LOCUS       BBa_I732724             1225 bp    DNA     linear       01-Jun-2024
DEFINITION  P_NOR_U035O44D001O22 + Double Reporters
ACCESSION   BBa_I732724
VERSION     BBa_I732724.1  
FEATURES             Location/Qualifiers
     promoter        1..82
                     /label=BBa_I732352
     misc_feature    95..98
                     /label=conserved
     RBS             91..102
                     /label=BBa_B0034
     CDS             109..342
                     /label=lacZ
     misc_feature    109..111
                     /label=START
     misc_feature    337..342
                     /label=STOP
     CDS             109..342
                     /label=BBa_I732006
     misc_feature    355..358
                     /label=conserved
     RBS             351..362
                     /label=BBa_B0034
     CDS             369..1088
                     /label=GFP protein
     CDS             369..1088
                     /label=BBa_E0040
     stem_loop       1108..1151
                     /label=stem_loop
     misc_feature    1097..1176
                     /label=BBa_B0010
     terminator      1097..1176
                     /label=BBa_B0010
     stem_loop       1192..1211
                     /label=T7 TE
     misc_feature    1218..1218
                     /label=stop
     polyA_site      1212..1225
                     /label=polya
     misc_feature    1185..1225
                     /label=BBa_B0012
     terminator      1185..1225
                     /label=BBa_B0012
ORIGIN
        1 aattgtgaac gttcacaatt tacactttat gcttccggct cgtataatgt gtggaattgt
       61 aagcgcttac aattggatcc ggtactagag aaagaggaga aatactagat gaccatgatt
      121 acggattcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
      181 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
      241 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgctt tgcctggttt
      301 ccggcaccag aagcggtgcc ggaaagctgg ctggagtaat aatactagag aaagaggaga
      361 aatactagat gcgtaaagga gaagaacttt tcactggagt tgtcccaatt cttgttgaat
      421 tagatggtga tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa
      481 catacggaaa acttaccctt aaatttattt gcactactgg aaaactacct gttccatggc
      541 caacacttgt cactactttc ggttatggtg ttcaatgctt tgcgagatac ccagatcata
      601 tgaaacagca tgactttttc aagagtgcca tgcccgaagg ttatgtacag gaaagaacta
      661 tatttttcaa agatgacggg aactacaaga cacgtgctga agtcaagttt gaaggtgata
      721 cccttgttaa tagaatcgag ttaaaaggta ttgattttaa agaagatgga aacattcttg
      781 gacacaaatt ggaatacaac tataactcac acaatgtata catcatggca gacaaacaaa
      841 agaatggaat caaagttaac ttcaaaatta gacacaacat tgaagatgga agcgttcaac
      901 tagcagacca ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt ttaccagaca
      961 accattacct gtccacacaa tctgcccttt cgaaagatcc caacgaaaag agagaccaca
     1021 tggtccttct tgagtttgta acagctgctg ggattacaca tggcatggat gaactataca
     1081 aataataata ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc
     1141 tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct
     1201 tcgggtgggc ctttctgcgt ttata
//