LOCUS       BBa_I7013               1751 bp    DNA     linear       02-May-2024
DEFINITION  R0010.E0021.B0013.E0031
ACCESSION   BBa_I7013
VERSION     BBa_I7013.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     RBS             209..227
                     /label=Clontech RBS
     CDS             228..944
                     /label=ECFP
     misc_feature    945..947
                     /label=stop
     misc_feature    209..948
                     /label=BBa_E0021
     stem_loop       970..989
                     /label=T7 TE
     misc_feature    996..996
                     /label=stop
     misc_feature    993..993
                     /label=C
     misc_feature    966..966
                     /label=C
     polyA_site      957..962
                     /label=''
     polyA_site      990..1003
                     /label=polya
     misc_feature    957..1003
                     /label=BBa_B0013
     terminator      957..1003
                     /label=BBa_B0013
     RBS             1013..1031
                     /label=Clontech RBS
     CDS             1032..1747
                     /label=EYFP
     misc_feature    1748..1750
                     /label=stop
     misc_feature    1012..1751
                     /label=BBa_E0031
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagga aagaggagaa attaagcatg gtgagcaagg
      241 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
      301 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
      361 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc
      421 tgacctgggg cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct
      481 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
      541 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
      601 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
      661 actacatcag ccacaacgtc tatatcaccg ccgacaagca gaagaacggc atcaaggcca
      721 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc
      781 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc
      841 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
      901 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaatta ctagagaaaa
      961 aatcaaactg gctcaccttc gggtgggcct ttttgcgttt atatactaga ggaaagagga
     1021 gaaattaagc atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt
     1081 cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga
     1141 tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc
     1201 ctggcccacc ctcgtgacca ccttcggcta cggcctgcaa tgcttcgccc gctaccccga
     1261 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg
     1321 caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg
     1381 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat
     1441 cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa
     1501 gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt
     1561 gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc
     1621 cgacaaccac tacctgagct accagtccgc cctgagcaaa gaccccaacg agaagcgcga
     1681 tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct
     1741 gtacaagtaa t
//