LOCUS       BBa_I715008             2899 bp    DNA     linear       17-May-2024
DEFINITION  OmpC promoter:RBS:Kan:RBS:Tet:RBS:RFP
ACCESSION   BBa_I715008
VERSION     BBa_I715008.1  
FEATURES             Location/Qualifiers
     misc_feature    13..30
                     /label=C1 OmpR
     misc_feature    34..51
                     /label=C2 OmpR
     misc_feature    54..71
                     /label=C3 OmpR
     promoter        75..80
                     /label=-35
     promoter        98..103
                     /label=-10
     promoter        1..108
                     /label=BBa_R0082
     misc_feature    121..124
                     /label=conserved
     RBS             117..128
                     /label=BBa_B0034
     CDS             135..950
                     /label=KanR Gene
     CDS             135..950
                     /label=BBa_J31003
     misc_feature    117..950
                     /label=BBa_S03709
     misc_feature    963..966
                     /label=conserved
     RBS             959..970
                     /label=BBa_B0034
     CDS             977..2167
                     /label=tetA(C) forward
     primer_bind     977..1000
                     /label=Fwd primer J31007
     primer_bind     2148..2167
                     /label=Rev primer J31007
     misc_feature    2165..2167
                     /label=stop
     CDS             977..2167
                     /label=BBa_J31007
     misc_feature    959..2167
                     /label=BBa_S03710
     misc_feature    2180..2183
                     /label=conserved
     RBS             2176..2187
                     /label=BBa_B0034
     CDS             2194..2868
                     /label=mrfp1
     misc_feature    2875..2899
                     /label=Help:Barcodes
     CDS             2194..2874
                     /label=BBa_E1010
     misc_feature    2176..2899
                     /label=BBa_I13502
     misc_feature    959..2899
                     /label=BBa_S03720
     misc_feature    117..2899
                     /label=BBa_S03723
ORIGIN
        1 tcccttgcat ttacattttg aaacatctat agcgataaat gaaacatctt aaaagtttta
       61 gtatcatatt cgtgttggat tattctgcat ttttggggag aatggactta ctagagaaag
      121 aggagaaata ctagatgagc catattcaac gggaaacgtc ttgctccagg ccgcgattaa
      181 attccaacat ggatgctgat ttatatgggt ataaatgggc tcgcgataat gtcgggcaat
      241 caggtgcgac aatctatcga ttgtatggga agcccgatgc gccagagttg tttctgaaac
      301 atggcaaagg tagcgttgcc aatgatgtta cagatgagat ggtcagacta aactggctga
      361 cggaatttat gcctcttccg accatcaagc attttatccg tactcctgat gatgcatggt
      421 tactcaccac tgcgatcccc gggaaaacag cattccaggt attagaagaa tatcctgatt
      481 caggtgaaaa tattgttgat gcgctggcag tgttcctgcg ccggttgcat tcgattcctg
      541 tttgtaattg tccttttaac agcgatcgcg tatttcgtct cgctcaggcg caatcacgaa
      601 tgaataacgg tttggttgat gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg
      661 aacaagtctg gaaagaaatg cataagcttt tgccattctc accggattca gtcgtcactc
      721 atggtgattt ctcacttgat aaccttattt ttgacgaggg gaaattaata ggttgtattg
      781 atgttggacg agtcggaatc gcagaccgat accaggatct tgccatccta tggaactgcc
      841 tcggtgagtt ttctccttca ttacagaaac ggctttttca aaaatatggt attgataatc
      901 ctgatatgaa taaattgcag tttcatttga tgctcgatga gtttttctaa tactagagaa
      961 agaggagaaa tactagatga aatctaacaa tgcgctcatc gtcatcctcg gcaccgtcac
     1021 cctggatgct gtaggcatag gcttggttat gccggtactg ccgggcctct tgcgggatat
     1081 cgtccattcc gacagcatcg ccagtcacta tggcgtgctg ctagcgctat atgcgttgat
     1141 gcaatttcta tgcgcacccg ttctcggagc actgtccgac cgctttggcc gccgcccagt
     1201 cctgctcgct tcgctacttg gagccactat cgactacgcg atcatggcga ccacacccgt
     1261 cctgtggatc ctctacgccg gacgcatcgt ggccggcatc accggcgcca caggtgcggt
     1321 tgctggcgcc tatatcgccg acatcaccga tggggaagat cgggctcgcc acttcgggct
     1381 catgagcgct tgtttcggcg tgggtatggt ggcaggcccc gtggccgggg gactgttggg
     1441 cgccatctcc ttgcatgcac cattccttgc ggcggcggtg ctcaacggcc tcaacctact
     1501 actgggctgc ttcctaatgc aggagtcgca taagggagag cgtcgaccga tgcccttgag
     1561 agccttcaac ccagtcagct ccttccggtg ggcgcggggc atgactatcg tcgccgcact
     1621 tatgactgtc ttctttatca tgcaactcgt aggacaggtg ccggcagcgc tctgggtcat
     1681 tttcggcgag gaccgctttc gctggagcgc gacgatgatc ggcctgtcgc ttgcggtatt
     1741 cggaatcttg cacgccctcg ctcaagcctt cgtcactggc cccgccacca aacgtttcgg
     1801 cgagaagcag gccattatcg ccggcatggc ggccgacgcg ctgggctacg tcttgctggc
     1861 gttcgcgacg cgaggctgga tggccttccc cattatgatt cttctcgctt ccggcggcat
     1921 cgggatgccc gcgttgcagg ccatgctgtc caggcaggta gatgacgacc atcagggaca
     1981 gcttcaagga tcgctcgcgg ctcttaccag cctaacttcg atcattggac cgctgatcgt
     2041 cacggcgatt tatgccgcct cggcgagcac atggaacggg ttggcatgga ttgtaggcgc
     2101 cgccctatac cttgtctgcc tccccgcgtt gcgtcgcggt gcatggagcc gggccacctc
     2161 gacctaatac tagagaaaga ggagaaatac tagatggctt cctccgaaga cgttatcaaa
     2221 gagttcatgc gtttcaaagt tcgtatggaa ggttccgtta acggtcacga gttcgaaatc
     2281 gaaggtgaag gtgaaggtcg tccgtacgaa ggtacccaga ccgctaaact gaaagttacc
     2341 aaaggtggtc cgctgccgtt cgcttgggac atcctgtccc cgcagttcca gtacggttcc
     2401 aaagcttacg ttaaacaccc ggctgacatc ccggactacc tgaaactgtc cttcccggaa
     2461 ggtttcaaat gggaacgtgt tatgaacttc gaagacggtg gtgttgttac cgttacccag
     2521 gactcctccc tgcaagacgg tgagttcatc tacaaagtta aactgcgtgg taccaacttc
     2581 ccgtccgacg gtccggttat gcagaaaaaa accatgggtt gggaagcttc caccgaacgt
     2641 atgtacccgg aagacggtgc tctgaaaggt gaaatcaaaa tgcgtctgaa actgaaagac
     2701 ggtggtcact acgacgctga agttaaaacc acctacatgg ctaaaaaacc ggttcagctg
     2761 ccgggtgctt acaaaaccga catcaaactg gacatcacct cccacaacga agactacacc
     2821 atcgttgaac agtacgaacg tgctgaaggt cgtcactcca ccggtgctta ataacgctga
     2881 tagtgctagt gtagatcgc
//