LOCUS       BBa_I730010             1807 bp    DNA     linear       28-May-2024
DEFINITION  pOmpR-pLacI-TetR repressor-CFP
ACCESSION   BBa_I730010
VERSION     BBa_I730010.1  
FEATURES             Location/Qualifiers
     misc_feature    13..30
                     /label=C1 OmpR
     misc_feature    34..51
                     /label=C2 OmpR
     misc_feature    54..71
                     /label=C3 OmpR
     promoter        75..80
                     /label=-35
     promoter        98..103
                     /label=-10
     promoter        1..108
                     /label=BBa_R0082
     misc_feature    119..135
                     /label=lac O1
     promoter        136..141
                     /label=-35
     misc_feature    142..158
                     /label=lac O1
     promoter        159..164
                     /label=-10
     misc_feature    117..170
                     /label=BBa_R0011
     promoter        117..170
                     /label=BBa_R0011
     misc_feature    184..187
                     /label=conserved
     RBS             180..191
                     /label=BBa_B0034
     CDS             201..817
                     /label=tetR
     misc_feature    818..851
                     /label=SsrA
     misc_feature    858..882
                     /label=Help:Barcodes
     CDS             198..882
                     /label=BBa_C0040
     misc_feature    895..898
                     /label=conserved
     RBS             891..902
                     /label=BBa_B0034
     CDS             909..1670
                     /label=CFP (LVA)
     misc_feature    1627..1664
                     /label=SsrA
     misc_feature    1665..1670
                     /label=2
     misc_feature    977..977
                     /label=A
     misc_feature    909..1670
                     /label=BBa_E0022
     misc_feature    909..1670
                     /label=BBa_E0022
     stem_loop       1690..1733
                     /label=stem_loop
     misc_feature    1679..1758
                     /label=BBa_B0010
     terminator      1679..1758
                     /label=BBa_B0010
     stem_loop       1774..1793
                     /label=T7 TE
     misc_feature    1800..1800
                     /label=stop
     polyA_site      1794..1807
                     /label=polya
     misc_feature    1767..1807
                     /label=BBa_B0012
     terminator      1767..1807
                     /label=BBa_B0012
     misc_feature    891..1807
                     /label=BBa_E0422
ORIGIN
        1 tcccttgcat ttacattttg aaacatctat agcgataaat gaaacatctt aaaagtttta
       61 gtatcatatt cgtgttggat tattctgcat ttttggggag aatggactta ctagagaatt
      121 gtgagcggat aacaattgac attgtgagcg gataacaaga tactgagcac atactagaga
      181 aagaggagaa atactagatg tccagattag ataaaagtaa agtgattaac agcgcattag
      241 agctgcttaa tgaggtcgga atcgaaggtt taacaacccg taaactcgcc cagaagctag
      301 gtgtagagca gcctacattg tattggcatg taaaaaataa gcgggctttg ctcgacgcct
      361 tagccattga gatgttagat aggcaccata ctcacttttg ccctttagaa ggggaaagct
      421 ggcaagattt tttacgtaat aacgctaaaa gttttagatg tgctttacta agtcatcgcg
      481 atggagcaaa agtacattta ggtacacggc ctacagaaaa acagtatgaa actctcgaaa
      541 atcaattagc ctttttatgc caacaaggtt tttcactaga gaatgcatta tatgcactca
      601 gcgctgtggg gcattttact ttaggttgcg tattggaaga tcaagagcat caagtcgcta
      661 aagaagaaag ggaaacacct actactgata gtatgccgcc attattacga caagctatcg
      721 aattatttga tcaccaaggt gcagagccag ccttcttatt cggccttgaa ttgatcatat
      781 gcggattaga aaaacaactt aaatgtgaaa gtgggtccgc tgcaaacgac gaaaactacg
      841 ctttagtagc ttaataacac tgatagtgct agtgtagatc actactagag aaagaggaga
      901 aatactagat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
      961 agctggacgg cgacgtgaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg
     1021 ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct
     1081 ggcccaccct cgtgaccacc ctgacctggg gcgtgcagtg cttcagccgc taccccgacc
     1141 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca
     1201 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg
     1261 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc
     1321 tggggcacaa gctggagtac aactacatca gccacaacgt ctatatcacc gccgacaagc
     1381 agaagaacgg catcaaggcc aacttcaaga tccgccacaa catcgaggac ggcagcgtgc
     1441 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg
     1501 acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc
     1561 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt
     1621 acaagaggcc tgctgcaaac gacgaaaact acgctttagt agcttaataa tactagagcc
     1681 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt
     1741 ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc
     1801 gtttata
//