LOCUS       BBa_I715007             2846 bp    DNA     linear       20-May-2024
DEFINITION  hybrid pLac:RBS:Kan:RBS:Tet:RBS:RFP
ACCESSION   BBa_I715007
VERSION     BBa_I715007.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     CDS             82..897
                     /label=KanR Gene
     CDS             82..897
                     /label=BBa_J31003
     misc_feature    64..897
                     /label=BBa_S03709
     misc_feature    910..913
                     /label=conserved
     RBS             906..917
                     /label=BBa_B0034
     CDS             924..2114
                     /label=tetA(C) forward
     primer_bind     924..947
                     /label=Fwd primer J31007
     primer_bind     2095..2114
                     /label=Rev primer J31007
     misc_feature    2112..2114
                     /label=stop
     CDS             924..2114
                     /label=BBa_J31007
     misc_feature    906..2114
                     /label=BBa_S03710
     misc_feature    2127..2130
                     /label=conserved
     RBS             2123..2134
                     /label=BBa_B0034
     CDS             2141..2815
                     /label=mrfp1
     misc_feature    2822..2846
                     /label=Help:Barcodes
     CDS             2141..2821
                     /label=BBa_E1010
     misc_feature    2123..2846
                     /label=BBa_I13502
     misc_feature    906..2846
                     /label=BBa_S03720
     misc_feature    64..2846
                     /label=BBa_S03723
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagaaagagg agaaatacta gatgagccat attcaacggg aaacgtcttg ctccaggccg
      121 cgattaaatt ccaacatgga tgctgattta tatgggtata aatgggctcg cgataatgtc
      181 gggcaatcag gtgcgacaat ctatcgattg tatgggaagc ccgatgcgcc agagttgttt
      241 ctgaaacatg gcaaaggtag cgttgccaat gatgttacag atgagatggt cagactaaac
      301 tggctgacgg aatttatgcc tcttccgacc atcaagcatt ttatccgtac tcctgatgat
      361 gcatggttac tcaccactgc gatccccggg aaaacagcat tccaggtatt agaagaatat
      421 cctgattcag gtgaaaatat tgttgatgcg ctggcagtgt tcctgcgccg gttgcattcg
      481 attcctgttt gtaattgtcc ttttaacagc gatcgcgtat ttcgtctcgc tcaggcgcaa
      541 tcacgaatga ataacggttt ggttgatgcg agtgattttg atgacgagcg taatggctgg
      601 cctgttgaac aagtctggaa agaaatgcat aagcttttgc cattctcacc ggattcagtc
      661 gtcactcatg gtgatttctc acttgataac cttatttttg acgaggggaa attaataggt
      721 tgtattgatg ttggacgagt cggaatcgca gaccgatacc aggatcttgc catcctatgg
      781 aactgcctcg gtgagttttc tccttcatta cagaaacggc tttttcaaaa atatggtatt
      841 gataatcctg atatgaataa attgcagttt catttgatgc tcgatgagtt tttctaatac
      901 tagagaaaga ggagaaatac tagatgaaat ctaacaatgc gctcatcgtc atcctcggca
      961 ccgtcaccct ggatgctgta ggcataggct tggttatgcc ggtactgccg ggcctcttgc
     1021 gggatatcgt ccattccgac agcatcgcca gtcactatgg cgtgctgcta gcgctatatg
     1081 cgttgatgca atttctatgc gcacccgttc tcggagcact gtccgaccgc tttggccgcc
     1141 gcccagtcct gctcgcttcg ctacttggag ccactatcga ctacgcgatc atggcgacca
     1201 cacccgtcct gtggatcctc tacgccggac gcatcgtggc cggcatcacc ggcgccacag
     1261 gtgcggttgc tggcgcctat atcgccgaca tcaccgatgg ggaagatcgg gctcgccact
     1321 tcgggctcat gagcgcttgt ttcggcgtgg gtatggtggc aggccccgtg gccgggggac
     1381 tgttgggcgc catctccttg catgcaccat tccttgcggc ggcggtgctc aacggcctca
     1441 acctactact gggctgcttc ctaatgcagg agtcgcataa gggagagcgt cgaccgatgc
     1501 ccttgagagc cttcaaccca gtcagctcct tccggtgggc gcggggcatg actatcgtcg
     1561 ccgcacttat gactgtcttc tttatcatgc aactcgtagg acaggtgccg gcagcgctct
     1621 gggtcatttt cggcgaggac cgctttcgct ggagcgcgac gatgatcggc ctgtcgcttg
     1681 cggtattcgg aatcttgcac gccctcgctc aagccttcgt cactggcccc gccaccaaac
     1741 gtttcggcga gaagcaggcc attatcgccg gcatggcggc cgacgcgctg ggctacgtct
     1801 tgctggcgtt cgcgacgcga ggctggatgg ccttccccat tatgattctt ctcgcttccg
     1861 gcggcatcgg gatgcccgcg ttgcaggcca tgctgtccag gcaggtagat gacgaccatc
     1921 agggacagct tcaaggatcg ctcgcggctc ttaccagcct aacttcgatc attggaccgc
     1981 tgatcgtcac ggcgatttat gccgcctcgg cgagcacatg gaacgggttg gcatggattg
     2041 taggcgccgc cctatacctt gtctgcctcc ccgcgttgcg tcgcggtgca tggagccggg
     2101 ccacctcgac ctaatactag agaaagagga gaaatactag atggcttcct ccgaagacgt
     2161 tatcaaagag ttcatgcgtt tcaaagttcg tatggaaggt tccgttaacg gtcacgagtt
     2221 cgaaatcgaa ggtgaaggtg aaggtcgtcc gtacgaaggt acccagaccg ctaaactgaa
     2281 agttaccaaa ggtggtccgc tgccgttcgc ttgggacatc ctgtccccgc agttccagta
     2341 cggttccaaa gcttacgtta aacacccggc tgacatcccg gactacctga aactgtcctt
     2401 cccggaaggt ttcaaatggg aacgtgttat gaacttcgaa gacggtggtg ttgttaccgt
     2461 tacccaggac tcctccctgc aagacggtga gttcatctac aaagttaaac tgcgtggtac
     2521 caacttcccg tccgacggtc cggttatgca gaaaaaaacc atgggttggg aagcttccac
     2581 cgaacgtatg tacccggaag acggtgctct gaaaggtgaa atcaaaatgc gtctgaaact
     2641 gaaagacggt ggtcactacg acgctgaagt taaaaccacc tacatggcta aaaaaccggt
     2701 tcagctgccg ggtgcttaca aaaccgacat caaactggac atcacctccc acaacgaaga
     2761 ctacaccatc gttgaacagt acgaacgtgc tgaaggtcgt cactccaccg gtgcttaata
     2821 acgctgatag tgctagtgta gatcgc
//