LOCUS       BBa_I728003             1993 bp    DNA     linear       27-May-2024
DEFINITION  nothing
ACCESSION   BBa_I728003
VERSION     BBa_I728003.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     RBS             63..77
                     /label=RBS-1\Strong
     RBS             70..74
                     /label=RBS
     misc_feature    63..77
                     /label=BBa_B0030
     RBS             63..77
                     /label=BBa_B0030
     misc_feature    84..86
                     /label=baobao!
     CDS             84..1856
                     /label=BBa_I728000
     stem_loop       1876..1919
                     /label=stem_loop
     misc_feature    1865..1944
                     /label=BBa_B0010
     terminator      1865..1944
                     /label=BBa_B0010
     stem_loop       1960..1979
                     /label=T7 TE
     misc_feature    1986..1986
                     /label=stop
     polyA_site      1980..1993
                     /label=polya
     misc_feature    1953..1993
                     /label=BBa_B0012
     terminator      1953..1993
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agattaaaga ggagaaatac tagatgcgct cgctgaagcc gcttgcggcc ttgcccgatc
      121 gcttgctcga tcgcctggtg cactgggcgc gcgtgcgccc cgagcagacc ttcattgccg
      181 cccgcgaggc cggtggcgac tggcgccttg tcagctatgg gcagatgctc gacagcgtgc
      241 gcgccatcgc ccagggcctg cttcgttacg gcctgtcggc cgacaagccg ctggcgctgc
      301 tctcgggcaa tgacatcgaa cacctgcaac tggccctggg cgcgatgtac gccgggattc
      361 cctattgccc ggtgtcgccg gcctattcgc tgctgtccca ggactttgcc aagctgcgcc
      421 atgtctgcga cctgttgcag ccggggctgg tgttcgtcag cgacgccagc gcctaccagc
      481 gggccatcga cgcggtgctg ccgcccgaga cgccgctgat cagcgtgcgt ggccaggtgc
      541 cggggcggcg ccaggcgagc ttcgccagcc tgctggccga gcccggtggc gccgaggccg
      601 atgcggcctt tgccgccacc ggccctgaca gcatcgccaa gtttctcttc acctcgggct
      661 cgaccaagct gcccaaggcg gtgatcacca cccagcgcat gctctgcgcc aaccagcaga
      721 tgctgctgca gacctttccg gtgttcggtg aagagccgcc ggtgctggtg gactggctgc
      781 cgtggaacca caccttcggc ggcagccata acgtcggcat cgtgctctac aacggcggta
      841 ctttttacct ggacgagggc aagcccacgg cccaaggctt cgctgaaacc ctgcgcaacc
      901 tcaaggagat ttcccccacc gcctacctga ccgtgcccaa gggctgggag gaactggtca
      961 acgccctgga gcaggacgcc gagctgcgcg agtgtttttt caagcgcatg agcctgtttt
     1021 tcttcgccgc cgccgggctt tcgcaaagtg tctgggatcg cctggaccgg gtcgccgagc
     1081 agcattgcgg cgaacgcatc cgcatgatgg ccgggctcgg catgaccgag gccgcgccgt
     1141 cttgcacctt caccaccgga ccgctgtcga tggccggtta catcggcctg ccggcgccgg
     1201 gttgcgaagt gcgcctggtg ccggtggatg gcaagctcga agggcgtttt cgcgggccgc
     1261 acatcatgcc cggctactgg cgcgcggcgc agcagaccgc cgaggtgttc gacgcccagg
     1321 gcttctactg ctcgggggat gcgctcaagc tcgccgacgc cgccgacccg caactggggc
     1381 tgatgttcga cgggcgcatc gccgaggact tcaagttgtc gtccggggtg ttcgtcagcg
     1441 tcggaccgct gcgcaatcgc gcggtgctcg aaggcgcgcc ctatgtccag gacctggtgg
     1501 tggccgcccc cgaccgcgag tgcctggggg ccctggtgtt cccgcgcctg tatgagtgcc
     1561 ggcggctggc ggggctgggc gccgaggcca gcgaggccca ggtgctggcc agcgcgccgg
     1621 tgcgccagtg gttcggcgac tggttgcagt gcctgaatcg tgaggccagc ggcaatgcca
     1681 gccgcctgga gtggatcgcg ctgcaggtgg agccggcgtc catcgaccgt ggggagatca
     1741 ccgacaaggg ctcgatcaat cagcgcgcgg tgctgcagtg gcgcgccgag caggtcgaga
     1801 acctgtatcg cggccgcgag ccgtcgatcc tgcgcgccga gcccaagcca taataatact
     1861 agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc
     1921 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct
     1981 ttctgcgttt ata
//