LOCUS       BBa_I7020               1786 bp    DNA     linear       06-May-2024
DEFINITION  R0010.E0021.B0020.E0031
ACCESSION   BBa_I7020
VERSION     BBa_I7020.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     RBS             209..227
                     /label=Clontech RBS
     CDS             228..944
                     /label=ECFP
     misc_feature    945..947
                     /label=stop
     misc_feature    209..948
                     /label=BBa_E0021
     stem_loop       983..1026
                     /label=stem_loop
     terminator      957..1038
                     /label=BBa_B0020
     RBS             1048..1066
                     /label=Clontech RBS
     CDS             1067..1782
                     /label=EYFP
     misc_feature    1783..1785
                     /label=stop
     misc_feature    1047..1786
                     /label=BBa_E0031
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagga aagaggagaa attaagcatg gtgagcaagg
      241 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
      301 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
      361 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc
      421 tgacctgggg cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct
      481 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
      541 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
      601 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
      661 actacatcag ccacaacgtc tatatcaccg ccgacaagca gaagaacggc atcaaggcca
      721 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc
      781 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc
      841 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
      901 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaatta ctagagggag
      961 agcgttcacc gacaaacaac agataaaacg aaaggcccag tctttcgact gagcctttcg
     1021 ttttatttga tgcctggtta ctagaggaaa gaggagaaat taagcatggt gagcaagggc
     1081 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
     1141 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
     1201 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccttc
     1261 ggctacggcc tgcaatgctt cgcccgctac cccgaccaca tgaagcagca cgacttcttc
     1321 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
     1381 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
     1441 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
     1501 tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac
     1561 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag
     1621 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagctaccag
     1681 tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     1741 accgccgccg ggatcactct cggcatggac gagctgtaca agtaat
//