LOCUS       BBa_I732725             1308 bp    DNA     linear       28-May-2024
DEFINITION  P_U097NUL+D062NUL + Double Reporters
ACCESSION   BBa_I732725
VERSION     BBa_I732725.1  
FEATURES             Location/Qualifiers
     promoter        1..165
                     /label=BBa_I732400
     misc_feature    178..181
                     /label=conserved
     RBS             174..185
                     /label=BBa_B0034
     CDS             192..425
                     /label=lacZ
     misc_feature    192..194
                     /label=START
     misc_feature    420..425
                     /label=STOP
     CDS             192..425
                     /label=BBa_I732006
     misc_feature    438..441
                     /label=conserved
     RBS             434..445
                     /label=BBa_B0034
     CDS             452..1171
                     /label=GFP protein
     CDS             452..1171
                     /label=BBa_E0040
     stem_loop       1191..1234
                     /label=stem_loop
     misc_feature    1180..1259
                     /label=BBa_B0010
     terminator      1180..1259
                     /label=BBa_B0010
     stem_loop       1275..1294
                     /label=T7 TE
     misc_feature    1301..1301
                     /label=stop
     polyA_site      1295..1308
                     /label=polya
     misc_feature    1268..1308
                     /label=BBa_B0012
     terminator      1268..1308
                     /label=BBa_B0012
ORIGIN
        1 tacccacaac ccaattcgag accggaactc gattgtatct gtagtgcttt agtagtggag
       61 tttacacttt atgcttccgg ctcgtataat gtgtggaaca tcccctttat ctatcgcctg
      121 tgcccactac atcaagccaa attaaacagg attaacagga tccggtacta gagaaagagg
      181 agaaatacta gatgaccatg attacggatt cactggccgt cgttttacaa cgtcgtgact
      241 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
      301 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
      361 gcgaatggcg ctttgcctgg tttccggcac cagaagcggt gccggaaagc tggctggagt
      421 aataatacta gagaaagagg agaaatacta gatgcgtaaa ggagaagaac ttttcactgg
      481 agttgtccca attcttgttg aattagatgg tgatgttaat gggcacaaat tttctgtcag
      541 tggagagggt gaaggtgatg caacatacgg aaaacttacc cttaaattta tttgcactac
      601 tggaaaacta cctgttccat ggccaacact tgtcactact ttcggttatg gtgttcaatg
      661 ctttgcgaga tacccagatc atatgaaaca gcatgacttt ttcaagagtg ccatgcccga
      721 aggttatgta caggaaagaa ctatattttt caaagatgac gggaactaca agacacgtgc
      781 tgaagtcaag tttgaaggtg atacccttgt taatagaatc gagttaaaag gtattgattt
      841 taaagaagat ggaaacattc ttggacacaa attggaatac aactataact cacacaatgt
      901 atacatcatg gcagacaaac aaaagaatgg aatcaaagtt aacttcaaaa ttagacacaa
      961 cattgaagat ggaagcgttc aactagcaga ccattatcaa caaaatactc caattggcga
     1021 tggccctgtc cttttaccag acaaccatta cctgtccaca caatctgccc tttcgaaaga
     1081 tcccaacgaa aagagagacc acatggtcct tcttgagttt gtaacagctg ctgggattac
     1141 acatggcatg gatgaactat acaaataata atactagagc caggcatcaa ataaaacgaa
     1201 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct
     1261 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttata
//