LOCUS       BBa_I732728             1304 bp    DNA     linear       29-May-2024
DEFINITION  P_U073O11+D062NUL + Double Reporters
ACCESSION   BBa_I732728
VERSION     BBa_I732728.1  
FEATURES             Location/Qualifiers
     promoter        57..93
                     /label=consensus elements
     misc_feature    1..20
                     /label=O1
     misc_feature    93..93
                     /label=+1
     promoter        1..161
                     /label=BBa_I732403
     misc_feature    174..177
                     /label=conserved
     RBS             170..181
                     /label=BBa_B0034
     CDS             188..421
                     /label=lacZ
     misc_feature    188..190
                     /label=START
     misc_feature    416..421
                     /label=STOP
     CDS             188..421
                     /label=BBa_I732006
     misc_feature    434..437
                     /label=conserved
     RBS             430..441
                     /label=BBa_B0034
     CDS             448..1167
                     /label=GFP protein
     CDS             448..1167
                     /label=BBa_E0040
     stem_loop       1187..1230
                     /label=stem_loop
     misc_feature    1176..1255
                     /label=BBa_B0010
     terminator      1176..1255
                     /label=BBa_B0010
     stem_loop       1271..1290
                     /label=T7 TE
     misc_feature    1297..1297
                     /label=stop
     polyA_site      1291..1304
                     /label=polya
     misc_feature    1264..1304
                     /label=BBa_B0012
     terminator      1264..1304
                     /label=BBa_B0012
ORIGIN
        1 aattgtgagc gctcacaatt gaactcgatt gtatctgtag tgctttagta gtggagttta
       61 cactttatgc ttccggctcg tataatgtgt ggaacatccc ctttatctat cgcctgtgcc
      121 cactacatca agccaaatta aacaggatta acaggatccg gtactagaga aagaggagaa
      181 atactagatg accatgatta cggattcact ggccgtcgtt ttacaacgtc gtgactggga
      241 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
      301 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
      361 atggcgcttt gcctggtttc cggcaccaga agcggtgccg gaaagctggc tggagtaata
      421 atactagaga aagaggagaa atactagatg cgtaaaggag aagaactttt cactggagtt
      481 gtcccaattc ttgttgaatt agatggtgat gttaatgggc acaaattttc tgtcagtgga
      541 gagggtgaag gtgatgcaac atacggaaaa cttaccctta aatttatttg cactactgga
      601 aaactacctg ttccatggcc aacacttgtc actactttcg gttatggtgt tcaatgcttt
      661 gcgagatacc cagatcatat gaaacagcat gactttttca agagtgccat gcccgaaggt
      721 tatgtacagg aaagaactat atttttcaaa gatgacggga actacaagac acgtgctgaa
      781 gtcaagtttg aaggtgatac ccttgttaat agaatcgagt taaaaggtat tgattttaaa
      841 gaagatggaa acattcttgg acacaaattg gaatacaact ataactcaca caatgtatac
      901 atcatggcag acaaacaaaa gaatggaatc aaagttaact tcaaaattag acacaacatt
      961 gaagatggaa gcgttcaact agcagaccat tatcaacaaa atactccaat tggcgatggc
     1021 cctgtccttt taccagacaa ccattacctg tccacacaat ctgccctttc gaaagatccc
     1081 aacgaaaaga gagaccacat ggtccttctt gagtttgtaa cagctgctgg gattacacat
     1141 ggcatggatg aactatacaa ataataatac tagagccagg catcaaataa aacgaaaggc
     1201 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta
     1261 gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tata
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