt atacacaggg ctgggatcta ttctttttat ctttttttat 661 tctttcttta ttctataaat tataaccact tgaatataaa caaaaaaaac acacaaaggt 721 ctagcggaat ttacagaggg tctagcagaa tttacaagtt ttccagcaaa ggtctagcag 781 aatttacaga tacccacaac tcaaaggaaa aggacatgta attatcattg actagcccat 841 ctcaattggt atagtgatta aaatcaccta gaccaattga gatgtatgtc tgaattagtt 901 gttttcaaag caaatgaact agcgattagt cgctatgact taacggagca tgaaaccaag 961 ctaattttat gctgtgtggc actactcaac cccacgattg aaaaccctac aaggaaagaa 1021 cggacggtat cgttcactta taaccaatac gctcagatga tgaacatcag tagggaaaat 1081 gcttatggtg tattagctaa agcaaccaga gagctgatga cgagaactgt ggaaatcagg 1141 aatcctttgg ttaaaggctt tgagattttc cagtggacaa actatgccaa gttctcaagc 1201 gaaaaattag aattagtttt tagtgaagag atattgcctt atcttttcca gttaaaaaaa 1261 ttcataaaat ataatctgga acatgttaag tcttttgaaa acaaatactc tatgaggatt 1321 tatgagtggt tattaaaaga actaacacaa aagaaaactc acaaggcaaa tatagagatt 1381 agccttgatg aatttaagtt catgttaatg cttgaaaata actaccatga gtttaaaagg 1441 cttaaccaat gggttttgaa accaataagt aaagatttaa acacttacag caatatgaaa 1501 ttggtggttg ataagcgagg ccgcccgact gatacgttga ttttccaagt tgaactagat 1561 agacaaatgg atctcgtaac cgaacttgag aacaaccaga taaaaatgaa tggtgacaaa 1621 ataccaacaa ccattacatc agattcctac ctacataacg gactaagaaa aacactacac 1681 gatgctttaa ctgcaaaaat tcagctcacc agttttgagg caaaattttt gagtgacatg 1741 caaagtaagt atgatctcaa tggttcgttc tcatggctca cgcaaaaaca acgaaccaca 1801 ctagagaaca tactggctaa atacggaagg atctgaggtt cttatggctc ttgtatctat 1861 cagtgaagca tcaagactaa caaacaaaag tagaacaact gttcaccgtt acatatcaaa 1921 gggaaaactg tccatatgca cagatgaaaa cggtgtaaaa aagatagata catcagagct 1981 tttacgagtt tttggtgcat tcaaagctgt tcaccatgaa cagatcgaca atgtaactac 2041 tagaggagat tctcatgttt gacagcttat catcgataag ctttaatgcg gtagtttatc 2101 acagttaaat tgctaacgca gtcaggcacc gtgtatgaaa tctaacaatg cgctcatcgt 2161 cattctcggc accgtcaccc tggacgctgt aggcataggc ttggttatgc cggtactgcc 2221 gggcctcttg cgggatatcg tccattccga cagtattgcc agtcactatg gcgtgctgct 2281 tgcgctctat gcgttgatgc aatttctttg cgcacccgtt ctcggagccc tgtccgaccg 2341 ctttggccgc cgtccagtcc tgctcgcttc gctccttgga gccactatcg actacgcgat 2401 catggcgacc acacccgtcc tgtggattct ctacgccgga cgcatcgtgg cgggcatcac 2461 gggtgccaca ggtgcggttg ctggtgccta tatcgccgac atcaccgatg gggaagatcg 2521 ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg ggtatggtgg caggccccgt 2581 ggccggggga ctgttgggtg ccatctcctt gcatgcacca ttccttgcgg cggcggtgct 2641 caacggcctc aacctcctcc tgggctgctt ccttatgcag gaatcgcata agggagagcg 2701 ccgtccgatg cccttgcgtg ccttcaatcc agtcagctcc ttccggtggg cgcggggcat 2761 gactatcgtc gccgcactta tgactgtttt ctttatcatg caactcgtag gacaggttcc 2821 ggcagcgctc tgggtcattt tcggcgagga ccgctttcgc tggagcgcga cgatgatcgg 2881 cctgtcgctt gcggtattcg gaatcttgca cgccctcgct caagccttcg tcacgggccc 2941 cgccaccaaa cgtttcggcg agaagcaggc cattatcgcg ggcatggcgg ccgacgcgct 3001 gggctacgtc ttgctggcgt tcgcgacgcg cggctggatg gccttcccca ttatgattct 3061 tctcgcttcc ggcggcatcg gtatgcccgc gttgcaggcc atgctgtccc gccaagtaga 3121 tgacgaccat cagggacagc ttcaagggtc gctcgcggct cttaccagcc tcacttcgat 3181 cattggaccg ctgatcgtca cggcgattta tgccgcctcg gcgagcacat ggaacgggtt 3241 ggcatggatt gtaggtgccg ccctttacct tgtctgcctc cccgcgttgc gtcgcggtgc 3301 atggagccgg gccacctcga cctaataa //