LOCUS       BBa_I715034              950 bp    DNA     linear       18-May-2024
DEFINITION  pLAC-RBS-RFP1-HixC-RFP2
ACCESSION   BBa_I715034
VERSION     BBa_I715034.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    227..229
                     /label=start
     CDS             227..688
                     /label=Coding sequence
     CDS             227..688
                     /label=BBa_I715022
     misc_feature    697..722
                     /label=BBa_J44000
     misc_feature    731..731
                     /label=extra base (avoid frameshift)
     CDS             732..944
                     /label=Continue coding sequence
     misc_feature    945..949
                     /label=(two in tandem)
     CDS             731..950
                     /label=BBa_I715023
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgg cttcctccga
      241 agacgttatc aaagagttca tgcgtttcaa agttcgtatg gaaggttccg ttaacggtca
      301 cgagttcgaa atcgaaggtg aaggtgaagg tcgtccgtac gaaggtaccc agaccgctaa
      361 actgaaagtt accaaaggtg gtccgctgcc gttcgcttgg gacatcctgt ccccgcagtt
      421 ccagtacggt tccaaagctt acgttaaaca cccggctgac atcccggact acctgaaact
      481 gtccttcccg gaaggtttca aatgggaacg tgttatgaac ttcgaagacg gtggtgttgt
      541 taccgttacc caggactcct ccctgcaaga cggtgagttc atctacaaag ttaaactgcg
      601 tggtaccaac ttcccgtccg acggtccggt tatgcagaaa aaaaccatgg gttgggaagc
      661 ttccaccgaa cgtatgtacc cggaagacta ctagagttat caaaaaccat ggtttttgat
      721 aatactagag tggtgctctg aaaggtgaaa tcaaaatgcg tctgaaactg aaagacggtg
      781 gtcactacga cgctgaagtt aaaaccacct acatggctaa aaaaccggtt cagctgccgg
      841 gtgcttacaa aaccgacatc aaactggaca tcacctccca caacgaagac tacaccatcg
      901 ttgaacagta cgaacgtgct gaaggtcgtc actccaccgg tgcttaataa
//