LOCUS       BBa_I732729             1292 bp    DNA     linear       28-May-2024
DEFINITION  P_U061O11+D062NUL + Double Reporters
ACCESSION   BBa_I732729
VERSION     BBa_I732729.1  
FEATURES             Location/Qualifiers
     misc_feature    32..35
                     /label=conserved
     RBS             28..39
                     /label=BBa_B0034
     CDS             46..279
                     /label=lacZ
     misc_feature    46..48
                     /label=START
     misc_feature    274..279
                     /label=STOP
     CDS             46..279
                     /label=BBa_I732006
     misc_feature    292..295
                     /label=conserved
     RBS             288..299
                     /label=BBa_B0034
     stem_loop       1084..1127
                     /label=stem_loop
     misc_feature    1073..1152
                     /label=BBa_B0010
     terminator      1073..1152
                     /label=BBa_B0010
     stem_loop       1168..1187
                     /label=T7 TE
     misc_feature    1194..1194
                     /label=stop
     polyA_site      1188..1201
                     /label=polya
     misc_feature    1161..1201
                     /label=BBa_B0012
     terminator      1161..1201
                     /label=BBa_B0012
     misc_feature    3..8
                     /label=BglII
     misc_feature    12..17
                     /label=BamHI
     misc_feature    28..39
                     /label=BBa_B0034
     misc_feature    32..35
                     /label=conserved
     misc_feature    46..279
                     /label=BBa_I732006
     CDS             46..279
                     /label=lacZ
     misc_feature    46..48
                     /label=START
     misc_feature    274..279
                     /label=STOP
     misc_feature    288..299
                     /label=BBa_B0034
     misc_feature    292..295
                     /label=conserved
     misc_feature    306..1064
                     /label=BBa_E0044
     misc_feature    1073..1152
                     /label=BBa_B0010
     stem_loop       1084..1127
                     /label=stem_loop
     misc_feature    1161..1201
                     /label=BBa_B0012
     stem_loop       1168..1187
                     /label=T7 TE
     polyA_site      1188..1201
                     /label=polya
     misc_feature    1194..1194
                     /label=stop
     promoter        1..149
                     /label=BBa_I732404
     misc_feature    162..165
                     /label=conserved
     RBS             158..169
                     /label=BBa_B0034
     CDS             176..409
                     /label=lacZ
     misc_feature    176..178
                     /label=START
     misc_feature    404..409
                     /label=STOP
     CDS             176..409
                     /label=BBa_I732006
     misc_feature    422..425
                     /label=conserved
     RBS             418..429
                     /label=BBa_B0034
     CDS             436..1155
                     /label=GFP protein
     CDS             436..1155
                     /label=BBa_E0040
     stem_loop       1175..1218
                     /label=stem_loop
     misc_feature    1164..1243
                     /label=BBa_B0010
     terminator      1164..1243
                     /label=BBa_B0010
     stem_loop       1259..1278
                     /label=T7 TE
     misc_feature    1285..1285
                     /label=stop
     polyA_site      1279..1292
                     /label=polya
     misc_feature    1252..1292
                     /label=BBa_B0012
     terminator      1252..1292
                     /label=BBa_B0012
ORIGIN
        1 aattgtgagc gctcacaatt atctgtagtg ctttagtagt ggagtttaca ctttatgctt
       61 ccggctcgta taatgtgtgg aacatcccct ttatctatcg cctgtgccca ctacatcaag
      121 ccaaattaaa caggattaac aggatccggt actagagaaa gaggagaaat actagatgac
      181 catgattacg gattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
      241 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
      301 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgctttgc
      361 ctggtttccg gcaccagaag cggtgccgga aagctggctg gagtaataat actagagaaa
      421 gaggagaaat actagatgcg taaaggagaa gaacttttca ctggagttgt cccaattctt
      481 gttgaattag atggtgatgt taatgggcac aaattttctg tcagtggaga gggtgaaggt
      541 gatgcaacat acggaaaact tacccttaaa tttatttgca ctactggaaa actacctgtt
      601 ccatggccaa cacttgtcac tactttcggt tatggtgttc aatgctttgc gagataccca
      661 gatcatatga aacagcatga ctttttcaag agtgccatgc ccgaaggtta tgtacaggaa
      721 agaactatat ttttcaaaga tgacgggaac tacaagacac gtgctgaagt caagtttgaa
      781 ggtgataccc ttgttaatag aatcgagtta aaaggtattg attttaaaga agatggaaac
      841 attcttggac acaaattgga atacaactat aactcacaca atgtatacat catggcagac
      901 aaacaaaaga atggaatcaa agttaacttc aaaattagac acaacattga agatggaagc
      961 gttcaactag cagaccatta tcaacaaaat actccaattg gcgatggccc tgtcctttta
     1021 ccagacaacc attacctgtc cacacaatct gccctttcga aagatcccaa cgaaaagaga
     1081 gaccacatgg tccttcttga gtttgtaaca gctgctggga ttacacatgg catggatgaa
     1141 ctatacaaat aataatacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga
     1201 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg
     1261 ctcaccttcg ggtgggcctt tctgcgttta ta
//