LOCUS       BBa_I7010               1784 bp    DNA     linear       05-May-2024
DEFINITION  R0010.E0021.B0010.E0031
ACCESSION   BBa_I7010
VERSION     BBa_I7010.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     RBS             209..227
                     /label=Clontech RBS
     CDS             228..944
                     /label=ECFP
     misc_feature    945..947
                     /label=stop
     misc_feature    209..948
                     /label=BBa_E0021
     stem_loop       968..1011
                     /label=stem_loop
     misc_feature    957..1036
                     /label=BBa_B0010
     terminator      957..1036
                     /label=BBa_B0010
     RBS             1046..1064
                     /label=Clontech RBS
     CDS             1065..1780
                     /label=EYFP
     misc_feature    1781..1783
                     /label=stop
     misc_feature    1045..1784
                     /label=BBa_E0031
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagga aagaggagaa attaagcatg gtgagcaagg
      241 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
      301 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
      361 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc
      421 tgacctgggg cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct
      481 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
      541 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
      601 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
      661 actacatcag ccacaacgtc tatatcaccg ccgacaagca gaagaacggc atcaaggcca
      721 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc
      781 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc
      841 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
      901 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaatta ctagagccag
      961 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt
     1021 gtcggtgaac gctctctact agaggaaaga ggagaaatta agcatggtga gcaagggcga
     1081 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
     1141 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
     1201 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccttcgg
     1261 ctacggcctg caatgcttcg cccgctaccc cgaccacatg aagcagcacg acttcttcaa
     1321 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
     1381 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
     1441 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
     1501 caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt
     1561 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa
     1621 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gctaccagtc
     1681 cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
     1741 cgccgccggg atcactctcg gcatggacga gctgtacaag taat
//