BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1690
1
polya
range1690
1
28
41
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1686
1
T7 TE
range1686
1
8
27
annotation1687
1
stop
range1687
1
34
34
BBa_B0011
1
BBa_B0011
LuxICDABEG (+/-)
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from luxICDABEG operon terminator of Vibrio fischeri <genbank>AF170104</genbank>.
Released HQ 2013
Bidirectional transcriptional terminator consisting of a 22 bp stem-loop.</p>
false
false
_1_
0
24
7
In stock
false
<P> <P>In the naturally-occuring sequence there is a mismatch in the stem of the stem loop. This can be corrected via an A->G mutation (at position 40 -- sequence coordinate/not MFOLD coordinate). The above sequence does not reflect this mutation (but the MFOLD image does). This terminator's location cannot be found using some inverted repeat detectors like PALINDROME because it is too short and contains a mismatch. This one was found with the help of Tom Knight. It lies between two coding regions that point towards eachother.<P>
true
Reshma Shetty
annotation1683
1
stem_loop
range1683
1
13
35
annotation7019
1
BBa_B0011
range7019
1
1
46
BBa_I14032
1
P(Lac) IQ
promoter P(Lac) IQ
2004-08-03T11:00:00Z
2015-08-31T04:07:37Z
Plasmid pMAL-p2X
Released HQ 2013
Constitutive Promoter, High Transcription
false
true
_4_
0
171
7
In stock
false
true
Vikram Vijayan, Allen Hsu, Lawrence Fomundam
annotation1028342
1
P(Lac) IQ
range1028342
1
1
37
annotation1028343
1
-35
range1028343
1
3
8
annotation1028344
1
-10
range1028344
1
26
31
BBa_I728500
1
CPX
CPX Terminal Surface Display Protein with Polystyrene-Binding Peptide
2007-07-30T11:00:00Z
2015-08-31T04:07:55Z
Rice JJ, Schohn A, Bessette PH, Boulware KT, and Daugherty PS. Bacterial display using circularly permuted outer membrane protein OmpX yields high affinity peptide ligands. Protein Sci 2006 Apr; 15(4) 825-36. doi:10.1110/ps.051897806 pmid:16600968
This will be filled in later.
false
false
_138_
0
1695
9
It's complicated
false
Max of XXXX amino acid long peptide.
true
Toan Tran-Phu
annotation1940508
1
Double stop codon (TGATAA)
range1940508
1
649
654
annotation1975607
1
Coding sequence
range1975607
1
1
654
annotation1940503
1
Polystyrene Sticky Peptide
range1940503
1
91
129
annotation1940500
1
Start codon
range1940500
1
1
3
annotation1940511
1
SalI Restriction Site
range1940511
1
130
135
annotation1940512
1
Trypsin Cleavage Site
range1940512
1
136
141
annotation1940507
1
A1-S53 of OmpX
range1940507
1
490
648
annotation1940509
1
BglII Cut Site
range1940509
1
85
90
annotation1940502
1
SfiI Restriction Site
range1940502
1
70
84
annotation1940506
1
Linker
range1940506
1
478
489
annotation1940505
1
S54-F148 of OmpX
range1940505
1
193
477
annotation1940504
1
Linker
range1940504
1
175
192
annotation1940510
1
T7 Antibody Tag
range1940510
1
142
174
annotation1940501
1
Native Starting Signal
range1940501
1
1
69
BBa_I728979
1
BBa_I728979
I14032+B0034+BBa_I728000+B0014
2007-10-22T11:00:00Z
2015-08-31T04:07:55Z
Bba_I728978
This is in improvement on part Bba_I728978. F2620 was replaced with a constitutive promoter and a double terminator stop was added after the CPX protein.
false
false
_138_
0
1555
167
It's complicated
false
After obtaining extra unexpected bands on a western blot test of bacteria transformed with Bba_I1728978, we decided to add a double terminator to see if there was leaky transcription into the vector backbone.
false
Bernice Huang
component1975670
1
BBa_B0012
component1975654
1
BBa_B0034
component1975674
1
BBa_B0011
component1975652
1
BBa_I14032
component1975669
1
BBa_I728500
annotation1975669
1
BBa_I728500
range1975669
1
64
717
annotation1975652
1
BBa_I14032
range1975652
1
1
37
annotation1975654
1
BBa_B0034
range1975654
1
46
57
annotation1975670
1
BBa_B0012
range1975670
1
726
766
annotation1975674
1
BBa_B0011
range1975674
1
775
820
BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_I728500_sequence
1
atgaaaaaaattgcgtgcctgagcgcgctggccgcggttctggcctttaccgcgggcaccagcgttgcgggccagtctggccaaagatcttttttcagctttttttttccggcgagcgcgtggggtagcgtcgacaaacgtatggcgagcatgaccggcggtcagcagatgggtggtggccagagcggccagagcggtgattataacaaaaaccagtattatggcattaccgcgggtccggcgtatcgtattaacgattgggcgagcatttatggcgtggtgggcgtgggctatggcaaatttcagaccaccgaatatccgacctataaacacgataccagcgattatggctttagctatggcgcgggtctgcaatttaatccgatggaaaacgtggcgctggattttagctatgaacagagccgtattcgtagcgtggatgtgggcacctggattgcgggcgtgggttatcgttttggcggcagcggtgcgaccagcaccgtgaccggcggttatgcgcagagcgatgcgcagggccagatgaacaaaatgggcggcttcaacctgaaatatcgctatgaagaagataacagcccgctgggcgtgattggcagctttacctataccgaaaaaagccgtaccgcgagctgataa
BBa_B0034_sequence
1
aaagaggagaaa
BBa_I14032_sequence
1
tggtgcaaaacctttcgcggtatggcatgatagcgcc
BBa_I728979_sequence
1
tggtgcaaaacctttcgcggtatggcatgatagcgcctactagagaaagaggagaaatactagatgaaaaaaattgcgtgcctgagcgcgctggccgcggttctggcctttaccgcgggcaccagcgttgcgggccagtctggccaaagatcttttttcagctttttttttccggcgagcgcgtggggtagcgtcgacaaacgtatggcgagcatgaccggcggtcagcagatgggtggtggccagagcggccagagcggtgattataacaaaaaccagtattatggcattaccgcgggtccggcgtatcgtattaacgattgggcgagcatttatggcgtggtgggcgtgggctatggcaaatttcagaccaccgaatatccgacctataaacacgataccagcgattatggctttagctatggcgcgggtctgcaatttaatccgatggaaaacgtggcgctggattttagctatgaacagagccgtattcgtagcgtggatgtgggcacctggattgcgggcgtgggttatcgttttggcggcagcggtgcgaccagcaccgtgaccggcggttatgcgcagagcgatgcgcagggccagatgaacaaaatgggcggcttcaacctgaaatatcgctatgaagaagataacagcccgctgggcgtgattggcagctttacctataccgaaaaaagccgtaccgcgagctgataatactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
BBa_B0011_sequence
1
agagaatataaaaagccagattattaatccggcttttttattattt
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z