LOCUS       BBa_I715064             1180 bp    DNA     linear       17-May-2024
DEFINITION  PBAD-Trp(hix)-RBS-GFP-TT
ACCESSION   BBa_I715064
VERSION     BBa_I715064.1  
FEATURES             Location/Qualifiers
     promoter        1..130
                     /label=BBa_I13453
     misc_feature    158..160
                     /label=start
     RBS             143..151
                     /label=predicted RBS
     CDS             158..202
                     /label=leader peptide
     misc_feature    185..190
                     /label=tandem trp codons
     misc_feature    200..202
                     /label=stop
     misc_feature    290..297
                     /label=Poly T
     misc_feature    229..254
                     /label=hixC
     promoter        139..297
                     /label=BBa_I715053
     misc_feature    310..313
                     /label=conserved
     RBS             306..317
                     /label=BBa_B0034
     CDS             324..1043
                     /label=GFP protein
     CDS             324..1043
                     /label=BBa_E0040
     stem_loop       1063..1106
                     /label=stem_loop
     misc_feature    1052..1131
                     /label=BBa_B0010
     terminator      1052..1131
                     /label=BBa_B0010
     stem_loop       1147..1166
                     /label=T7 TE
     misc_feature    1173..1173
                     /label=stop
     polyA_site      1167..1180
                     /label=polya
     misc_feature    1140..1180
                     /label=BBa_B0012
     terminator      1140..1180
                     /label=BBa_B0012
ORIGIN
        1 acattgatta tttgcacggc gtcacacttt gctatgccat agcattttta tccataagat
       61 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca taccgttttt
      121 ttgggctagc tactagagcg taaaaagggt atcgacaatg aaagcaattt tcgtactgaa
      181 aggttggtgg cgcacttcct gaaacgggca gtgtattcac catgcgtatt atcaaaaacc
      241 atggtttttg ataaagcaat cagataccca gcccgcctaa tgagcgggct ttttttttac
      301 tagagaaaga ggagaaatac tagatgcgta aaggagaaga acttttcact ggagttgtcc
      361 caattcttgt tgaattagat ggtgatgtta atgggcacaa attttctgtc agtggagagg
      421 gtgaaggtga tgcaacatac ggaaaactta cccttaaatt tatttgcact actggaaaac
      481 tacctgttcc atggccaaca cttgtcacta ctttcggtta tggtgttcaa tgctttgcga
      541 gatacccaga tcatatgaaa cagcatgact ttttcaagag tgccatgccc gaaggttatg
      601 tacaggaaag aactatattt ttcaaagatg acgggaacta caagacacgt gctgaagtca
      661 agtttgaagg tgataccctt gttaatagaa tcgagttaaa aggtattgat tttaaagaag
      721 atggaaacat tcttggacac aaattggaat acaactataa ctcacacaat gtatacatca
      781 tggcagacaa acaaaagaat ggaatcaaag ttaacttcaa aattagacac aacattgaag
      841 atggaagcgt tcaactagca gaccattatc aacaaaatac tccaattggc gatggccctg
      901 tccttttacc agacaaccat tacctgtcca cacaatctgc cctttcgaaa gatcccaacg
      961 aaaagagaga ccacatggtc cttcttgagt ttgtaacagc tgctgggatt acacatggca
     1021 tggatgaact atacaaataa taatactaga gccaggcatc aaataaaacg aaaggctcag
     1081 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt
     1141 cacactggct caccttcggg tgggcctttc tgcgtttata
//