LOCUS       BBa_I718009              982 bp    DNA     linear       09-May-2024
DEFINITION  pTet-lox71-rbs-gfp-ter
ACCESSION   BBa_I718009
VERSION     BBa_I718009.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    63..96
                     /label=BBa_J37028
     RBS             105..119
                     /label=RBS-1\Strong
     RBS             112..116
                     /label=RBS
     misc_feature    105..119
                     /label=BBa_B0030
     RBS             105..119
                     /label=BBa_B0030
     CDS             126..845
                     /label=GFP protein
     CDS             126..845
                     /label=BBa_E0040
     stem_loop       865..908
                     /label=stem_loop
     misc_feature    854..933
                     /label=BBa_B0010
     terminator      854..933
                     /label=BBa_B0010
     stem_loop       949..968
                     /label=T7 TE
     misc_feature    975..975
                     /label=stop
     polyA_site      969..982
                     /label=polya
     misc_feature    942..982
                     /label=BBa_B0012
     terminator      942..982
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agtaccgttc gtatacgata cattatacga agttattact agagattaaa gaggagaaat
      121 actagatgcg taaaggagaa gaacttttca ctggagttgt cccaattctt gttgaattag
      181 atggtgatgt taatgggcac aaattttctg tcagtggaga gggtgaaggt gatgcaacat
      241 acggaaaact tacccttaaa tttatttgca ctactggaaa actacctgtt ccatggccaa
      301 cacttgtcac tactttcggt tatggtgttc aatgctttgc gagataccca gatcatatga
      361 aacagcatga ctttttcaag agtgccatgc ccgaaggtta tgtacaggaa agaactatat
      421 ttttcaaaga tgacgggaac tacaagacac gtgctgaagt caagtttgaa ggtgataccc
      481 ttgttaatag aatcgagtta aaaggtattg attttaaaga agatggaaac attcttggac
      541 acaaattgga atacaactat aactcacaca atgtatacat catggcagac aaacaaaaga
      601 atggaatcaa agttaacttc aaaattagac acaacattga agatggaagc gttcaactag
      661 cagaccatta tcaacaaaat actccaattg gcgatggccc tgtcctttta ccagacaacc
      721 attacctgtc cacacaatct gccctttcga aagatcccaa cgaaaagaga gaccacatgg
      781 tccttcttga gtttgtaaca gctgctggga ttacacatgg catggatgaa ctatacaaat
      841 aataatacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt
      901 cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg
      961 ggtgggcctt tctgcgttta ta
//