BBa_I725099 1 BBa_I725099 Partial operon for butanoate pathway 2007-10-23T11:00:00Z 2015-08-31T04:07:54Z Genes were synthesized from E. coli-optimized Clostridium acetobutylicum sequences from KEGG. The butanoate pathway in Clostridium acetobutylicum from acetyl-CoA to butanol requires five (or six) enzymes. In order, these are acetyl-CoA acetyltransferase (thiolase in E. coli), B-hydroxy butyryl coA dehydrogenase, Enoyl-coA hydratase (from E. coli), Butyryl coA Dehydrogenase, Butyraldehyde dehydrogenase, and Butanol dehydrogenase. It is unclear whether the E. coli thiolase will be sufficient for butanol production in E. coli or whether overexpression will be required. Subsequently, it is not included in this version of the operon. false false _106_ 0 1839 9 It's complicated false We were unable to complete addition of the fifth gene to the operon (Butanol dehydrogenase). It needs to be cloned from I725025 to complete this. false Wayne Materi component1954030 1 BBa_B0034 component1954028 1 BBa_I72512 component1954025 1 BBa_I725011 component1954033 1 BBa_B0034 component1954027 1 BBa_B0034 component1954024 1 BBa_B0034 component1954031 1 BBa_I725013 component1954034 1 BBa_I725014 annotation1954028 1 BBa_I72512 range1954028 1 915 1715 annotation1954025 1 BBa_I725011 range1954025 1 19 888 annotation1954024 1 BBa_B0034 range1954024 1 1 12 annotation1954034 1 BBa_I725014 range1954034 1 2923 5520 annotation1954027 1 BBa_B0034 range1954027 1 897 908 annotation1954030 1 BBa_B0034 range1954030 1 1724 1735 annotation1954033 1 BBa_B0034 range1954033 1 2905 2916 annotation1954031 1 BBa_I725013 range1954031 1 1742 2896 BBa_I725013 1 BBa_I725013 Butyryl CoA dehyrogenase 2007-06-17T11:00:00Z 2015-08-31T04:07:54Z C. acetobutylicum genom. CAC_2712 Butyryl coa dehyrogenase from C. acetobutylicum. The sequence has been selected because it is part of an operon that has successfully been cloned into E. coli. It catalyzes the third step in butanol biosythesis. It has a His-tag added to its C terminus. false false _106_ 0 1824 9 It's complicated false The sequence has been optimized for use in E. Coli and has had its restriction sites removed. It has had a c-terminal His tag added and its stop codon has been changed to TAATAA. false Erin Dul BBa_I725011 1 BBa_I725011 B-hydroxy butyryl coA dehydrogenase 2007-06-17T11:00:00Z 2015-08-31T04:07:54Z Genomic sequence of Chlostridium acetobutylicum. CAC_2708. B-hydroxybutyryl coa dehydrogenase from C. acetobutylicum. Catalyzes the first step in the conversion of aceto-acetylcoa into butanol. It is from an operon containing this gene as well as two other genes in the butanol production pathway. It has been successfully cloned into e. coli before and activity has been recovered. The sequence has C-terminal His-tag. false false _106_ 0 1824 9 It's complicated false The sequence has been optimized for E. Coli and has had its restriction sites removed. A His tag has been added to the C terminus. The stop codon has been changed to TAATAA. true Erin Dul BBa_I72512 1 BBa_I72512 Enoyl-coa hydratase 2007-06-17T11:00:00Z 2015-08-31T04:07:54Z C. acetobutylicum genome. CAC_2712 Enoly-coa hydratase from C. acetobutylicum. The sequence has been selected because it is part of an operon that has successfully been cloned into E. coli. It catalyzes the second step in butanol biosythesis. It has a His-tag added to its C terminus. false false _106_ 0 1824 9 It's complicated false The sequence has had its codons optimized for E. coli and has had its restriction sites removed. The sequence has had its stop codon changed to TAATAA. It has had a His tag added to its c terminus. true Erin Dul BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_I725014 1 BBa_I725014 Butyraldehyde dehydrogenase 2007-06-17T11:00:00Z 2015-08-31T04:07:54Z pSOL plamid of C. acetobutylicum. CAP_0035 This is the butyraldehyde dehydrogenase from C. acetobutylicum. It catalyzes the fourth step in the reaction of butanol biosythesis. Its is from the pSOL plasmid of C. acetobutylicum. It also has some alcohol dehydrogenase activity. false false _106_ 0 1824 9 It's complicated false The sequence has been optimized for use in E. Coli and has had its restriction sites removed. It has had a c-terminal His tag added and its stop codon has been changed to TAATAA. false Erin Dul BBa_I725014_sequence 1 atgaaagttaccaaccagaaagaactgaaacagaaactgaacgaactgcgtgaagctcagaaaaaattcgctacctacacccaggaacaggttgacaaaatcttcaaacagtgcgctatcgctgctgctaaagaacgtatcaacctggctaaactggctgttgaagaaaccggtatcggtctggttgaagacaaaatcatcaaaaaccacttcgctgctgaatacatctacaacaaatacaaaaacgaaaaaacctgcggtatcatcgaccacgacgactccctgggtatcaccaaagttgctgaaccgatcggtatcgttgctgctatcgttccgaccaccaacccgacctccaccgctatcttcaaatccctgatctccctgaaaacccgtaacgctatcttcttctccccgcacccgcgtgctaaaaaatccaccatcgctgctgctaaactgatcctggacgctgctgttaaagctggtgctccgaaaaacatcatcggttggatcgacgaaccgtccatcgaactgtcccaggacctgatgtccgaagctgacatcatcctggctaccggtggtccgtccatggttaaagctgcttactcctccggtaaaccggctatcggtgttggtgctggtaacaccccggctatcatcgacgaatccgctgacatcgacatggctgtttcctccatcatcctgtccaaaacctacgacaacggtgttatctgcgcttccgaacagtccatcctggttatgaactccatctacgaaaaagttaaagaagagttcgttaaacgtggttcctacatcctgaaccagaacgaaatcgctaaaatcaaagaaaccatgttcaaaaacggtgctatcaacgctgacatcgttggtaaatccgcttacatcatcgctaaaatggctggtatcgaagttccgcagaccaccaaaatcctgatcggtgaagttcagtccgttgaaaaatccgaactgttctcccacgaaaaactgtccccggttctggctatgtacaaagttaaagacttcgacgaagctctgaaaaaagctcagcgtctgatcgaactgggtggttccggtcacacctcctccctgtacatcgactcccagaacaacaaagacaaagttaaagagttcggtctggctatgaaaacctcccgtaccttcatcaacatgccgtcctcccagggtgcttccggtgacctgtacaacttcgctatcgctccgtccttcaccctgggttgcggtacctggggtggtaactccgtttcccagaacgttgaaccgaaacacctgctgaacatcaaatccgttgctgaacgtcgtgaaaacatgctgtggttcaaagttccgcagaaaatctacttcaaatacggttgcctgcgtttcgctctgaaagaactgaaagacatgaacaaaaaacgtgctttcatcgttaccgacaaagacctgttcaaactgggttacgttaacaaaatcaccaaagttctggacgaaatcgacatcaaatactccatcttcaccgacatcaaatccgacccgaccatcgactccgttaaaaaaggtgctaaagaaatgctgaacttcgaaccggacaccatcatctccatcggtggtggttccccgatggacgctgctaaagttatgcacctgctgtacgaatacccggaagctgaaatcgaaaacctggctatcaacttcatggacatccgtaaacgtatctgcaacttcccgaaactgggtaccaaagctatctccgttgctatcccgaccaccgctggtaccggttccgaagctaccccgttcgctgttatcaccaacgacgaaaccggtatgaaatacccgctgacctcctacgaactgaccccgaacatggctatcatcgacaccgaactgatgctgaacatgccgcgtaaactgaccgctgctaccggtatcgacgctctggttcacgctatcgaagcttacgtttccgttatggctaccgactacaccgacgaactggctctgcgtgctatcaaaatgatcttcaaatacctgccgcgtgcttacaaaaacggtaccaacgacatcgaagctcgtgaaaaaatggctcacgcttccaacatcgctggtatggctttcgctaacgctttcctgggtgtttgccactccatggctcacaaactgggtgctatgcaccacgttccgcacggtatcgcttgcgctgttctgatcgaagaagttatcaaatacaacgctaccgactgcccgaccaaacagaccgctttcccgcagtacaaatccccgaacgctaaacgtaaatacgctgaaatcgctgaatacctgaacctgaaaggtacctccgacaccgaaaaagttaccgctctgatcgaagctatctccaaactgaaaatcgacctgtccatcccgcagaacatctccgctgctggtatcaacaaaaaagacttctacaacaccctggacaaaatgtccgaactggctttcgacgaccagtgcaccaccgctaacccgcgttacccgctgatctccgaactgaaagacatctacatcaaatccttccatcaccatcaccatcactaataa BBa_I725013_sequence 1 atggacttcaacctgacccgtgaacaggaactggttcgtcagatggttcgtgagttcgctgaaaacgaagttaaaccgatcgctgctgaaatcgacgaaaccgaacgtttcccgatggaaaacgttaaaaaaatgggtcagtacggtatgatgggtatcccgttctccaaagaatacggtggtgctggtggtgacgttctgtcctacatcatcgctgttgaagaactgtccaaagtttgcggtaccaccggtgttatcctgtccgctcacacctccctgtgcgcttccctgatcaacgaacacggtaccgaagaacagaaacagaaatacctggttccgctggctaaaggtgaaaaaatcggtgcttacggtctgaccgaaccgaacgctggtaccgactccggtgctcagcagaccgttgctgttctggaaggtgaccactacgttatcaacggttccaaaatcttcatcaccaacggtggtgttgctgacaccttcgttatcttcgctatgaccgaccgtaccaaaggtaccaaaggtatctccgctttcatcatcgaaaaaggtttcaaaggtttctccatcggtaaagttgaacagaaactgggtatccgtgcttcctccaccaccgaactggttttcgaagacatgatcgttccggttgaaaacatgatcggtaaagaaggtaaaggtttcccgatcgctatgaaaaccctggacggtggtcgtatcggtatcgctgctcaggctctgggtatcgctgaaggtgctttcaacgaagctcgtgcttacatgaaagaacgtaaacagttcggtcgttccctggacaaattccagggtctggcttggatgatggctgacatggacgttgctatcgaatccgctcgttacctggtttacaaagctgcttacctgaaacaggctggtctgccgtacaccgttgacgctgctcgtgctaaactgcacgctgctaacgttgctatggacgttaccaccaaagctgttcagctgttcggtggttacggttacaccaaagactacccggttgaacgtatgatgcgtgacgctaaaatcaccgaaatctacgaaggtacctccgaagttcagaaactggttatctccggtaaaatcttccgtcatcaccatcaccatcac BBa_B0034_sequence 1 aaagaggagaaa BBa_I725011_sequence 1 atgaaaaaagtttgcgttatcggtgctggtaccatgggttccggtatcgctcaggctttcgctgctaaaggtttcgaagttgttctgcgtgacatcaaagacgaatttgttgaccgtggtctggacttcatcaacaaaaacctgtccaaactggttaaaaaaggtaaaatcgaagaagctaccaaagttgaaatcctgacccgtatctccggtaccgttgacctgaacatggctgctgactgcgacctggttatcgaagctgctgttgaacgtatggacatcaaaaaacagatcttcgctgacctggacaacatctgcaaaccggaaaccatcctggcttccaacacctcctccctgtccatcaccgaagttgcttccgctaccaaacgtccggacaaagttatcggtatgcacttcttcaacccggctccggttatgaaactggttgaagttatccgtggtatcgctacctcccaggaaaccttcgacgctgttaaagaaacctccatcgctatcggtaaagacccggttgaagttgctgaagctccgggtttcgttgttaaccgtatcctgatcccgatgatcaacgaagctgttggtatcctggctgaaggtatcgcttccgttgaagacatcgacaaagctatgaaactgggtgctaaccacccgatgggtccgctggaactgggtgacttcatcggtctggacatctgcctggctatcatggacgttctgtactccgaaaccggtgactccaaataccgtccgcacaccctgctgaaaaaatacgttcgtgctggttggctgggtcgtaaatccggtaaaggtttctacgactactccaaacatcaccatcaccatcactaataa BBa_I725099_sequence 1 aaagaggagaaatactagatgaaaaaagtttgcgttatcggtgctggtaccatgggttccggtatcgctcaggctttcgctgctaaaggtttcgaagttgttctgcgtgacatcaaagacgaatttgttgaccgtggtctggacttcatcaacaaaaacctgtccaaactggttaaaaaaggtaaaatcgaagaagctaccaaagttgaaatcctgacccgtatctccggtaccgttgacctgaacatggctgctgactgcgacctggttatcgaagctgctgttgaacgtatggacatcaaaaaacagatcttcgctgacctggacaacatctgcaaaccggaaaccatcctggcttccaacacctcctccctgtccatcaccgaagttgcttccgctaccaaacgtccggacaaagttatcggtatgcacttcttcaacccggctccggttatgaaactggttgaagttatccgtggtatcgctacctcccaggaaaccttcgacgctgttaaagaaacctccatcgctatcggtaaagacccggttgaagttgctgaagctccgggtttcgttgttaaccgtatcctgatcccgatgatcaacgaagctgttggtatcctggctgaaggtatcgcttccgttgaagacatcgacaaagctatgaaactgggtgctaaccacccgatgggtccgctggaactgggtgacttcatcggtctggacatctgcctggctatcatggacgttctgtactccgaaaccggtgactccaaataccgtccgcacaccctgctgaaaaaatacgttcgtgctggttggctgggtcgtaaatccggtaaaggtttctacgactactccaaacatcaccatcaccatcactaataatactagagaaagaggagaaatactagatggaactgaacaacgttatcctggaaaaagaaggtaaagttgctgttgttaccatcaaccgtccgaaagctctgaacgctctgaactccgacaccctgaaagaaatggactacgttatcggtgaaatcgaaaacgactccgaagttctggctgttatcctgaccggtgctggtgaaaaatccttcgttgctggtgctgacatctccgaaatgaaagaaatgaacaccatcgaaggtcgtaaattcggtatcctgggtaacaaagttttccgtcgtctggaactgctggaaaaaccggttatcgctgctgttaacggtttcgctctgggtggtggttgcgaaatcgctatgtcctgcgacatccgtatcgcttcctccaacgctcgtttcggtcagccggaagttggtctgggtatcaccccgggtttcggtggtacccagcgtctgtcccgtctggttggtatgggtatggctaaacagctgatcttcaccgctcagaacatcaaagctgacgaagctctgcgtatcggtctggttaacaaagttgttgaaccgtccgaactgatgaacaccgctaaagaaatcgctaacaaaatcgtttccaacgctccggttgctgttaaactgtccaaacaggctatcaaccgtggtatgcagtgcgacatcgacaccgctctggctttcgaatccgaagctttcggtgaatgcttctccaccgaagaccagaaagacgctatgaccgctttcatcgaaaaacgtaaaatcgaaggtttcaaaaaccgtcatcaccatcaccatcactactagagaaagaggagaaatactagatggacttcaacctgacccgtgaacaggaactggttcgtcagatggttcgtgagttcgctgaaaacgaagttaaaccgatcgctgctgaaatcgacgaaaccgaacgtttcccgatggaaaacgttaaaaaaatgggtcagtacggtatgatgggtatcccgttctccaaagaatacggtggtgctggtggtgacgttctgtcctacatcatcgctgttgaagaactgtccaaagtttgcggtaccaccggtgttatcctgtccgctcacacctccctgtgcgcttccctgatcaacgaacacggtaccgaagaacagaaacagaaatacctggttccgctggctaaaggtgaaaaaatcggtgcttacggtctgaccgaaccgaacgctggtaccgactccggtgctcagcagaccgttgctgttctggaaggtgaccactacgttatcaacggttccaaaatcttcatcaccaacggtggtgttgctgacaccttcgttatcttcgctatgaccgaccgtaccaaaggtaccaaaggtatctccgctttcatcatcgaaaaaggtttcaaaggtttctccatcggtaaagttgaacagaaactgggtatccgtgcttcctccaccaccgaactggttttcgaagacatgatcgttccggttgaaaacatgatcggtaaagaaggtaaaggtttcccgatcgctatgaaaaccctggacggtggtcgtatcggtatcgctgctcaggctctgggtatcgctgaaggtgctttcaacgaagctcgtgcttacatgaaagaacgtaaacagttcggtcgttccctggacaaattccagggtctggcttggatgatggctgacatggacgttgctatcgaatccgctcgttacctggtttacaaagctgcttacctgaaacaggctggtctgccgtacaccgttgacgctgctcgtgctaaactgcacgctgctaacgttgctatggacgttaccaccaaagctgttcagctgttcggtggttacggttacaccaaagactacccggttgaacgtatgatgcgtgacgctaaaatcaccgaaatctacgaaggtacctccgaagttcagaaactggttatctccggtaaaatcttccgtcatcaccatcaccatcactactagagaaagaggagaaatactagatgaaagttaccaaccagaaagaactgaaacagaaactgaacgaactgcgtgaagctcagaaaaaattcgctacctacacccaggaacaggttgacaaaatcttcaaacagtgcgctatcgctgctgctaaagaacgtatcaacctggctaaactggctgttgaagaaaccggtatcggtctggttgaagacaaaatcatcaaaaaccacttcgctgctgaatacatctacaacaaatacaaaaacgaaaaaacctgcggtatcatcgaccacgacgactccctgggtatcaccaaagttgctgaaccgatcggtatcgttgctgctatcgttccgaccaccaacccgacctccaccgctatcttcaaatccctgatctccctgaaaacccgtaacgctatcttcttctccccgcacccgcgtgctaaaaaatccaccatcgctgctgctaaactgatcctggacgctgctgttaaagctggtgctccgaaaaacatcatcggttggatcgacgaaccgtccatcgaactgtcccaggacctgatgtccgaagctgacatcatcctggctaccggtggtccgtccatggttaaagctgcttactcctccggtaaaccggctatcggtgttggtgctggtaacaccccggctatcatcgacgaatccgctgacatcgacatggctgtttcctccatcatcctgtccaaaacctacgacaacggtgttatctgcgcttccgaacagtccatcctggttatgaactccatctacgaaaaagttaaagaagagttcgttaaacgtggttcctacatcctgaaccagaacgaaatcgctaaaatcaaagaaaccatgttcaaaaacggtgctatcaacgctgacatcgttggtaaatccgcttacatcatcgctaaaatggctggtatcgaagttccgcagaccaccaaaatcctgatcggtgaagttcagtccgttgaaaaatccgaactgttctcccacgaaaaactgtccccggttctggctatgtacaaagttaaagacttcgacgaagctctgaaaaaagctcagcgtctgatcgaactgggtggttccggtcacacctcctccctgtacatcgactcccagaacaacaaagacaaagttaaagagttcggtctggctatgaaaacctcccgtaccttcatcaacatgccgtcctcccagggtgcttccggtgacctgtacaacttcgctatcgctccgtccttcaccctgggttgcggtacctggggtggtaactccgtttcccagaacgttgaaccgaaacacctgctgaacatcaaatccgttgctgaacgtcgtgaaaacatgctgtggttcaaagttccgcagaaaatctacttcaaatacggttgcctgcgtttcgctctgaaagaactgaaagacatgaacaaaaaacgtgctttcatcgttaccgacaaagacctgttcaaactgggttacgttaacaaaatcaccaaagttctggacgaaatcgacatcaaatactccatcttcaccgacatcaaatccgacccgaccatcgactccgttaaaaaaggtgctaaagaaatgctgaacttcgaaccggacaccatcatctccatcggtggtggttccccgatggacgctgctaaagttatgcacctgctgtacgaatacccggaagctgaaatcgaaaacctggctatcaacttcatggacatccgtaaacgtatctgcaacttcccgaaactgggtaccaaagctatctccgttgctatcccgaccaccgctggtaccggttccgaagctaccccgttcgctgttatcaccaacgacgaaaccggtatgaaatacccgctgacctcctacgaactgaccccgaacatggctatcatcgacaccgaactgatgctgaacatgccgcgtaaactgaccgctgctaccggtatcgacgctctggttcacgctatcgaagcttacgtttccgttatggctaccgactacaccgacgaactggctctgcgtgctatcaaaatgatcttcaaatacctgccgcgtgcttacaaaaacggtaccaacgacatcgaagctcgtgaaaaaatggctcacgcttccaacatcgctggtatggctttcgctaacgctttcctgggtgtttgccactccatggctcacaaactgggtgctatgcaccacgttccgcacggtatcgcttgcgctgttctgatcgaagaagttatcaaatacaacgctaccgactgcccgaccaaacagaccgctttcccgcagtacaaatccccgaacgctaaacgtaaatacgctgaaatcgctgaatacctgaacctgaaaggtacctccgacaccgaaaaagttaccgctctgatcgaagctatctccaaactgaaaatcgacctgtccatcccgcagaacatctccgctgctggtatcaacaaaaaagacttctacaacaccctggacaaaatgtccgaactggctttcgacgaccagtgcaccaccgctaacccgcgttacccgctgatctccgaactgaaagacatctacatcaaatccttccatcaccatcaccatcactaataa BBa_I72512_sequence 1 atggaactgaacaacgttatcctggaaaaagaaggtaaagttgctgttgttaccatcaaccgtccgaaagctctgaacgctctgaactccgacaccctgaaagaaatggactacgttatcggtgaaatcgaaaacgactccgaagttctggctgttatcctgaccggtgctggtgaaaaatccttcgttgctggtgctgacatctccgaaatgaaagaaatgaacaccatcgaaggtcgtaaattcggtatcctgggtaacaaagttttccgtcgtctggaactgctggaaaaaccggttatcgctgctgttaacggtttcgctctgggtggtggttgcgaaatcgctatgtcctgcgacatccgtatcgcttcctccaacgctcgtttcggtcagccggaagttggtctgggtatcaccccgggtttcggtggtacccagcgtctgtcccgtctggttggtatgggtatggctaaacagctgatcttcaccgctcagaacatcaaagctgacgaagctctgcgtatcggtctggttaacaaagttgttgaaccgtccgaactgatgaacaccgctaaagaaatcgctaacaaaatcgtttccaacgctccggttgctgttaaactgtccaaacaggctatcaaccgtggtatgcagtgcgacatcgacaccgctctggctttcgaatccgaagctttcggtgaatgcttctccaccgaagaccagaaagacgctatgaccgctttcatcgaaaaacgtaaaatcgaaggtttcaaaaaccgtcatcaccatcaccatcac igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z