BBa_I723030 1 BBa_I723030 DntR plus double terminator 2007-10-25T11:00:00Z 2015-08-31T04:07:54Z DntR cloned from a historical plasmid based on pQF52 carrying genomic sequences from Burkholderia cepacia. RBS taken from iGEM source plate. Transcriptional regulator DntR's cds plus a double terminator. DntR binds salicylate and activates transcription from promoter pDntA. false false _126_ 0 2140 9 It's complicated false false Scott Ramsay component1956505 1 BBa_B0010 component1956507 1 BBa_B0012 component1956504 1 BBa_I723010 annotation1956505 1 BBa_B0010 range1956505 1 915 994 annotation1956504 1 BBa_I723010 range1956504 1 1 906 annotation1956507 1 BBa_B0012 range1956507 1 1003 1043 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 BBa_I723010 1 DntR DntR coding region 2007-09-05T11:00:00Z 2015-08-31T04:07:53Z Cloned from [IL DntR in pQF52]. The protein encoded by DntR recognises environmental [salicylate] and undergoes a conformational change allowing it to, and positively regulate, the promoter for the gene DntA. DntR, therefore, should be used in combination with pDntA to acheive a switch that responds to [salicylate]. false false _126_ 0 2140 9 Not in stock false This transcriptional regulator is encoded in the opposite direction to that of its target promoter. false Scott Ramsay annotation1954937 1 DntR range1954937 1 1 906 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_I723030_sequence 1 aatacgaagtctcttttcgagctgcttgttgactgcatcggtgtacaacgggcctagggccaacatgaaccgtacggttttgtccaactaccgctacagtccgtcgaaccggcccacgcccctacagcagtctggttttccgtgaagcgtcgcttgccgttttgcgacgccgtgccagcgctactccagccacgacacgtcttaccccggctagcgttactttacgccgtggtggtcggcgtacgcggaaaactacggacgcgcaagctcgtccggtagctggagtggcacaggccacaactcacgctggtgcggctgtacgaggtcaagtcacttgacaaagtccgagtaccccctaaaccgcgaacctaccaggaacgccttgtacgtatgcatcgccaccgctttctccgcggcgaccttcttaggccagacatcgagaccgtcttctgggttccgctctagttggcgtggcctgaggtataggaggaagtctaacggtcgtaagcccgcgtcgcacgactagacctacactcctcgagcaacgcgttcgcgaaggtagtcacccccgtacttcatgtagagcggctacagccagtaacggttcaacttccacgcgcacgaccgtttacccagctttctcagtgcccagcagtcacggcagacgtcgcacaactcgcgtatctagtgcccgaggcgttctacgtcacgtatgccacagccgaggtacggaaaactccacgcgttcttgtttagcagcaagtcgcgacatgcgtcggcaaattcacttaatgactgccgtccgacgcagtcggggtcaaaaagcggccggcagctatgcgaggccagctcgtcatcgaccaacttctggtggtcgtctaagttcagctacagcgcgtctaggtatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_I723010_sequence 1 aatacgaagtctcttttcgagctgcttgttgactgcatcggtgtacaacgggcctagggccaacatgaaccgtacggttttgtccaactaccgctacagtccgtcgaaccggcccacgcccctacagcagtctggttttccgtgaagcgtcgcttgccgttttgcgacgccgtgccagcgctactccagccacgacacgtcttaccccggctagcgttactttacgccgtggtggtcggcgtacgcggaaaactacggacgcgcaagctcgtccggtagctggagtggcacaggccacaactcacgctggtgcggctgtacgaggtcaagtcacttgacaaagtccgagtaccccctaaaccgcgaacctaccaggaacgccttgtacgtatgcatcgccaccgctttctccgcggcgaccttcttaggccagacatcgagaccgtcttctgggttccgctctagttggcgtggcctgaggtataggaggaagtctaacggtcgtaagcccgcgtcgcacgactagacctacactcctcgagcaacgcgttcgcgaaggtagtcacccccgtacttcatgtagagcggctacagccagtaacggttcaacttccacgcgcacgaccgtttacccagctttctcagtgcccagcagtcacggcagacgtcgcacaactcgcgtatctagtgcccgaggcgttctacgtcacgtatgccacagccgaggtacggaaaactccacgcgttcttgtttagcagcaagtcgcgacatgcgtcggcaaattcacttaatgactgccgtccgacgcagtcggggtcaaaaagcggccggcagctatgcgaggccagctcgtcatcgaccaacttctggtggtcgtctaagttcagctacagcgcgtctaggta BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z