BBa_B0014
1
BBa_B0014
double terminator (B0012-B0011)
2003-07-15T11:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Double terminator consisting of BBa_B0012 and BBa_B0011
false
true
_1_
0
24
7
In stock
false
true
Reshma Shetty
component939311
1
BBa_B0011
component939303
1
BBa_B0012
annotation939303
1
BBa_B0012
range939303
1
1
41
annotation939311
1
BBa_B0011
range939311
1
50
95
BBa_B0030
1
BBa_B0030
RBS.1 (strong) -- modified from R. Weiss
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_44_46_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation7025
1
BBa_B0030
range7025
1
1
15
annotation1702
1
RBS
range1702
1
8
12
annotation1701
1
RBS-1\Strong
range1701
1
1
15
BBa_I711021
1
BBa_I711021
Butanol dehydrogenase generator, beta-butryaldehyde -> butanol
2007-07-15T11:00:00Z
2015-08-31T04:07:45Z
Clostridium acetobutylicum ATCC 824
This part constitutively translates the aad gene, which produces and alcohol dehydrogenase that may be important in the synthesis of butanol from beta-butryaldehyde.
false
false
_158_
0
1498
9
Not in stock
false
None.
false
Ranjan Khan
component2222026
1
BBa_I711010
component2222033
1
BBa_B0014
component2222024
1
BBa_B0030
component2222022
1
BBa_J23119
annotation2222026
1
BBa_I711010
range2222026
1
65
1252
annotation2222024
1
BBa_B0030
range2222024
1
44
58
annotation2222033
1
BBa_B0014
range2222033
1
1261
1355
annotation2222022
1
BBa_J23119
range2222022
1
1
35
BBa_J23119
1
BBa_J23119
constitutive promoter family member
2006-08-23T11:00:00Z
2015-08-31T04:08:40Z
Overlap extension of synthetic oligonucleotides
Released HQ 2013
Later
false
true
_52_
0
483
95
In stock
false
N/A
true
John Anderson
BBa_I711010
1
BBa_I711010
Butanol dehydrogenase from Clostridium acetobutylicum ATCC 824
2007-07-11T11:00:00Z
2015-08-31T04:07:45Z
Clostridium acetobutylicum ATCC 824
This sequence codes for an alcohol dehydrogenase that is responsible for making butanol from butyraldehyde.
false
false
_158_
0
1498
9
Not in stock
false
None
false
Ranjan Khan
BBa_B0011
1
BBa_B0011
LuxICDABEG (+/-)
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from luxICDABEG operon terminator of Vibrio fischeri <genbank>AF170104</genbank>.
Released HQ 2013
Bidirectional transcriptional terminator consisting of a 22 bp stem-loop.</p>
false
false
_1_
0
24
7
In stock
false
<P> <P>In the naturally-occuring sequence there is a mismatch in the stem of the stem loop. This can be corrected via an A->G mutation (at position 40 -- sequence coordinate/not MFOLD coordinate). The above sequence does not reflect this mutation (but the MFOLD image does). This terminator's location cannot be found using some inverted repeat detectors like PALINDROME because it is too short and contains a mismatch. This one was found with the help of Tom Knight. It lies between two coding regions that point towards eachother.<P>
true
Reshma Shetty
annotation7019
1
BBa_B0011
range7019
1
1
46
annotation1683
1
stem_loop
range1683
1
13
35
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1690
1
polya
range1690
1
28
41
annotation1686
1
T7 TE
range1686
1
8
27
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1687
1
stop
range1687
1
34
34
BBa_B0014_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
BBa_I711021_sequence
1
ttgacagctagctcagtcctaggtataatgctagctactagagattaaagaggagaaatactagatgaaaaacacctactaccgcctgcaacagtacatgttcagcaaagcccagcgtctgctgccgtggcgtatgccaaacattatttccggctctaaatctatctgtaaactgccgtacaaagtgaaaaacgatggtgtaaaaaaagtgctggtgatcactactgcgggctctattaaacgcggctctctgaacgaaatgtttgatgaactgaagaaagaaaacatctcctacgtcatttatgatgatgttcaaccggatccgacgatcgaatgcatcgaagcagtagtgaaattctataagaaggaggcttgtgaaggtatcatcgccgtgggtggcggcagcgttatggattgcgcaaaagttactggcgctcgtatcgttaaaccaaaaaaaagcgttagccaaatggctggtctgatgaaaatccgtaaggagctgcctccgctgtacacggttccgactaccgccggtaccggttctgaaaccactgtcgcggctgtggttaccgattctagcacccatctgaaatatgcggtgtctgacctgtgcctgatccctaaatacgcggttctggatccggaactgacctgtggtctgccgaaaaacctgaccgcaatcactggtatggatgctctgactcacgcggttgaagcgtataccaataaatacagctctaaagaatcccgcaagtatgctctggacgccgtaaaactgatttttgagaacctgaaaaaggcatatgacaacggcaaacacattgaagtacgtgagaacatgctgaaagcatcttactatgccggcgtggcttttacccgctcctatgtgggttacgtacacgccatcgcccacgcggtcggtggcctgtacggtgtgcctcacggtatggccaacgctgtaatcctgcctgttgttctggaagcgtatggtgaagcgatctacaagaacctggcagaactggcggatgcaacgtccgtccctggtaaaacgcagatggaaaaagcgaaaaacttcattctgtctatccgtaaaatgaaccagagcatggaaattccggataagctggatagcatcaagaaagaggatatcccggaaatcatcaaccgtgcgatgaaagaggcaaacccgtcctacccggtaccggtaatttgggacaaaatccagttcgagaaagtggttgaatccctgaaatactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
BBa_B0030_sequence
1
attaaagaggagaaa
BBa_I711010_sequence
1
atgaaaaacacctactaccgcctgcaacagtacatgttcagcaaagcccagcgtctgctgccgtggcgtatgccaaacattatttccggctctaaatctatctgtaaactgccgtacaaagtgaaaaacgatggtgtaaaaaaagtgctggtgatcactactgcgggctctattaaacgcggctctctgaacgaaatgtttgatgaactgaagaaagaaaacatctcctacgtcatttatgatgatgttcaaccggatccgacgatcgaatgcatcgaagcagtagtgaaattctataagaaggaggcttgtgaaggtatcatcgccgtgggtggcggcagcgttatggattgcgcaaaagttactggcgctcgtatcgttaaaccaaaaaaaagcgttagccaaatggctggtctgatgaaaatccgtaaggagctgcctccgctgtacacggttccgactaccgccggtaccggttctgaaaccactgtcgcggctgtggttaccgattctagcacccatctgaaatatgcggtgtctgacctgtgcctgatccctaaatacgcggttctggatccggaactgacctgtggtctgccgaaaaacctgaccgcaatcactggtatggatgctctgactcacgcggttgaagcgtataccaataaatacagctctaaagaatcccgcaagtatgctctggacgccgtaaaactgatttttgagaacctgaaaaaggcatatgacaacggcaaacacattgaagtacgtgagaacatgctgaaagcatcttactatgccggcgtggcttttacccgctcctatgtgggttacgtacacgccatcgcccacgcggtcggtggcctgtacggtgtgcctcacggtatggccaacgctgtaatcctgcctgttgttctggaagcgtatggtgaagcgatctacaagaacctggcagaactggcggatgcaacgtccgtccctggtaaaacgcagatggaaaaagcgaaaaacttcattctgtctatccgtaaaatgaaccagagcatggaaattccggataagctggatagcatcaagaaagaggatatcccggaaatcatcaaccgtgcgatgaaagaggcaaacccgtcctacccggtaccggtaatttgggacaaaatccagttcgagaaagtggttgaatccctgaaa
BBa_B0011_sequence
1
agagaatataaaaagccagattattaatccggcttttttattattt
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_J23119_sequence
1
ttgacagctagctcagtcctaggtataatgctagc
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z