LOCUS       BBa_I715017             1045 bp    DNA     linear       21-May-2024
DEFINITION  pLac:RBS.1:Kan
ACCESSION   BBa_I715017
VERSION     BBa_I715017.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     RBS             209..223
                     /label=RBS-1\Strong
     RBS             216..220
                     /label=RBS
     misc_feature    209..223
                     /label=BBa_B0030
     RBS             209..223
                     /label=BBa_B0030
     CDS             230..1045
                     /label=KanR Gene
     CDS             230..1045
                     /label=BBa_J31003
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagat taaagaggag aaatactaga tgagccatat
      241 tcaacgggaa acgtcttgct ccaggccgcg attaaattcc aacatggatg ctgatttata
      301 tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct atcgattgta
      361 tgggaagccc gatgcgccag agttgtttct gaaacatggc aaaggtagcg ttgccaatga
      421 tgttacagat gagatggtca gactaaactg gctgacggaa tttatgcctc ttccgaccat
      481 caagcatttt atccgtactc ctgatgatgc atggttactc accactgcga tccccgggaa
      541 aacagcattc caggtattag aagaatatcc tgattcaggt gaaaatattg ttgatgcgct
      601 ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt ttaacagcga
      661 tcgcgtattt cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg ttgatgcgag
      721 tgattttgat gacgagcgta atggctggcc tgttgaacaa gtctggaaag aaatgcataa
      781 gcttttgcca ttctcaccgg attcagtcgt cactcatggt gatttctcac ttgataacct
      841 tatttttgac gaggggaaat taataggttg tattgatgtt ggacgagtcg gaatcgcaga
      901 ccgataccag gatcttgcca tcctatggaa ctgcctcggt gagttttctc cttcattaca
      961 gaaacggctt tttcaaaaat atggtattga taatcctgat atgaataaat tgcagtttca
     1021 tttgatgctc gatgagtttt tctaa
//