BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1690
1
polya
range1690
1
28
41
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1686
1
T7 TE
range1686
1
8
27
annotation1687
1
stop
range1687
1
34
34
BBa_B0021
1
BBa_B0021
LuxICDABEG (+/-), reversed
2003-10-13T11:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Bidirectional transcriptional terminator cloned in the reverse direction of B0011. 22 bp stem-loop
false
true
_1_
0
24
7
In stock
false
Cloned with primer-dimers into pSB1A2
true
Caitlin Conboy
annotation7024
1
BBa_B0021
range7024
1
1
46
BBa_J45002
1
BAMT
SAM:benzoic acid carboxyl methyltransferase; converts benzoic acid to methyl benzoate (floral odor)
2006-06-06T11:00:00Z
2015-08-31T04:08:49Z
Genbank accession number(s): AF198492
This part is from A. majus.
BAMT catalyzes the transfer of a methyl group from SAM (S-adenosyl methyltransferase) to benzoic acid, creating methyl benzoate. Methyl benzoate produces a "pleasant" smell.
false
false
_84_
0
977
84
It's complicated
true
none as of yet
false
Andr?? Green II
annotation1880475
1
putative start codon 1
range1880475
1
1
3
annotation1880476
1
putative start codon 2
range1880476
1
10
12
annotation1880495
1
double TAA stop codon
range1880495
1
1093
1098
annotation1880494
1
BAMT
range1880494
1
1
1095
annotation1880506
1
A -> G to eliminate SpeI site
range1880506
1
702
702
BBa_B0011
1
BBa_B0011
LuxICDABEG (+/-)
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from luxICDABEG operon terminator of Vibrio fischeri <genbank>AF170104</genbank>.
Released HQ 2013
Bidirectional transcriptional terminator consisting of a 22 bp stem-loop.</p>
false
false
_1_
0
24
7
In stock
false
<P> <P>In the naturally-occuring sequence there is a mismatch in the stem of the stem loop. This can be corrected via an A->G mutation (at position 40 -- sequence coordinate/not MFOLD coordinate). The above sequence does not reflect this mutation (but the MFOLD image does). This terminator's location cannot be found using some inverted repeat detectors like PALINDROME because it is too short and contains a mismatch. This one was found with the help of Tom Knight. It lies between two coding regions that point towards eachother.<P>
true
Reshma Shetty
annotation1683
1
stem_loop
range1683
1
13
35
annotation7019
1
BBa_B0011
range7019
1
1
46
BBa_B0032
1
BBa_B0032
RBS.3 (medium) -- derivative of BBa_0030
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_41_44_48_46_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). <P>
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation7027
1
BBa_B0032
range7027
1
1
13
annotation1709
1
RBS-3\Weak
range1709
1
1
13
annotation1710
1
RBS
range1710
1
7
10
BBa_I723125
1
BBa_I723125
Test reporter again
2007-07-30T11:00:00Z
2015-08-31T04:07:54Z
From my head again
Long desc
true
false
_126_
0
2129
9
Discontinued
false
design considerations
false
Maciej Trybilo
component1940411
1
BBa_J45002
component1940412
1
BBa_B0012
component1940404
1
BBa_B0032
component1940418
1
BBa_B0021
component1940416
1
BBa_B0011
annotation1940416
1
BBa_B0011
range1940416
1
1175
1220
annotation1940404
1
BBa_B0032
range1940404
1
1
13
annotation1940418
1
BBa_B0021
range1940418
1
1229
1274
annotation1940411
1
BBa_J45002
range1940411
1
20
1117
annotation1940412
1
BBa_B0012
range1940412
1
1126
1166
BBa_I723125_sequence
1
tcacacaggaaagtactagatgaaagtgatgaagaaacttttgtgtatgaatattgcaggagatggtgaaactagctacgccaacaattctggccttcaaaaagttatgatgtcaaaatcattgcatgttttagacgaaacccttaaagatattatcggtgatcatgttggcttcccaaaatgcttcaagatgatggatatgggttgttcatcagggcctaacgcccttttggtcatgtccggcattataaatacaattgaggatttgtacacagagaagaatattaatgaattacctgaatttgaggtttttctgaacgatcttccagacaacgacttcaacaacctcttcaaattgttatcacatgagaatggaaactgctttgtatatggtttgcctggatctttctacgggagactattgccaaaaaagagcctacactttgcttattcttcctacagtattcactggctctctcaggttcctgaagggctggaggataataacagacaaaacatttacatggcaacggaaagtcctccggaagtgtacaaagcatacgcaaagcaatacgaaagagacttctccacatttctaaagttgcgaggcgaggaaattgtaccaggtggacgcatggtcttgacatttaacggcagaagtgttgaagatccctcgagcaaagatgacttagcaattttcacattgcttgcaaaaacactggttgatatggtggctgaggggcttgtcaagatggacgatttgtactcgtttaacattcctatttactcaccatgtacgcgcgaagtagaggcagcaattctgagtgaagggtcttttacgttggacaggctagaggtctttcgtgtttgttgggatgcaagtgactacacagatgacgatgatcagcaagacccatcaatctttggcaaacaaaggagtggaaaatttgtggcagattgtgtacgggctattacggaaccaatgctggctagccattttgggagcactattatggatcttctatttggaaagtatgcaaagaaaatagtggagcatctatctgtggagaactcgtcatatttcagcatagtagtttctctaagtaggagataataatactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattttactagagaaataataaaaaagccggattaataatctggctttttatattctct
BBa_B0021_sequence
1
aaataataaaaaagccggattaataatctggctttttatattctct
BBa_J45002_sequence
1
atgaaagtgatgaagaaacttttgtgtatgaatattgcaggagatggtgaaactagctacgccaacaattctggccttcaaaaagttatgatgtcaaaatcattgcatgttttagacgaaacccttaaagatattatcggtgatcatgttggcttcccaaaatgcttcaagatgatggatatgggttgttcatcagggcctaacgcccttttggtcatgtccggcattataaatacaattgaggatttgtacacagagaagaatattaatgaattacctgaatttgaggtttttctgaacgatcttccagacaacgacttcaacaacctcttcaaattgttatcacatgagaatggaaactgctttgtatatggtttgcctggatctttctacgggagactattgccaaaaaagagcctacactttgcttattcttcctacagtattcactggctctctcaggttcctgaagggctggaggataataacagacaaaacatttacatggcaacggaaagtcctccggaagtgtacaaagcatacgcaaagcaatacgaaagagacttctccacatttctaaagttgcgaggcgaggaaattgtaccaggtggacgcatggtcttgacatttaacggcagaagtgttgaagatccctcgagcaaagatgacttagcaattttcacattgcttgcaaaaacactggttgatatggtggctgaggggcttgtcaagatggacgatttgtactcgtttaacattcctatttactcaccatgtacgcgcgaagtagaggcagcaattctgagtgaagggtcttttacgttggacaggctagaggtctttcgtgtttgttgggatgcaagtgactacacagatgacgatgatcagcaagacccatcaatctttggcaaacaaaggagtggaaaatttgtggcagattgtgtacgggctattacggaaccaatgctggctagccattttgggagcactattatggatcttctatttggaaagtatgcaaagaaaatagtggagcatctatctgtggagaactcgtcatatttcagcatagtagtttctctaagtaggagataataa
BBa_B0032_sequence
1
tcacacaggaaag
BBa_B0011_sequence
1
agagaatataaaaagccagattattaatccggcttttttattattt
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z