LOCUS       BBa_I6074               1076 bp    DNA     linear       17-May-2024
DEFINITION  Promoter O_H Test R0063.E0432
ACCESSION   BBa_I6074
VERSION     BBa_I6074.1  
FEATURES             Location/Qualifiers
     misc_feature    1..20
                     /label=LuxR/HSL
     promoter        115..122
                     /label=-10
     promoter        89..94
                     /label=-35
     misc_feature    128..128
                     /label=start
     misc_feature    130..149
                     /label=Putative LuxR/HSL
     misc_feature    1..151
                     /label=BBa_R0063
     promoter        1..151
                     /label=BBa_R0063
     misc_feature    164..167
                     /label=conserved
     RBS             160..171
                     /label=BBa_B0034
     CDS             178..939
                     /label=YFP (LVA)
     misc_feature    934..939
                     /label=2
     misc_feature    895..933
                     /label=SsrA
     misc_feature    419..419
                     /label=A (Q->L)
     misc_feature    178..939
                     /label=BBa_E0032
     misc_feature    178..939
                     /label=BBa_E0032
     stem_loop       959..1002
                     /label=stem_loop
     misc_feature    948..1027
                     /label=BBa_B0010
     terminator      948..1027
                     /label=BBa_B0010
     stem_loop       1043..1062
                     /label=T7 TE
     misc_feature    1069..1069
                     /label=stop
     polyA_site      1063..1076
                     /label=polya
     misc_feature    1036..1076
                     /label=BBa_B0012
     terminator      1036..1076
                     /label=BBa_B0012
ORIGIN
        1 acctgtacga tcctacaggt gcttatgtta agtaattgta ttcccagcga tacaatagtg
       61 tgacaaaaat ccaatttatt agaatcaaat gtcaatccat taccgtttta atgatatata
      121 acacgcaaaa cttgcgacaa acaataggta atactagaga aagaggagaa atactagatg
      181 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc
      241 gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc
      301 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc
      361 gtgaccacct tcggctacgg cctgcaatgc ttcgcccgct accccgacca catgaagctg
      421 cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc
      481 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg
      541 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag
      601 ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc
      661 atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac
      721 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac
      781 ctgagctacc agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg
      841 ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagaggcct
      901 gctgcaaacg acgaaaacta cgctttagta gcttaataat actagagcca ggcatcaaat
      961 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa
     1021 cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttata
//