LOCUS       BBa_I7121               1031 bp    DNA     linear       13-May-2024
DEFINITION  R0040.B0032.E0040.M0100.B0015
ACCESSION   BBa_I7121
VERSION     BBa_I7121.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     RBS             63..75
                     /label=RBS-3\Weak
     RBS             69..72
                     /label=RBS
     misc_feature    63..75
                     /label=BBa_B0032
     RBS             63..75
                     /label=BBa_B0032
     CDS             82..801
                     /label=GFP protein
     CDS             82..801
                     /label=BBa_E0040
     misc_feature    810..894
                     /label=BBa_M0100
     stem_loop       914..957
                     /label=stem_loop
     misc_feature    903..982
                     /label=BBa_B0010
     terminator      903..982
                     /label=BBa_B0010
     stem_loop       998..1017
                     /label=T7 TE
     misc_feature    1024..1024
                     /label=stop
     polyA_site      1018..1031
                     /label=polya
     misc_feature    991..1031
                     /label=BBa_B0012
     terminator      991..1031
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agtcacacag gaaagtacta gatgcgtaaa ggagaagaac ttttcactgg agttgtccca
      121 attcttgttg aattagatgg tgatgttaat gggcacaaat tttctgtcag tggagagggt
      181 gaaggtgatg caacatacgg aaaacttacc cttaaattta tttgcactac tggaaaacta
      241 cctgttccat ggccaacact tgtcactact ttcggttatg gtgttcaatg ctttgcgaga
      301 tacccagatc atatgaaaca gcatgacttt ttcaagagtg ccatgcccga aggttatgta
      361 caggaaagaa ctatattttt caaagatgac gggaactaca agacacgtgc tgaagtcaag
      421 tttgaaggtg atacccttgt taatagaatc gagttaaaag gtattgattt taaagaagat
      481 ggaaacattc ttggacacaa attggaatac aactataact cacacaatgt atacatcatg
      541 gcagacaaac aaaagaatgg aatcaaagtt aacttcaaaa ttagacacaa cattgaagat
      601 ggaagcgttc aactagcaga ccattatcaa caaaatactc caattggcga tggccctgtc
      661 cttttaccag acaaccatta cctgtccaca caatctgccc tttcgaaaga tcccaacgaa
      721 aagagagacc acatggtcct tcttgagttt gtaacagctg ctgggattac acatggcatg
      781 gatgaactat acaaataata atactagagg atctgcattg tcctcttagt taaatggata
      841 taacgagccc ctcctaaggg ctaattgcag gttcgattcc tgcaggggac tccatactag
      901 agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg
      961 ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt
     1021 ctgcgtttat a
//