LOCUS       BBa_I721006             2748 bp    DNA     linear       03-May-2024
DEFINITION  pLac>ERBS>TetR>ERBS>AraC>ERBS>EYFP+AAV>Terminator
ACCESSION   BBa_I721006
VERSION     BBa_I721006.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     CDS             230..846
                     /label=tetR
     misc_feature    847..880
                     /label=SsrA
     misc_feature    887..911
                     /label=Help:Barcodes
     CDS             227..911
                     /label=BBa_C0040
     misc_feature    209..911
                     /label=BBa_S03518
     misc_feature    924..927
                     /label=conserved
     RBS             920..931
                     /label=BBa_B0034
     CDS             938..1813
                     /label=araC
     misc_feature    1814..1846
                     /label=LVA
     misc_feature    1853..1877
                     /label=Help:Barcodes
     CDS             938..1877
                     /label=BBa_C0080
     misc_feature    920..1877
                     /label=BBa_S03519
     misc_feature    2640..2645
                     /label=stop
     misc_feature    2601..2639
                     /label=AAV
     misc_feature    1884..2645
                     /label=BBa_E0034
     stem_loop       2661..2680
                     /label=T7 TE
     misc_feature    2687..2687
                     /label=stop
     polyA_site      2681..2694
                     /label=polya
     misc_feature    2654..2694
                     /label=BBa_B0012
     terminator      2654..2694
                     /label=BBa_B0012
     stem_loop       2715..2737
                     /label=stem_loop
     misc_feature    2703..2748
                     /label=BBa_B0011
     terminator      2703..2748
                     /label=BBa_B0011
     terminator      2654..2748
                     /label=BBa_B0014
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgt ccagattaga
      241 taaaagtaaa gtgattaaca gcgcattaga gctgcttaat gaggtcggaa tcgaaggttt
      301 aacaacccgt aaactcgccc agaagctagg tgtagagcag cctacattgt attggcatgt
      361 aaaaaataag cgggctttgc tcgacgcctt agccattgag atgttagata ggcaccatac
      421 tcacttttgc cctttagaag gggaaagctg gcaagatttt ttacgtaata acgctaaaag
      481 ttttagatgt gctttactaa gtcatcgcga tggagcaaaa gtacatttag gtacacggcc
      541 tacagaaaaa cagtatgaaa ctctcgaaaa tcaattagcc tttttatgcc aacaaggttt
      601 ttcactagag aatgcattat atgcactcag cgctgtgggg cattttactt taggttgcgt
      661 attggaagat caagagcatc aagtcgctaa agaagaaagg gaaacaccta ctactgatag
      721 tatgccgcca ttattacgac aagctatcga attatttgat caccaaggtg cagagccagc
      781 cttcttattc ggccttgaat tgatcatatg cggattagaa aaacaactta aatgtgaaag
      841 tgggtccgct gcaaacgacg aaaactacgc tttagtagct taataacact gatagtgcta
      901 gtgtagatca ctactagaga aagaggagaa atactagatg gctgaagcgc aaaatgatcc
      961 cctgctgccg ggatactcgt ttaacgccca tctggtggcg ggtttaacgc cgattgaggc
     1021 caatggttat ctcgattttt ttatcgaccg accgctggga atgaaaggtt atattctcaa
     1081 tctcaccatt cgcggtcagg gggtggtgaa aaatcaggga cgagaatttg tctgccgacc
     1141 gggtgatatt ttgctgttcc cgccaggaga gattcatcac tacggtcgtc atccggaggc
     1201 tcgcgaatgg tatcaccagt gggtttactt tcgtccgcgc gcctactggc atgaatggct
     1261 taactggccg tcaatatttg ccaatacggg tttctttcgc ccggatgaag cgcaccagcc
     1321 gcatttcagc gacctgtttg ggcaaatcat taacgccggg caaggggaag ggcgctattc
     1381 ggagctgctg gcgataaatc tgcttgagca attgttactg cggcgcatgg aagcgattaa
     1441 cgagtcgctc catccaccga tggataatcg ggtacgcgag gcttgtcagt acatcagcga
     1501 tcacctggca gacagcaatt ttgatatcgc cagcgtcgca cagcatgttt gcctgtcgcc
     1561 gtcgcgtctg tcacatcttt tccgccagca gttagggatt agcgtcttaa gctggcgcga
     1621 ggaccaacgc atcagccagg cgaagctgct tttgagcact acccggatgc ctatcgccac
     1681 cgtcggtcgc aatgttggtt ttgacgatca actctatttc tcgcgagtat ttaaaaaatg
     1741 caccggggcc agcccgagcg agttccgtgc cggttgtgaa gaaaaagtga atgatgtagc
     1801 cgtcaagttg tcagctgcaa acgacgaaaa ctacgcttta gtagcttaat aacactgata
     1861 gtgctagtgt agatcactac tagatggtga gcaagggcga ggagctgttc accggggtgg
     1921 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg
     1981 agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca
     2041 agctgcccgt gccctggccc accctcgtga ccaccttcgg ctacggcctg caatgcttcg
     2101 cccgctaccc cgaccacatg aagctgcacg acttcttcaa gtccgccatg cccgaaggct
     2161 acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg
     2221 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg
     2281 aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata
     2341 tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg
     2401 aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc
     2461 ccgtgctgct gcccgacaac cactacctga gctaccagtc cgccctgagc aaagacccca
     2521 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg
     2581 gcatggacga gctgtacaag aggcctgctg caaacgacga aaactacgct gctgctgttt
     2641 aataatacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tatatactag
     2701 agagagaata taaaaagcca gattattaat ccggcttttt tattattt
//