LOCUS       BBa_I715069             1065 bp    DNA     linear       01-Jun-2024
DEFINITION  Test part to see if RE and pLac +hixc can be expressed with GFP
ACCESSION   BBa_I715069
VERSION     BBa_I715069.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    209..234
                     /label=BBa_J44000
     misc_feature    247..250
                     /label=conserved
     RBS             243..254
                     /label=BBa_B0034
     CDS             261..980
                     /label=GFP protein
     CDS             261..980
                     /label=BBa_E0040
     misc_feature    1039..1044
                     /label=Former SpeI site
     misc_feature    1039..1039
                     /label=Mutation of SpeI site
     misc_feature    989..1065
                     /label=RE Sequence
     misc_feature    1017..1017
                     /label=Originally a C
     misc_feature    1065..1065
                     /label=Insertion right before biobrick ends
     misc_feature    994..1009
                     /label=Proximal Fis Binding Site
     misc_feature    1043..1057
                     /label=Distal Fis Binding Site
     misc_feature    989..1065
                     /label=BBa_J3101
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagtt atcaaaaacc atggtttttg ataatactag
      241 agaaagagga gaaatactag atgcgtaaag gagaagaact tttcactgga gttgtcccaa
      301 ttcttgttga attagatggt gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg
      361 aaggtgatgc aacatacgga aaacttaccc ttaaatttat ttgcactact ggaaaactac
      421 ctgttccatg gccaacactt gtcactactt tcggttatgg tgttcaatgc tttgcgagat
      481 acccagatca tatgaaacag catgactttt tcaagagtgc catgcccgaa ggttatgtac
      541 aggaaagaac tatatttttc aaagatgacg ggaactacaa gacacgtgct gaagtcaagt
      601 ttgaaggtga tacccttgtt aatagaatcg agttaaaagg tattgatttt aaagaagatg
      661 gaaacattct tggacacaaa ttggaataca actataactc acacaatgta tacatcatgg
      721 cagacaaaca aaagaatgga atcaaagtta acttcaaaat tagacacaac attgaagatg
      781 gaagcgttca actagcagac cattatcaac aaaatactcc aattggcgat ggccctgtcc
      841 ttttaccaga caaccattac ctgtccacac aatctgccct ttcgaaagat cccaacgaaa
      901 agagagacca catggtcctt cttgagtttg taacagctgc tgggattaca catggcatgg
      961 atgaactata caaataataa tactagagtt cgggtgtcaa caattgacca aaatattgat
     1021 ttacagcgta atgcgctttc tagtgcaaat tgtgaccgca ttttg
//