LOCUS       BBa_I732733             1280 bp    DNA     linear       28-May-2024
DEFINITION  P_U097NUL+D014O22 + Double Reporters
ACCESSION   BBa_I732733
VERSION     BBa_I732733.1  
FEATURES             Location/Qualifiers
     misc_feature    32..35
                     /label=conserved
     RBS             28..39
                     /label=BBa_B0034
     CDS             46..279
                     /label=lacZ
     misc_feature    46..48
                     /label=START
     misc_feature    274..279
                     /label=STOP
     CDS             46..279
                     /label=BBa_I732006
     misc_feature    292..295
                     /label=conserved
     RBS             288..299
                     /label=BBa_B0034
     stem_loop       1031..1074
                     /label=stem_loop
     misc_feature    1020..1099
                     /label=BBa_B0010
     terminator      1020..1099
                     /label=BBa_B0010
     stem_loop       1115..1134
                     /label=T7 TE
     misc_feature    1141..1141
                     /label=stop
     polyA_site      1135..1148
                     /label=polya
     misc_feature    1108..1148
                     /label=BBa_B0012
     terminator      1108..1148
                     /label=BBa_B0012
     misc_feature    3..8
                     /label=BglII
     misc_feature    12..17
                     /label=BamHI
     misc_feature    1..19
                     /label=BBa_I732007
     misc_feature    32..35
                     /label=conserved
     misc_feature    28..39
                     /label=BBa_B0034
     misc_feature    46..48
                     /label=START
     CDS             46..279
                     /label=lacZ
     misc_feature    274..279
                     /label=STOP
     misc_feature    46..279
                     /label=BBa_I732006
     misc_feature    292..295
                     /label=conserved
     misc_feature    288..299
                     /label=BBa_B0034
     CDS             306..980
                     /label=mrfp1
     misc_feature    306..986
                     /label=BBa_E1010
     misc_feature    987..1011
                     /label=barcode
     misc_feature    1020..1099
                     /label=BBa_B0010
     stem_loop       1031..1074
                     /label=stem_loop
     misc_feature    1108..1148
                     /label=BBa_B0012
     stem_loop       1115..1134
                     /label=T7 TE
     polyA_site      1135..1148
                     /label=polya
     misc_feature    1141..1141
                     /label=stop
     promoter        1..137
                     /label=BBa_I732408
     misc_feature    150..153
                     /label=conserved
     RBS             146..157
                     /label=BBa_B0034
     CDS             164..397
                     /label=lacZ
     misc_feature    164..166
                     /label=START
     misc_feature    392..397
                     /label=STOP
     CDS             164..397
                     /label=BBa_I732006
     misc_feature    410..413
                     /label=conserved
     RBS             406..417
                     /label=BBa_B0034
     CDS             424..1143
                     /label=GFP protein
     CDS             424..1143
                     /label=BBa_E0040
     stem_loop       1163..1206
                     /label=stem_loop
     misc_feature    1152..1231
                     /label=BBa_B0010
     terminator      1152..1231
                     /label=BBa_B0010
     stem_loop       1247..1266
                     /label=T7 TE
     misc_feature    1273..1273
                     /label=stop
     polyA_site      1267..1280
                     /label=polya
     misc_feature    1240..1280
                     /label=BBa_B0012
     terminator      1240..1280
                     /label=BBa_B0012
     misc_feature    146..1280
                     /label=BBa_I732093
ORIGIN
        1 tacccacaac ccaattcgag accggaactc gattgtatct gtagtgcttt agtagtggag
       61 tttacacttt atgcttccgg ctcgtataat gtgtggaaca tcccctttaa attgtaagcg
      121 cttacaattg gatccggtac tagagaaaga ggagaaatac tagatgacca tgattacgga
      181 ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa
      241 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga
      301 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg cgctttgcct ggtttccggc
      361 accagaagcg gtgccggaaa gctggctgga gtaataatac tagagaaaga ggagaaatac
      421 tagatgcgta aaggagaaga acttttcact ggagttgtcc caattcttgt tgaattagat
      481 ggtgatgtta atgggcacaa attttctgtc agtggagagg gtgaaggtga tgcaacatac
      541 ggaaaactta cccttaaatt tatttgcact actggaaaac tacctgttcc atggccaaca
      601 cttgtcacta ctttcggtta tggtgttcaa tgctttgcga gatacccaga tcatatgaaa
      661 cagcatgact ttttcaagag tgccatgccc gaaggttatg tacaggaaag aactatattt
      721 ttcaaagatg acgggaacta caagacacgt gctgaagtca agtttgaagg tgataccctt
      781 gttaatagaa tcgagttaaa aggtattgat tttaaagaag atggaaacat tcttggacac
      841 aaattggaat acaactataa ctcacacaat gtatacatca tggcagacaa acaaaagaat
      901 ggaatcaaag ttaacttcaa aattagacac aacattgaag atggaagcgt tcaactagca
      961 gaccattatc aacaaaatac tccaattggc gatggccctg tccttttacc agacaaccat
     1021 tacctgtcca cacaatctgc cctttcgaaa gatcccaacg aaaagagaga ccacatggtc
     1081 cttcttgagt ttgtaacagc tgctgggatt acacatggca tggatgaact atacaaataa
     1141 taatactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
     1201 ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg
     1261 tgggcctttc tgcgtttata
//