BBa_I718015
1
BBa_I718015
ter-lox66-rbs-dapA (B. Subtilis)
2007-10-25T11:00:00Z
2015-08-31T04:07:52Z
none
Released HQ 2013
downstream construct of the synthetic multicellular bacteria
false
false
_141_
0
1481
9
In stock
false
none
false
David Bikard
component1956322
1
BBa_B0010
component1956330
1
BBa_B0030
component1956324
1
BBa_B0012
component1956328
1
BBa_I718016
component1956335
1
BBa_I718005
annotation1956328
1
BBa_I718016
range1956328
1
138
171
annotation1956324
1
BBa_B0012
range1956324
1
89
129
annotation1956335
1
BBa_I718005
range1956335
1
201
1076
annotation1956330
1
BBa_B0030
range1956330
1
180
194
annotation1956322
1
BBa_B0010
range1956322
1
1
80
BBa_I718005
1
BBa_I718005
DapA Bacillus Subtilis
2007-07-22T11:00:00Z
2015-08-31T04:07:52Z
Bacillus subtilis subsp. subtilis str. 168 (strain: 168)
DapA gene of Bacillus Subtilis. This gene is not sensitive to lysine allosteric-feedback contrary to E.Coli DapA.
false
false
_141_
0
1481
9
Not in stock
false
none
false
David Bikard
annotation1939205
1
stop
range1939205
1
871
876
annotation1939206
1
DapA B. Subtilis
range1939206
1
1
876
annotation1939204
1
start
range1939204
1
1
3
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation7018
1
BBa_B0010
range7018
1
1
80
annotation4184
1
stem_loop
range4184
1
12
55
BBa_B0030
1
BBa_B0030
RBS.1 (strong) -- modified from R. Weiss
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_44_46_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation7025
1
BBa_B0030
range7025
1
1
15
annotation1701
1
RBS-1\Strong
range1701
1
1
15
annotation1702
1
RBS
range1702
1
8
12
BBa_I718016
1
lox66
lox66
2007-10-25T11:00:00Z
2015-08-31T04:07:52Z
This part was generated in the form of a forward & a reverse primer. After annealing these primers EcoRI & PstI compatible cohesive ends at the 5' & 3' ends of the dsDNA were generated.
Next, the dsDNA was subcloned in a pSB1A2 open plasmid (digested with EcoRI & PstI)
You can follow the construction process by following the links available in the Paris iGEM 2007 wiki:
http://parts.mit.edu/igem07/index.php/Paris
"freezer" section
plasmids table. A links sends you to the corresponding notebook date when the ligation reaction was performed
lox66 is a site specific recombination cassette. It belongs to the loxP family frequently used in genetics, particularily in mouse genetics.
lox site recombination is catalysed by a Site specific recombinase, Cre.
lox sequences are composed of an 8 bp Core sequence surrounded by two Arms.
The particularity of lox66 is that it has an altered sequence at the end of it's left arm compared to loxP. This sequence variation reduces affinity of the Cre recombinase for the arm.
As a consequence, after a recombination between a lox66 and a lox71 (altered right arm sequence), one of the two resulting generated lox sites has very low recombination potential as it inherited both mutated arms. Use of lox66 & lox71 sites is potentially interresting when the recombination reaction must be "irreversible".
false
false
_141_
0
1568
9
In stock
false
No modidification was made on the lox66 sequence
true
Eimad Shotar
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1686
1
T7 TE
range1686
1
8
27
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1690
1
polya
range1690
1
28
41
annotation1687
1
stop
range1687
1
34
34
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_I718015_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagataacttggtatagcatacattatacgaacggtatactagagattaaagaggagaaatactagatgaatttcggaaatgtgtctacagcgatgattacaccctttgacaataaagggaacgtagactttcaaaaactgtctacactgattgattacttgttgaaaaacggaacggattctttagtagtagcgggaacaactggagaatctccgaccctttcaactgaagaaaaaattgcgctttttgaatatacggtaaaagaagtaaacggacgggtgcccgttatcgctggtactgggagcaacaacacgaaagattccattaagctgacaaaaaaagctgaggaagcgggcgtggacgctgttatgcttgttaccccgtattacaataaaccttctcaagaaggaatgtaccagcattttaaagcaattgcggcagagacatctcttccggttatgctctataatgttcctggccgtacggttgcttctcttgctcctgaaacgacgatccgtttggcggcagacattccgaatgtggttgcgattaaagaagcgagcggagacctcgaagcgattacaaaaattattgctgaaacacctgaagacttctatgtctattcaggggatgatgctctaacgcttccaattctttcagttggaggtagaggagttgtgtcagtggcgagccatattgcaggcactgatatgcagcaaatgatcaaaaattatacgaatgggcaaacagctaatgcggcactgattcatcagaaactgctgccgattatgaaggaactgtttaaagcgcctaatcctgctccagtcaaaacagcgcttcagctgagaggtcttgatgtcggttctgtgcgtctgcctctagtcccattaactgaggatgaacgtctgagcttaagcagcacgatcagcgaactgtaataa
BBa_B0030_sequence
1
attaaagaggagaaa
BBa_I718005_sequence
1
atgaatttcggaaatgtgtctacagcgatgattacaccctttgacaataaagggaacgtagactttcaaaaactgtctacactgattgattacttgttgaaaaacggaacggattctttagtagtagcgggaacaactggagaatctccgaccctttcaactgaagaaaaaattgcgctttttgaatatacggtaaaagaagtaaacggacgggtgcccgttatcgctggtactgggagcaacaacacgaaagattccattaagctgacaaaaaaagctgaggaagcgggcgtggacgctgttatgcttgttaccccgtattacaataaaccttctcaagaaggaatgtaccagcattttaaagcaattgcggcagagacatctcttccggttatgctctataatgttcctggccgtacggttgcttctcttgctcctgaaacgacgatccgtttggcggcagacattccgaatgtggttgcgattaaagaagcgagcggagacctcgaagcgattacaaaaattattgctgaaacacctgaagacttctatgtctattcaggggatgatgctctaacgcttccaattctttcagttggaggtagaggagttgtgtcagtggcgagccatattgcaggcactgatatgcagcaaatgatcaaaaattatacgaatgggcaaacagctaatgcggcactgattcatcagaaactgctgccgattatgaaggaactgtttaaagcgcctaatcctgctccagtcaaaacagcgcttcagctgagaggtcttgatgtcggttctgtgcgtctgcctctagtcccattaactgaggatgaacgtctgagcttaagcagcacgatcagcgaactgtaataa
BBa_I718016_sequence
1
ataacttggtatagcatacattatacgaacggta
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z