LOCUS       BBa_I732732             1268 bp    DNA     linear       28-May-2024
DEFINITION  P_U097NUL+D002O22 + Double Reporters
ACCESSION   BBa_I732732
VERSION     BBa_I732732.1  
FEATURES             Location/Qualifiers
     misc_feature    32..35
                     /label=conserved
     RBS             28..39
                     /label=BBa_B0034
     CDS             46..279
                     /label=lacZ
     misc_feature    46..48
                     /label=START
     misc_feature    274..279
                     /label=STOP
     CDS             46..279
                     /label=BBa_I732006
     misc_feature    292..295
                     /label=conserved
     RBS             288..299
                     /label=BBa_B0034
     stem_loop       1084..1127
                     /label=stem_loop
     misc_feature    1073..1152
                     /label=BBa_B0010
     terminator      1073..1152
                     /label=BBa_B0010
     stem_loop       1168..1187
                     /label=T7 TE
     misc_feature    1194..1194
                     /label=stop
     polyA_site      1188..1201
                     /label=polya
     misc_feature    1161..1201
                     /label=BBa_B0012
     terminator      1161..1201
                     /label=BBa_B0012
     misc_feature    3..8
                     /label=BglII
     misc_feature    12..17
                     /label=BamHI
     misc_feature    1..19
                     /label=BBa_I732007
     misc_feature    32..35
                     /label=conserved
     misc_feature    28..39
                     /label=BBa_B0034
     misc_feature    46..48
                     /label=START
     CDS             46..279
                     /label=lacZ
     misc_feature    274..279
                     /label=STOP
     misc_feature    46..279
                     /label=BBa_I732006
     misc_feature    292..295
                     /label=conserved
     misc_feature    288..299
                     /label=BBa_B0034
     misc_feature    306..1064
                     /label=BBa_E0044
     misc_feature    1073..1152
                     /label=BBa_B0010
     stem_loop       1084..1127
                     /label=stem_loop
     misc_feature    1161..1201
                     /label=BBa_B0012
     stem_loop       1168..1187
                     /label=T7 TE
     polyA_site      1188..1201
                     /label=polya
     misc_feature    1194..1194
                     /label=stop
     promoter        1..125
                     /label=BBa_I732407
     misc_feature    138..141
                     /label=conserved
     RBS             134..145
                     /label=BBa_B0034
     CDS             152..385
                     /label=lacZ
     misc_feature    152..154
                     /label=START
     misc_feature    380..385
                     /label=STOP
     CDS             152..385
                     /label=BBa_I732006
     misc_feature    398..401
                     /label=conserved
     RBS             394..405
                     /label=BBa_B0034
     CDS             412..1131
                     /label=GFP protein
     CDS             412..1131
                     /label=BBa_E0040
     stem_loop       1151..1194
                     /label=stem_loop
     misc_feature    1140..1219
                     /label=BBa_B0010
     terminator      1140..1219
                     /label=BBa_B0010
     stem_loop       1235..1254
                     /label=T7 TE
     misc_feature    1261..1261
                     /label=stop
     polyA_site      1255..1268
                     /label=polya
     misc_feature    1228..1268
                     /label=BBa_B0012
     terminator      1228..1268
                     /label=BBa_B0012
ORIGIN
        1 tacccacaac ccaattcgag accggaactc gattgtatct gtagtgcttt agtagtggag
       61 tttacacttt atgcttccgg ctcgtataat gtgtggaaat tgtaagcgct tacaattgga
      121 tccggtacta gagaaagagg agaaatacta gatgaccatg attacggatt cactggccgt
      181 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
      241 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
      301 acagttgcgc agcctgaatg gcgaatggcg ctttgcctgg tttccggcac cagaagcggt
      361 gccggaaagc tggctggagt aataatacta gagaaagagg agaaatacta gatgcgtaaa
      421 ggagaagaac ttttcactgg agttgtccca attcttgttg aattagatgg tgatgttaat
      481 gggcacaaat tttctgtcag tggagagggt gaaggtgatg caacatacgg aaaacttacc
      541 cttaaattta tttgcactac tggaaaacta cctgttccat ggccaacact tgtcactact
      601 ttcggttatg gtgttcaatg ctttgcgaga tacccagatc atatgaaaca gcatgacttt
      661 ttcaagagtg ccatgcccga aggttatgta caggaaagaa ctatattttt caaagatgac
      721 gggaactaca agacacgtgc tgaagtcaag tttgaaggtg atacccttgt taatagaatc
      781 gagttaaaag gtattgattt taaagaagat ggaaacattc ttggacacaa attggaatac
      841 aactataact cacacaatgt atacatcatg gcagacaaac aaaagaatgg aatcaaagtt
      901 aacttcaaaa ttagacacaa cattgaagat ggaagcgttc aactagcaga ccattatcaa
      961 caaaatactc caattggcga tggccctgtc cttttaccag acaaccatta cctgtccaca
     1021 caatctgccc tttcgaaaga tcccaacgaa aagagagacc acatggtcct tcttgagttt
     1081 gtaacagctg ctgggattac acatggcatg gatgaactat acaaataata atactagagc
     1141 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg
     1201 tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg
     1261 cgtttata
//