BBa_I728000
1
BBa_I728000
Pseudomonas fluorescens enzyme for vanilla scent synthesis
2007-05-25T11:00:00Z
2015-08-31T04:07:55Z
GenBank Accession number: AJ536325
test
false
true
_138_
0
1536
9
Not in stock
false
Change stop codon to TAATAA.
false
Forrest Liau
annotation1933711
1
baobao!
range1933711
1
1
3
BBa_R0040
1
p(tetR)
TetR repressible promoter
2003-01-31T12:00:00Z
2015-05-08T01:14:14Z
Lutz, R., Bujard, H., <em>Nucleic Acids Research</em> (1997) 25, 1203-1210.
Released HQ 2013
Sequence for pTet inverting regulator driven by the TetR protein.</P>
false
true
_1_
0
24
7
In stock
false
<P> <P>BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.<P>
true
June Rhee, Connie Tao, Ty Thomson, Louis Waldman
annotation1986785
1
-35
range1986785
1
20
25
annotation1986783
1
TetR 1
range1986783
1
1
19
annotation1986784
1
BBa_R0040
range1986784
1
1
54
annotation1986787
1
-10
range1986787
1
43
48
annotation1986786
1
TetR 2
range1986786
1
26
44
BBa_I728003
1
BBa_I728003
nothing
2007-05-25T11:00:00Z
2015-08-31T04:07:55Z
nowhere
useless
false
false
_138_
0
1536
9
Not in stock
false
be considerate!
false
Forrest Liau
component1933822
1
BBa_I728000
component1933814
1
BBa_R0040
component1933819
1
BBa_B0030
component1933823
1
BBa_B0010
component1933825
1
BBa_B0012
annotation1933814
1
BBa_R0040
range1933814
1
1
54
annotation1933819
1
BBa_B0030
range1933819
1
63
77
annotation1933822
1
BBa_I728000
range1933822
1
84
1856
annotation1933825
1
BBa_B0012
range1933825
1
1953
1993
annotation1933823
1
BBa_B0010
range1933823
1
1865
1944
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1686
1
T7 TE
range1686
1
8
27
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1687
1
stop
range1687
1
34
34
annotation1690
1
polya
range1690
1
28
41
BBa_B0030
1
BBa_B0030
RBS.1 (strong) -- modified from R. Weiss
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_44_46_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation7025
1
BBa_B0030
range7025
1
1
15
annotation1702
1
RBS
range1702
1
8
12
annotation1701
1
RBS-1\Strong
range1701
1
1
15
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation7018
1
BBa_B0010
range7018
1
1
80
annotation4184
1
stem_loop
range4184
1
12
55
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_R0040_sequence
1
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
BBa_B0030_sequence
1
attaaagaggagaaa
BBa_I728003_sequence
1
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagattaaagaggagaaatactagatgcgctcgctgaagccgcttgcggccttgcccgatcgcttgctcgatcgcctggtgcactgggcgcgcgtgcgccccgagcagaccttcattgccgcccgcgaggccggtggcgactggcgccttgtcagctatgggcagatgctcgacagcgtgcgcgccatcgcccagggcctgcttcgttacggcctgtcggccgacaagccgctggcgctgctctcgggcaatgacatcgaacacctgcaactggccctgggcgcgatgtacgccgggattccctattgcccggtgtcgccggcctattcgctgctgtcccaggactttgccaagctgcgccatgtctgcgacctgttgcagccggggctggtgttcgtcagcgacgccagcgcctaccagcgggccatcgacgcggtgctgccgcccgagacgccgctgatcagcgtgcgtggccaggtgccggggcggcgccaggcgagcttcgccagcctgctggccgagcccggtggcgccgaggccgatgcggcctttgccgccaccggccctgacagcatcgccaagtttctcttcacctcgggctcgaccaagctgcccaaggcggtgatcaccacccagcgcatgctctgcgccaaccagcagatgctgctgcagacctttccggtgttcggtgaagagccgccggtgctggtggactggctgccgtggaaccacaccttcggcggcagccataacgtcggcatcgtgctctacaacggcggtactttttacctggacgagggcaagcccacggcccaaggcttcgctgaaaccctgcgcaacctcaaggagatttcccccaccgcctacctgaccgtgcccaagggctgggaggaactggtcaacgccctggagcaggacgccgagctgcgcgagtgttttttcaagcgcatgagcctgtttttcttcgccgccgccgggctttcgcaaagtgtctgggatcgcctggaccgggtcgccgagcagcattgcggcgaacgcatccgcatgatggccgggctcggcatgaccgaggccgcgccgtcttgcaccttcaccaccggaccgctgtcgatggccggttacatcggcctgccggcgccgggttgcgaagtgcgcctggtgccggtggatggcaagctcgaagggcgttttcgcgggccgcacatcatgcccggctactggcgcgcggcgcagcagaccgccgaggtgttcgacgcccagggcttctactgctcgggggatgcgctcaagctcgccgacgccgccgacccgcaactggggctgatgttcgacgggcgcatcgccgaggacttcaagttgtcgtccggggtgttcgtcagcgtcggaccgctgcgcaatcgcgcggtgctcgaaggcgcgccctatgtccaggacctggtggtggccgcccccgaccgcgagtgcctgggggccctggtgttcccgcgcctgtatgagtgccggcggctggcggggctgggcgccgaggccagcgaggcccaggtgctggccagcgcgccggtgcgccagtggttcggcgactggttgcagtgcctgaatcgtgaggccagcggcaatgccagccgcctggagtggatcgcgctgcaggtggagccggcgtccatcgaccgtggggagatcaccgacaagggctcgatcaatcagcgcgcggtgctgcagtggcgcgccgagcaggtcgagaacctgtatcgcggccgcgagccgtcgatcctgcgcgccgagcccaagccataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_I728000_sequence
1
atgcgctcgctgaagccgcttgcggccttgcccgatcgcttgctcgatcgcctggtgcactgggcgcgcgtgcgccccgagcagaccttcattgccgcccgcgaggccggtggcgactggcgccttgtcagctatgggcagatgctcgacagcgtgcgcgccatcgcccagggcctgcttcgttacggcctgtcggccgacaagccgctggcgctgctctcgggcaatgacatcgaacacctgcaactggccctgggcgcgatgtacgccgggattccctattgcccggtgtcgccggcctattcgctgctgtcccaggactttgccaagctgcgccatgtctgcgacctgttgcagccggggctggtgttcgtcagcgacgccagcgcctaccagcgggccatcgacgcggtgctgccgcccgagacgccgctgatcagcgtgcgtggccaggtgccggggcggcgccaggcgagcttcgccagcctgctggccgagcccggtggcgccgaggccgatgcggcctttgccgccaccggccctgacagcatcgccaagtttctcttcacctcgggctcgaccaagctgcccaaggcggtgatcaccacccagcgcatgctctgcgccaaccagcagatgctgctgcagacctttccggtgttcggtgaagagccgccggtgctggtggactggctgccgtggaaccacaccttcggcggcagccataacgtcggcatcgtgctctacaacggcggtactttttacctggacgagggcaagcccacggcccaaggcttcgctgaaaccctgcgcaacctcaaggagatttcccccaccgcctacctgaccgtgcccaagggctgggaggaactggtcaacgccctggagcaggacgccgagctgcgcgagtgttttttcaagcgcatgagcctgtttttcttcgccgccgccgggctttcgcaaagtgtctgggatcgcctggaccgggtcgccgagcagcattgcggcgaacgcatccgcatgatggccgggctcggcatgaccgaggccgcgccgtcttgcaccttcaccaccggaccgctgtcgatggccggttacatcggcctgccggcgccgggttgcgaagtgcgcctggtgccggtggatggcaagctcgaagggcgttttcgcgggccgcacatcatgcccggctactggcgcgcggcgcagcagaccgccgaggtgttcgacgcccagggcttctactgctcgggggatgcgctcaagctcgccgacgccgccgacccgcaactggggctgatgttcgacgggcgcatcgccgaggacttcaagttgtcgtccggggtgttcgtcagcgtcggaccgctgcgcaatcgcgcggtgctcgaaggcgcgccctatgtccaggacctggtggtggccgcccccgaccgcgagtgcctgggggccctggtgttcccgcgcctgtatgagtgccggcggctggcggggctgggcgccgaggccagcgaggcccaggtgctggccagcgcgccggtgcgccagtggttcggcgactggttgcagtgcctgaatcgtgaggccagcggcaatgccagccgcctggagtggatcgcgctgcaggtggagccggcgtccatcgaccgtggggagatcaccgacaagggctcgatcaatcagcgcgcggtgctgcagtggcgcgccgagcaggtcgagaacctgtatcgcggccgcgagccgtcgatcctgcgcgccgagcccaagccataataa
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z