LOCUS       BBa_I7015               1833 bp    DNA     linear       02-May-2024
DEFINITION  R0010.E0021.B0015.E0031
ACCESSION   BBa_I7015
VERSION     BBa_I7015.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     RBS             209..227
                     /label=Clontech RBS
     CDS             228..944
                     /label=ECFP
     misc_feature    945..947
                     /label=stop
     misc_feature    209..948
                     /label=BBa_E0021
     stem_loop       968..1011
                     /label=stem_loop
     misc_feature    957..1036
                     /label=BBa_B0010
     terminator      957..1036
                     /label=BBa_B0010
     stem_loop       1052..1071
                     /label=T7 TE
     misc_feature    1078..1078
                     /label=stop
     polyA_site      1072..1085
                     /label=polya
     misc_feature    1045..1085
                     /label=BBa_B0012
     terminator      1045..1085
                     /label=BBa_B0012
     RBS             1095..1113
                     /label=Clontech RBS
     CDS             1114..1829
                     /label=EYFP
     misc_feature    1830..1832
                     /label=stop
     misc_feature    1094..1833
                     /label=BBa_E0031
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagga aagaggagaa attaagcatg gtgagcaagg
      241 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
      301 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
      361 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc
      421 tgacctgggg cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct
      481 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
      541 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
      601 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
      661 actacatcag ccacaacgtc tatatcaccg ccgacaagca gaagaacggc atcaaggcca
      721 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc
      781 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc
      841 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
      901 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaatta ctagagccag
      961 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt
     1021 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt
     1081 ttatatacta gaggaaagag gagaaattaa gcatggtgag caagggcgag gagctgttca
     1141 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
     1201 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
     1261 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccttcggc tacggcctgc
     1321 aatgcttcgc ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
     1381 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
     1441 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
     1501 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca
     1561 acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc
     1621 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg
     1681 gcgacggccc cgtgctgctg cccgacaacc actacctgag ctaccagtcc gccctgagca
     1741 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga
     1801 tcactctcgg catggacgag ctgtacaagt aat
//