LOCUS       BBa_I715062             1250 bp    DNA     linear       01-Jun-2024
DEFINITION  PLAC-Trp(hix)-RBS-GFP-TT
ACCESSION   BBa_I715062
VERSION     BBa_I715062.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    228..230
                     /label=start
     RBS             213..221
                     /label=predicted RBS
     CDS             228..272
                     /label=leader peptide
     misc_feature    255..260
                     /label=tandem trp codons
     misc_feature    270..272
                     /label=stop
     misc_feature    360..367
                     /label=Poly T
     misc_feature    299..324
                     /label=hixC
     promoter        209..367
                     /label=BBa_I715053
     misc_feature    380..383
                     /label=conserved
     RBS             376..387
                     /label=BBa_B0034
     CDS             394..1113
                     /label=GFP protein
     CDS             394..1113
                     /label=BBa_E0040
     stem_loop       1133..1176
                     /label=stem_loop
     misc_feature    1122..1201
                     /label=BBa_B0010
     terminator      1122..1201
                     /label=BBa_B0010
     stem_loop       1217..1236
                     /label=T7 TE
     misc_feature    1243..1243
                     /label=stop
     polyA_site      1237..1250
                     /label=polya
     misc_feature    1210..1250
                     /label=BBa_B0012
     terminator      1210..1250
                     /label=BBa_B0012
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagcg taaaaagggt atcgacaatg aaagcaattt
      241 tcgtactgaa aggttggtgg cgcacttcct gaaacgggca gtgtattcac catgcgtatt
      301 atcaaaaacc atggtttttg ataaagcaat cagataccca gcccgcctaa tgagcgggct
      361 ttttttttac tagagaaaga ggagaaatac tagatgcgta aaggagaaga acttttcact
      421 ggagttgtcc caattcttgt tgaattagat ggtgatgtta atgggcacaa attttctgtc
      481 agtggagagg gtgaaggtga tgcaacatac ggaaaactta cccttaaatt tatttgcact
      541 actggaaaac tacctgttcc atggccaaca cttgtcacta ctttcggtta tggtgttcaa
      601 tgctttgcga gatacccaga tcatatgaaa cagcatgact ttttcaagag tgccatgccc
      661 gaaggttatg tacaggaaag aactatattt ttcaaagatg acgggaacta caagacacgt
      721 gctgaagtca agtttgaagg tgataccctt gttaatagaa tcgagttaaa aggtattgat
      781 tttaaagaag atggaaacat tcttggacac aaattggaat acaactataa ctcacacaat
      841 gtatacatca tggcagacaa acaaaagaat ggaatcaaag ttaacttcaa aattagacac
      901 aacattgaag atggaagcgt tcaactagca gaccattatc aacaaaatac tccaattggc
      961 gatggccctg tccttttacc agacaaccat tacctgtcca cacaatctgc cctttcgaaa
     1021 gatcccaacg aaaagagaga ccacatggtc cttcttgagt ttgtaacagc tgctgggatt
     1081 acacatggca tggatgaact atacaaataa taatactaga gccaggcatc aaataaaacg
     1141 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct
     1201 ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata
//